Insertion of tail-anchored proteins into the endoplasmic reticulum membrane (Homo sapiens)

From WikiPathways

You do not have permission to edit pages, for the following reasons:

• You do not have permission to edit pages in the Pathway namespace.
• You are not allowed to execute the action you have requested.

You can view and copy the source of this page:

<?xml version="1.0" encoding="UTF-8"?> <Pathway xmlns="http://pathvisio.org/GPML/2013a" Name="Insertion of tail-anchored proteins into the endoplasmic reticulum membrane" Organism="Homo sapiens"> <Comment Source="WikiPathways-description">Tail-anchored (TA) proteins have a hydrophobic transmembrane domain (TMD) located near the C-terminus ("tail") of the protein. Depending on the nature of the TMD, TA proteins can be inserted into the endoplasmic reticulum (ER) membrane by at least 4 mechanisms: cotranslational insertion by the signal recognition particle (SRP), post-translational insertion by ASNA1 (TRC40), post-translational insertion by the SRP, and post-translational insertion by a SRP-independent mechanism (SND) (Casson et al. 2017, reviewed in Borgese and Fasana 2011, Casson et al. 2016, Aviram et al. 2016, Chio et al. 2017). Much of the information about the mammalian system of insertion by ASNA1 (TRC40) has been inferred from the Saccharomyces cerevisiae homologue Get3.&lt;br&gt; Prior to post-translational insertion by ASNA1, SGTA binds the transmembrane domain of the substrate TA protein immediately after translation (Leznicki et al. 2011, Leznicki and High 2012, Xu et al. 2012, Wunderly et al. 2014, Shao et al. 2017), the SGTA:TA protein complex then binds the BAG6 complex (BAG6:GET4:UBL4A) via UBL4A (Winnefeld et al. 2006, Chartron et al. 2012, Xu et al. 2012, Leznicki et al. 2013, Mock et al. 2015, Kuwabara et al. 2015, Shao et al. 2017), and the TA protein is transferred to ASNA1 (Mariappan et al. 2010, Leznicki et al. 2011, Shao et al. 2017), also bound by the BAG6 complex via UBL4A. The ASNA1:TA protein complex then docks at the WRB:CAMLG (WRB:CAML) complex located in the ER membrane and the TA protein is inserted into the ER membrane by an uncharacterized mechanism that involves ATP and the transmembrane domain insertase activity of the WRB:CAML complex (Vilardi et al. 2011, Vilardi et al. 2014, Vogl et al. 2016, and inferred from yeast in Wang et al. 2014).&lt;br&gt;Misfolded TA proteins, overexpressed TA proteins, and membrane proteins mislocalized in the cytosol bind SGTA but are not efficiently transferred to ASNA1 and, instead, are retained by BAG6 which recruits RNF126 to ubiquitinate them, targeting them for degradation by the proteasome (Wang et al. 2011, Leznicki and High 2012, Xu et al. 2012, Rodrigo-Brenni et al. 2014, Wunderly et al. 2014, Shao et al. 2017, reviewed in Lee and Ye 2013, Casson et al. 2016, Krysztofinska et al. 2016, Guna and Hegde 2018). View original pathway at [http://www.reactome.org/PathwayBrowser/#DIAGRAM=9609523 Reactome].</Comment> <Comment Source="Reactome-Converter">Pathway is converted from Reactome ID: 9609523</Comment> <Comment Source="Reactome-version">Reactome version: 75</Comment> <Comment Source="Reactome Author">Reactome Author: May, Bruce</Comment> <BiopaxRef>d48</BiopaxRef> <BiopaxRef>a18</BiopaxRef> <BiopaxRef>aa1</BiopaxRef> <BiopaxRef>af2</BiopaxRef> <BiopaxRef>a00</BiopaxRef> <BiopaxRef>c71</BiopaxRef> <BiopaxRef>c8c</BiopaxRef> <BiopaxRef>a36</BiopaxRef> <BiopaxRef>c19</BiopaxRef> <BiopaxRef>cc3</BiopaxRef> <BiopaxRef>d58</BiopaxRef> <BiopaxRef>deb</BiopaxRef> <BiopaxRef>e5e</BiopaxRef> <BiopaxRef>e13</BiopaxRef> <BiopaxRef>fe5</BiopaxRef> <BiopaxRef>a98</BiopaxRef> <BiopaxRef>cc8</BiopaxRef> <BiopaxRef>ad5</BiopaxRef> <BiopaxRef>e91</BiopaxRef> <BiopaxRef>d11</BiopaxRef> <BiopaxRef>dd2</BiopaxRef> <BiopaxRef>fe9</BiopaxRef> <BiopaxRef>f82</BiopaxRef> <BiopaxRef>c9e</BiopaxRef> <BiopaxRef>f47</BiopaxRef> <Attribute Key="reactome_id" Value="9609523" /> <Graphics BoardWidth="880.0" BoardHeight="1398.0" /> <DataNode TextLabel="ATP" GraphId="a0586" Type="Metabolite"> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="392.0" CenterY="461.0" Width="40.0" Height="30.0" ZOrder="32768" FontSize="10" Valign="Middle" Color="0000ff" /> <Xref Database="ChEBI" ID="CHEBI:30616" /> </DataNode> <DataNode TextLabel="UBE2J2-1 " GraphId="a0db1" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="978.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q8N2K1-1" /> </DataNode> <DataNode TextLabel="HMOX1 " GraphId="a2448" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1138.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P09601" /> </DataNode> <DataNode TextLabel="SEC61B " GraphId="a3667" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="1018.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60468" /> </DataNode> <DataNode TextLabel="PRNP " GraphId="a38f3" Type="Protein" GroupRef="c82bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617598" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="520.0" CenterY="1098.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P04156" /> </DataNode> <DataNode TextLabel="STX5 " GraphId="a3b5d" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="958.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q13190" /> </DataNode> <DataNode TextLabel="EMD " GraphId="a45f1" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="778.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P50402" /> </DataNode> <DataNode TextLabel="WRB " GraphId="a4783" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1038.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O00258" /> </DataNode> <DataNode TextLabel="ASNA1 " GraphId="a4d03" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="240.0" CenterY="1038.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43681" /> </DataNode> <DataNode TextLabel="VAMP2 " GraphId="a59bb" Type="Protein" GroupRef="d8390"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617594" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="738.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P63027" /> </DataNode> <DataNode TextLabel="UBL4A " GraphId="a5fd6" Type="Protein" GroupRef="c82bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617598" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="1018.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P11441" /> </DataNode> <DataNode TextLabel="VAPA " GraphId="a6154" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1318.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9P0L0" /> </DataNode> <DataNode TextLabel="Tail-anchored&#xA;protein:SGTA:BAG6:GET4:UBL4A:ASNA1:ATP" GraphId="a640b" Type="Complex"> <BiopaxRef>dd2</BiopaxRef> <BiopaxRef>c8c</BiopaxRef> <BiopaxRef>aa1</BiopaxRef> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="218.0" CenterY="316.0" Width="214.0" Height="38.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9610487" /> </DataNode> <DataNode TextLabel="HMOX1 " GraphId="a641e" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="978.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P09601" /> </DataNode> <DataNode TextLabel="SGTA " GraphId="a8870" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="758.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43765" /> </DataNode> <DataNode TextLabel="UBE2J2-1 " GraphId="ac2db" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="1118.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q8N2K1-1" /> </DataNode> <DataNode TextLabel="ALDH3A2-1 " GraphId="ace90" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="778.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P51648-1" /> </DataNode> <DataNode TextLabel="ALDH3A2-1 " GraphId="ae50a" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="698.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P51648-1" /> </DataNode> <DataNode TextLabel="VAMP2 " GraphId="aebfd" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="998.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P63027" /> </DataNode> <DataNode TextLabel="OTOF " GraphId="aef43" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="858.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9HC10" /> </DataNode> <DataNode TextLabel="SEC61B " GraphId="af7c7" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="838.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60468" /> </DataNode> <DataNode TextLabel="VAMP2 " GraphId="b011b" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="918.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P63027" /> </DataNode> <DataNode TextLabel="VAMP2 " GraphId="b127f" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="958.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P63027" /> </DataNode> <DataNode TextLabel="CYB5A-1 " GraphId="b20a8" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="718.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P00167-1" /> </DataNode> <DataNode TextLabel="ASNA1 " GraphId="b23eb" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="380.0" CenterY="698.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43681" /> </DataNode> <DataNode TextLabel="EMD " GraphId="b2d11" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="818.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P50402" /> </DataNode> <DataNode TextLabel="ATP " GraphId="b2e48" Type="Metabolite" GroupRef="c82bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617598" /> <Attribute Key="copies_num" Value="4" /> <Graphics CenterX="520.0" CenterY="1158.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" Color="0000ff" /> <Xref Database="ChEBI" ID="CHEBI:30616" /> </DataNode> <DataNode TextLabel="SEC61G " GraphId="b3082" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="898.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60059" /> </DataNode> <DataNode TextLabel="OTOF " GraphId="b4355" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="778.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9HC10" /> </DataNode> <DataNode TextLabel="CYB5A-1 " GraphId="b6078" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="938.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P00167-1" /> </DataNode> <DataNode TextLabel="HMOX1 " GraphId="b6ed5" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="838.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P09601" /> </DataNode> <DataNode TextLabel="SGTA " GraphId="b8299" Type="Protein" GroupRef="c82bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617598" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="520.0" CenterY="1058.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43765" /> </DataNode> <DataNode TextLabel="SEC61B " GraphId="b83c9" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1178.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60468" /> </DataNode> <DataNode TextLabel="OTOF " GraphId="b864b" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="998.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9HC10" /> </DataNode> <DataNode TextLabel="SERP1 " GraphId="b8dfe" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="918.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9Y6X1" /> </DataNode> <DataNode TextLabel="ALDH3A2-1 " GraphId="b94b5" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="738.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P51648-1" /> </DataNode> <DataNode TextLabel="SEC61G " GraphId="ba298" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="858.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60059" /> </DataNode> <DataNode TextLabel="VAMP2 " GraphId="ba33f" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1298.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P63027" /> </DataNode> <DataNode TextLabel="STX1A " GraphId="bb7cf" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="1078.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q16623" /> </DataNode> <DataNode TextLabel="STX5 " GraphId="bc321" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1258.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q13190" /> </DataNode> <DataNode TextLabel="ALDH3A2-1 " GraphId="bf4ca" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="918.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P51648-1" /> </DataNode> <DataNode TextLabel="PRNP " GraphId="bf643" Type="Protein" GroupRef="c5451"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617602" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="660.0" CenterY="838.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P04156" /> </DataNode> <DataNode TextLabel="VAPA " GraphId="c01fa" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="938.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9P0L0" /> </DataNode> <DataNode TextLabel="STX5 " GraphId="c08fd" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="1098.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q13190" /> </DataNode> <DataNode TextLabel="VAMP2 " GraphId="c0f23" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="998.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P63027" /> </DataNode> <DataNode TextLabel="SEC61B " GraphId="c1660" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="798.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60468" /> </DataNode> <DataNode TextLabel="BAG6 " GraphId="c2510" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="240.0" CenterY="698.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P46379" /> </DataNode> <DataNode TextLabel="BAG6 " GraphId="c275b" Type="Protein" GroupRef="c82bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617598" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="998.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P46379" /> </DataNode> <DataNode TextLabel="SERP1 " GraphId="c32b0" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="878.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9Y6X1" /> </DataNode> <DataNode TextLabel="ADP " GraphId="c58fa" Type="Metabolite" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="800.0" CenterY="978.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" Color="0000ff" /> <Xref Database="ChEBI" ID="CHEBI:456216" /> </DataNode> <DataNode TextLabel="SERP1 " GraphId="c5afe" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1218.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9Y6X1" /> </DataNode> <DataNode TextLabel="EMD " GraphId="c5ca4" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="818.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P50402" /> </DataNode> <DataNode TextLabel="STX1A " GraphId="c5d0f" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="938.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q16623" /> </DataNode> <DataNode TextLabel="SEC61G " GraphId="c6162" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1198.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60059" /> </DataNode> <DataNode TextLabel="SGTA " GraphId="c63e1" Type="Protein" GroupRef="ac236"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610464" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="698.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43765" /> </DataNode> <DataNode TextLabel="EMD " GraphId="c924b" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="958.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P50402" /> </DataNode> <DataNode TextLabel="OTOF " GraphId="c9afd" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1158.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9HC10" /> </DataNode> <DataNode TextLabel="VAMP2 " GraphId="ca1de" Type="Protein" GroupRef="c5451"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617602" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="660.0" CenterY="858.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P63027" /> </DataNode> <DataNode TextLabel="ALDH3A2-1 " GraphId="cae04" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1078.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P51648-1" /> </DataNode> <DataNode TextLabel="SERP1 " GraphId="caf55" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="838.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9Y6X1" /> </DataNode> <DataNode TextLabel="ASNA1 " GraphId="cb3e6" Type="Protein" GroupRef="f29ea"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609895" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="1078.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43681" /> </DataNode> <DataNode TextLabel="Tail-anchored&#xA;protein:SGTA dimer" GraphId="cb421" Type="Complex"> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="215.0" CenterY="204.0" Width="232.0" Height="26.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9610465" /> </DataNode> <DataNode TextLabel="VAPA " GraphId="cb7ca" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="1158.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9P0L0" /> </DataNode> <DataNode TextLabel="CAMLG " GraphId="cc1ea" Type="Protein" GroupRef="be72f"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609864" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="240.0" CenterY="1138.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P49069" /> </DataNode> <DataNode TextLabel="CYB5A-1 " GraphId="ce4ca" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="798.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P00167-1" /> </DataNode> <DataNode TextLabel="EMD " GraphId="cea57" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="738.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P50402" /> </DataNode> <DataNode TextLabel="ALDH3A2-1 " GraphId="d007c" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="778.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P51648-1" /> </DataNode> <DataNode TextLabel="UBE2J2-1 " GraphId="d0f8e" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="978.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q8N2K1-1" /> </DataNode> <DataNode TextLabel="CYB5A-1 " GraphId="d2046" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="758.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P00167-1" /> </DataNode> <DataNode TextLabel="WRB:CAMLG" GraphId="d325c" Type="Complex"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Graphics CenterX="172.5" CenterY="525.0" Width="93.0" Height="26.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9609864" /> </DataNode> <DataNode TextLabel="CAMLG " GraphId="d37b5" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1058.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P49069" /> </DataNode> <DataNode TextLabel="SGTA " GraphId="d46e2" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="758.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43765" /> </DataNode> <DataNode TextLabel="STX5 " GraphId="d4813" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="878.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q13190" /> </DataNode> <DataNode TextLabel="ALDH3A2-1 " GraphId="d57cb" Type="Protein" GroupRef="fb484"> <BiopaxRef>df8</BiopaxRef> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="698.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P51648-1" /> </DataNode> <DataNode TextLabel="SEC61G " GraphId="d5a85" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="818.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60059" /> </DataNode> <DataNode TextLabel="UBE2J2-1 " GraphId="d6415" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="898.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q8N2K1-1" /> </DataNode> <DataNode TextLabel="HMOX1 " GraphId="d661a" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="838.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P09601" /> </DataNode> <DataNode TextLabel="Tail-anchored&#xA;protein:ASNA1:ATP" GraphId="d66b2" Type="Complex"> <BiopaxRef>ce2</BiopaxRef> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="111.0" CenterY="360.0" Width="156.0" Height="38.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9609923" /> </DataNode> <DataNode TextLabel="Mislocalized&#xA;membrane&#xA;protein:SGTA dimer" GraphId="d6cd0" Type="Complex"> <BiopaxRef>aa1</BiopaxRef> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="609.5" CenterY="171.5" Width="295.0" Height="29.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9617602" /> </DataNode> <DataNode TextLabel="UBE2J2-1 " GraphId="d7d64" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="898.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q8N2K1-1" /> </DataNode> <DataNode TextLabel="GET4 " GraphId="d852d" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="240.0" CenterY="738.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q7L5D6" /> </DataNode> <DataNode TextLabel="UBE2J2-1 " GraphId="da020" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="938.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q8N2K1-1" /> </DataNode> <DataNode TextLabel="ATP " GraphId="da344" Type="Metabolite" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="718.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" Color="0000ff" /> <Xref Database="ChEBI" ID="CHEBI:30616" /> </DataNode> <DataNode TextLabel="SERP1 " GraphId="da6a4" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="918.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9Y6X1" /> </DataNode> <DataNode TextLabel="VAPA " GraphId="da731" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="1018.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9P0L0" /> </DataNode> <DataNode TextLabel="VAMP2 " GraphId="daa1a" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="1138.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P63027" /> </DataNode> <DataNode TextLabel="EMD " GraphId="db52a" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1118.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P50402" /> </DataNode> <DataNode TextLabel="VAPA " GraphId="dd6e7" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="938.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9P0L0" /> </DataNode> <DataNode TextLabel="CYB5A-1 " GraphId="df065" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1098.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P00167-1" /> </DataNode> <DataNode TextLabel="BAG6 " GraphId="e2432" Type="Protein" GroupRef="dae8b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609902" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="100.0" CenterY="1158.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P46379" /> </DataNode> <DataNode TextLabel="VAMP2 " GraphId="e24a0" Type="Protein" GroupRef="c82bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617598" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="520.0" CenterY="1118.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P63027" /> </DataNode> <DataNode TextLabel="VAPA " GraphId="e2744" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="1018.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9P0L0" /> </DataNode> <DataNode TextLabel="SEC61G " GraphId="e296e" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="898.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60059" /> </DataNode> <DataNode TextLabel="HMOX1 " GraphId="e2f1c" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="798.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P09601" /> </DataNode> <DataNode TextLabel="STX1A " GraphId="e3908" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="858.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q16623" /> </DataNode> <DataNode TextLabel="SEC61B " GraphId="e3e7d" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="878.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60468" /> </DataNode> <DataNode TextLabel="Mislocalized&#xA;membrane protein" GraphId="e4faa" Type="Complex"> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="643.0" CenterY="57.5" Width="210.0" Height="23.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="RoundedRectangle" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9617594" /> </DataNode> <DataNode TextLabel="ASNA1 " GraphId="e52ff" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="800.0" CenterY="958.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43681" /> </DataNode> <DataNode TextLabel="STX1A " GraphId="e56ac" Type="Protein" GroupRef="dc45b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610465" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="100.0" CenterY="938.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q16623" /> </DataNode> <DataNode TextLabel="Mislocalized&#xA;membrane&#xA;protein:SGTA:BAG6:GET4:UBL4A:ASNA1:ATP" GraphId="e62e9" Type="Complex"> <BiopaxRef>aa1</BiopaxRef> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="626.5" CenterY="317.5" Width="245.0" Height="43.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9617598" /> </DataNode> <DataNode TextLabel="STX5 " GraphId="e6af9" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="918.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q13190" /> </DataNode> <DataNode TextLabel="ADP" GraphId="e7958" Type="Metabolite"> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="528.0" CenterY="462.0" Width="42.0" Height="30.0" ZOrder="32768" FontSize="10" Valign="Middle" Color="0000ff" /> <Xref Database="ChEBI" ID="CHEBI:456216" /> </DataNode> <DataNode TextLabel="ATP " GraphId="e7c5d" Type="Metabolite" GroupRef="f29ea"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609895" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="100.0" CenterY="1098.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" Color="0000ff" /> <Xref Database="ChEBI" ID="CHEBI:30616" /> </DataNode> <DataNode TextLabel="ADP " GraphId="e9374" Type="Metabolite" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="1358.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" Color="0000ff" /> <Xref Database="ChEBI" ID="CHEBI:456216" /> </DataNode> <DataNode TextLabel="Pi" GraphId="e98a4" Type="Metabolite"> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="293.0" CenterY="395.0" Width="26.0" Height="30.0" ZOrder="32768" FontSize="10" Valign="Middle" Color="0000ff" /> <Xref Database="ChEBI" ID="CHEBI:43474" /> </DataNode> <DataNode TextLabel="BAG6:GET4:UBL4A" GraphId="e9e01" Type="Complex"> <BiopaxRef>efb</BiopaxRef> <BiopaxRef>dd2</BiopaxRef> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="373.0" CenterY="258.0" Width="132.0" Height="24.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9609902" /> </DataNode> <DataNode TextLabel="ASNA1 " GraphId="ebada" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="380.0" CenterY="1338.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43681" /> </DataNode> <DataNode TextLabel="Tail-anchored&#xA;protein:ASNA1:ADP:WRB:CAMLG" GraphId="ebdbd" Type="Complex"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Graphics CenterX="324.5" CenterY="515.0" Width="173.0" Height="38.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9609912" /> </DataNode> <DataNode TextLabel="Tail-anchored&#xA;proteins" GraphId="ec210" Type="Complex"> <BiopaxRef>fe5</BiopaxRef> <BiopaxRef>c1b</BiopaxRef> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="158.5" CenterY="61.5" Width="167.0" Height="29.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="RoundedRectangle" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9609909" /> </DataNode> <DataNode TextLabel="OTOF " GraphId="ec575" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="778.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9HC10" /> </DataNode> <DataNode TextLabel="Tail-anchored&#xA;protein:ASNA1:ADP" GraphId="ecec4" Type="Complex"> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="210.5" CenterY="437.0" Width="141.0" Height="38.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9628396" /> </DataNode> <DataNode TextLabel="HMOX1 " GraphId="ed5c4" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="758.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P09601" /> </DataNode> <DataNode TextLabel="ATP " GraphId="ed874" Type="Metabolite" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="4" /> <Graphics CenterX="240.0" CenterY="1058.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" Color="0000ff" /> <Xref Database="ChEBI" ID="CHEBI:30616" /> </DataNode> <DataNode TextLabel="VAMP2 " GraphId="edfb6" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="918.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P63027" /> </DataNode> <DataNode TextLabel="UBL4A " GraphId="ee328" Type="Protein" GroupRef="dae8b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609902" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="100.0" CenterY="1178.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P11441" /> </DataNode> <DataNode TextLabel="SEC61B " GraphId="f02ff" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="798.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60468" /> </DataNode> <DataNode TextLabel="Tail-anchored&#xA;proteins" GraphId="f057a" Type="Complex"> <BiopaxRef>fe5</BiopaxRef> <BiopaxRef>c1b</BiopaxRef> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Graphics CenterX="597.0" CenterY="527.5" Width="162.0" Height="35.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="RoundedRectangle" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9609893" /> </DataNode> <DataNode TextLabel="OTOF " GraphId="f1f9f" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="858.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9HC10" /> </DataNode> <DataNode TextLabel="ASNA1 " GraphId="f2e32" Type="Protein" GroupRef="c82bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617598" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="520.0" CenterY="1138.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43681" /> </DataNode> <DataNode TextLabel="CYB5A-1 " GraphId="f2ec3" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="798.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P00167-1" /> </DataNode> <DataNode TextLabel="SEC61G " GraphId="f36cf" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="1038.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60059" /> </DataNode> <DataNode TextLabel="GET4 " GraphId="f3fdd" Type="Protein" GroupRef="dae8b"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609902" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="100.0" CenterY="1198.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q7L5D6" /> </DataNode> <DataNode TextLabel="ASNA1:ATP dimer" GraphId="f418f" Type="Complex"> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="497.5" CenterY="221.0" Width="133.0" Height="24.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9609895" /> </DataNode> <DataNode TextLabel="OTOF " GraphId="f50ce" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="818.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9HC10" /> </DataNode> <DataNode TextLabel="SERP1 " GraphId="f5254" Type="Protein" GroupRef="b74ae"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609909" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="1058.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9Y6X1" /> </DataNode> <DataNode TextLabel="APP-C99 " GraphId="f5c49" Type="Protein" GroupRef="d8390"> <BiopaxRef>c71</BiopaxRef> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617594" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="698.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P05067" /> </DataNode> <DataNode TextLabel="UBL4A " GraphId="f6144" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="240.0" CenterY="718.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P11441" /> </DataNode> <DataNode TextLabel="STX1A " GraphId="f63ed" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1238.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q16623" /> </DataNode> <DataNode TextLabel="EMD " GraphId="f68ef" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="738.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P50402" /> </DataNode> <DataNode TextLabel="HMOX1 " GraphId="f713e" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="758.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P09601" /> </DataNode> <DataNode TextLabel="SGTA " GraphId="f73ff" Type="Protein" GroupRef="c5451"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617602" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="660.0" CenterY="798.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O43765" /> </DataNode> <DataNode TextLabel="GET4 " GraphId="f877c" Type="Protein" GroupRef="c82bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617598" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="1038.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q7L5D6" /> </DataNode> <DataNode TextLabel="SEC61B " GraphId="f892e" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="878.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60468" /> </DataNode> <DataNode TextLabel="APP-C99 " GraphId="f8ba6" Type="Protein" GroupRef="c82bc"> <BiopaxRef>c71</BiopaxRef> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617598" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="520.0" CenterY="1078.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P05067" /> </DataNode> <DataNode TextLabel="SGTA dimer" GraphId="f8ea0" Type="Complex"> <BiopaxRef>e78</BiopaxRef> <BiopaxRef>c1e</BiopaxRef> <Attribute Key="cellular_location" Value="cytosol" /> <Graphics CenterX="388.5" CenterY="33.5" Width="103.0" Height="25.0" ZOrder="32768" FontSize="10" Valign="Middle" ShapeType="Hexagon" Color="a52a2a" /> <Xref Database="Reactome" ID="R-HSA-9610464" /> </DataNode> <DataNode TextLabel="STX5 " GraphId="fad97" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="878.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q13190" /> </DataNode> <DataNode TextLabel="SERP1 " GraphId="fb33f" Type="Protein" GroupRef="f44ad"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9628396" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="800.0" CenterY="838.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9Y6X1" /> </DataNode> <DataNode TextLabel="WRB " GraphId="fbc49" Type="Protein" GroupRef="be72f"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609864" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="240.0" CenterY="1118.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="O00258" /> </DataNode> <DataNode TextLabel="CYB5A-1 " GraphId="fcd4b" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="718.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P00167-1" /> </DataNode> <DataNode TextLabel="STX5 " GraphId="fce38" Type="Protein" GroupRef="dd7bc"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9610487" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="240.0" CenterY="958.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q13190" /> </DataNode> <DataNode TextLabel="STX1A " GraphId="fd041" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="898.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q16623" /> </DataNode> <DataNode TextLabel="UBE2J2-1 " GraphId="fd83a" Type="Protein" GroupRef="a889e"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609912" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="380.0" CenterY="1278.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q8N2K1-1" /> </DataNode> <DataNode TextLabel="STX1A " GraphId="fd888" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="858.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q16623" /> </DataNode> <DataNode TextLabel="SEC61G " GraphId="fe19b" Type="Protein" GroupRef="fb484"> <Attribute Key="cellular_location" Value="endoplasmic reticulum membrane" /> <Attribute Key="complex_id" Value="R-HSA-9609893" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="520.0" CenterY="818.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P60059" /> </DataNode> <DataNode TextLabel="VAPA " GraphId="fe7dd" Type="Protein" GroupRef="cb890"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9609923" /> <Attribute Key="copies_num" Value="3" /> <Graphics CenterX="380.0" CenterY="978.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="Q9P0L0" /> </DataNode> <DataNode TextLabel="PRNP " GraphId="fea29" Type="Protein" GroupRef="d8390"> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617594" /> <Attribute Key="copies_num" Value="1" /> <Graphics CenterX="660.0" CenterY="718.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P04156" /> </DataNode> <DataNode TextLabel="APP-C99 " GraphId="ffc2c" Type="Protein" GroupRef="c5451"> <BiopaxRef>c71</BiopaxRef> <Attribute Key="cellular_location" Value="cytosol" /> <Attribute Key="complex_id" Value="R-HSA-9617602" /> <Attribute Key="copies_num" Value="2" /> <Graphics CenterX="660.0" CenterY="818.0" Width="100.0" Height="20.0" ZOrder="32768" FontSize="10" Valign="Middle" /> <Xref Database="Uniprot-TrEMBL" ID="P05067" /> </DataNode> <Interaction GraphId="a3ed5"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="215.0" Y="217.0" GraphRef="cb421" RelX="0.0" RelY="1.0" /> <Point X="216.0" Y="248.0" GraphRef="ac6ed" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9610442" /> </Interaction> <Interaction GraphId="a45c8"> <Comment Source="Reactome">A SGTA dimer bound to a tail-anchored (TA) protein binds the BAG6 complex (BAG6:GET4:UBL4A) via UBL4A (Winnefeld et al. 2006, Chartron et al. 2012, Xu et al. 2012, Leznicki et al. 2013, Darby et al. 2014, Kuwabara et al. 2015, Mock et al. 2015, Shao et al. 2017, also inferred from rabbit homologs in Mariappan et al. 2010). At some point, ASNA1 (TRC40) also binds the complex (Shao et al. 2017). SGTA interacts with the ubiquitin-like domain (UBL) of BAG6 and the UBL of UBL4A competes with BAG6 for binding to SGTA (Chartron et al. 2012, Xu et al. 2012, Leznicki et al. 2013, Darby et al. 2014, Shao et al. 2017).</Comment> <BiopaxRef>d58</BiopaxRef> <BiopaxRef>aa1</BiopaxRef> <BiopaxRef>abf</BiopaxRef> <BiopaxRef>c8c</BiopaxRef> <BiopaxRef>deb</BiopaxRef> <BiopaxRef>cc3</BiopaxRef> <BiopaxRef>f82</BiopaxRef> <BiopaxRef>dd2</BiopaxRef> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="216.0" Y="248.0" /> <Point X="217.0" Y="294.0" /> <Anchor Position="0.0" Shape="None" GraphId="ac6ed" /> <Anchor Position="0.99" Shape="None" GraphId="e2398" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9610442" /> </Interaction> <Interaction GraphId="a5baa"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="337.0" Y="33.0" GraphRef="f8ea0" RelX="-1.0" RelY="-0.04" /> <Point X="212.0" Y="106.0" GraphRef="eba46" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609921" /> </Interaction> <Interaction GraphId="a7fce"> <Comment Source="Reactome">Membrane proteins are not delivered to membranes with complete efficiency and a portion can end up mislocalized to the cytosol. SGTA binds the hydrophobic regions of such mislocalized proteins that are exposed to the cytosol (Leznicki and High 2012, Xu et al. 2012, Wunderly et al. 2014, Shao et al. 2017). Client proteins of SGTA include both tail-anchored proteins destined for insertion into the endoplasmic reticulum membrane (Leznicki et al. 2013) and inappropriately localized proteins such as cytosolic PRNP (PrP) (Leznicki and High 2012), which is normally located in the plasma membrane. Mislocalized proteins bound by SGTA can be routed to the proteasome via ubiquitination by the BAG6 complex (BAG6:GET4:UBL4A) (Shao et al. 2017).</Comment> <BiopaxRef>aa1</BiopaxRef> <BiopaxRef>c71</BiopaxRef> <BiopaxRef>c8c</BiopaxRef> <BiopaxRef>a36</BiopaxRef> <BiopaxRef>d58</BiopaxRef> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="609.0" Y="110.0" /> <Point X="609.0" Y="154.0" /> <Anchor Position="0.0" Shape="None" GraphId="dd103" /> <Anchor Position="0.99" Shape="None" GraphId="c1b8c" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9617595" /> </Interaction> <Interaction GraphId="a8662"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="410.0" Y="515.0" GraphRef="ebdbd" RelX="0.9884393063583815" RelY="0.0" /> <Point X="443.0" Y="514.0" GraphRef="c9ff4" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609917" /> </Interaction> <Interaction GraphId="b28ef"> <Comment Source="Reactome">A tail-anchored protein bound to ASNA1 (TRC40) (Stefanovic and Hegde 2007, Favaloro et al. 2008, Favaloro et al. 2010) docked at the WRB:CAMLG complex (also known as the WRB:CAML complex) located in the endoplasmic reticulum membrane is released into the ER membrane in an incompletely characterized reaction (Vilardi et al. 2011, Yamamoto and Sakisaka 2012, Vilardi et al. 2014, Colombo et al. 2016, Pfaff et al. 2016, Vogl et al. 2016). Experiments with yeast homologs (GET1 is the homologue of WRB and GET2 is the homologue of CAMLG) indicate that docking of ASNA1:ADP with WRB:CAMLG results in a conformational change in ASNA1 that causes release of the tail-anchored protein to WRB:CAMLG and then into the membrane (inferred from yeast homologs), ADP dissociates from ASNA1 at some point during the reaction. Exchange of ADP for ATP by ASNA1 then recycles ASNA1 back into the cytosol (inferred from yeast homologs).</Comment> <BiopaxRef>b0c</BiopaxRef> <BiopaxRef>f27</BiopaxRef> <BiopaxRef>ae4</BiopaxRef> <BiopaxRef>f45</BiopaxRef> <BiopaxRef>e13</BiopaxRef> <BiopaxRef>e62</BiopaxRef> <BiopaxRef>c1b</BiopaxRef> <BiopaxRef>fe5</BiopaxRef> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="443.0" Y="514.0" /> <Point X="474.0" Y="513.0" /> <Anchor Position="0.0" Shape="None" GraphId="c9ff4" /> <Anchor Position="0.99" Shape="None" GraphId="bce5a" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609917" /> </Interaction> <Interaction GraphId="b7ca6"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="643.0" Y="68.0" GraphRef="e4faa" RelX="0.0" RelY="0.9130434782608695" /> <Point X="609.0" Y="110.0" GraphRef="dd103" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9617595" /> </Interaction> <Interaction GraphId="ba560"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="217.0" Y="294.0" GraphRef="e2398" RelX="0.009999999999990905" RelY="0.45999999999997954" /> <Point X="218.0" Y="297.0" GraphRef="a640b" RelX="0.0" RelY="-1.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9610442" /> </Interaction> <Interaction GraphId="c28a8"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="609.0" Y="185.0" GraphRef="d6cd0" RelX="-0.003389830508474576" RelY="0.9310344827586207" /> <Point X="611.0" Y="238.0" GraphRef="af8a7" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9617596" /> </Interaction> <Interaction GraphId="c35c2"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="241.0" Y="61.0" GraphRef="ec210" RelX="0.9880239520958084" RelY="-0.034482758620689655" /> <Point X="212.0" Y="106.0" GraphRef="eba46" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609921" /> </Interaction> <Interaction GraphId="c3812"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="609.0" Y="154.0" GraphRef="c1b8c" RelX="0.0" RelY="0.4399999999999977" /> <Point X="609.0" Y="157.0" GraphRef="d6cd0" RelX="-0.003389830508474576" RelY="-1.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9617595" /> </Interaction> <Interaction GraphId="c72ec"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="474.0" Y="513.0" GraphRef="bce5a" RelX="0.3100000000000023" RelY="-0.009999999999990905" /> <Point X="507.0" Y="462.0" GraphRef="e7958" RelX="-1.0" RelY="0.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609917" /> </Interaction> <Interaction GraphId="c7697"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="210.0" Y="456.0" GraphRef="ecec4" RelX="-0.0070921985815602835" RelY="1.0" /> <Point X="224.0" Y="476.0" GraphRef="b65d2" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609899" /> </Interaction> <Interaction GraphId="c9fb0"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="216.0" Y="393.0" GraphRef="e0b74" RelX="0.0" RelY="0.5500000000000114" /> <Point X="307.0" Y="258.0" GraphRef="e9e01" RelX="-1.0" RelY="0.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609860" /> </Interaction> <Interaction GraphId="cafc3"> <Comment Source="Reactome">SGTA is believed to initially bind exposed hydrophobic regions of proteins (client proteins) in the cytosol and then associate with the BAG6 complex (BAG6:GET4:UBL4A) and ASNA1 (Mariappan et al. 2010, Hessa et al. 2011, Leznicki et al. 2012, Wunderly et al. 2014, Shao et al. 2017). If the client protein is not efficiently transferred to ASNA1 then the client protein is transferred to BAG6 (Shao et al. 2017), which has been demonstrated to bind hydrophobic regions of proteins and appears to prevent their aggregation prior to proteasomal degradation (Wang et al. 2011). The efficiency of transfer of a client protein to ASNA1 appears to be determined by the sequence of the hydrophobic domain (Hessa et al. 2011). SGTA is not a stable component of the BAG6 complex (Marriapan et al. 2010) and SGTA and RNF126 compete for the same binding site on BAG6 (Krysztofinska et al. 2016).</Comment> <BiopaxRef>c71</BiopaxRef> <BiopaxRef>a36</BiopaxRef> <BiopaxRef>aa1</BiopaxRef> <BiopaxRef>fe9</BiopaxRef> <BiopaxRef>b69</BiopaxRef> <BiopaxRef>e5e</BiopaxRef> <BiopaxRef>ad5</BiopaxRef> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="611.0" Y="238.0" /> <Point X="620.0" Y="293.0" /> <Anchor Position="0.0" Shape="None" GraphId="af8a7" /> <Anchor Position="0.99" Shape="None" GraphId="afc1f" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9617596" /> </Interaction> <Interaction GraphId="cc532"> <Comment Source="Reactome">A tail-anchored protein bound to ASNA1 dimer (TRC40 dimer) in the cytosol (Stefanovic and Hegde 2007, Favaloro et al. 2008, Favaloro et al. 2010) docks at the WRB:CAMLG complex located in the endoplasmic reticulum membrane (Vilardi et al. 2011, Yamamoto and Sakisaka 2012, Vilardi et al. 2014, Colombo et al. 2016, Pfaff et al. 2016, Vogl et al. 2016). The cytoplasmic coiled coil domain of WRB and the cytoplasmic N-terminal domain of CAMLG compete for binding to ASNA1 (Vilardi et al. 2011, Yamamoto and Sakisaka 2012). As inferred from yeast homologs, ASNA1 hydrolyzes bound ATP at some point, resulting in a change of conformation of ASNA1. This may transfer the tail-anchored protein to WRB:CAMLG, which functions as an insertase.</Comment> <BiopaxRef>b0c</BiopaxRef> <BiopaxRef>f27</BiopaxRef> <BiopaxRef>ae4</BiopaxRef> <BiopaxRef>f45</BiopaxRef> <BiopaxRef>e13</BiopaxRef> <BiopaxRef>e62</BiopaxRef> <BiopaxRef>c1b</BiopaxRef> <BiopaxRef>fe5</BiopaxRef> <BiopaxRef>a98</BiopaxRef> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="224.0" Y="476.0" /> <Point X="280.0" Y="493.0" /> <Anchor Position="0.0" Shape="None" GraphId="b65d2" /> <Anchor Position="0.99" Shape="None" GraphId="b7df7" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609899" /> </Interaction> <Interaction GraphId="ce0d7"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="307.0" Y="258.0" GraphRef="e9e01" RelX="-1.0" RelY="0.0" /> <Point X="216.0" Y="248.0" GraphRef="ac6ed" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9610442" /> </Interaction> <Interaction GraphId="cf06f"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="620.0" Y="293.0" GraphRef="afc1f" RelX="0.09000000000003183" RelY="0.5500000000000114" /> <Point X="626.0" Y="296.0" GraphRef="e62e9" RelX="-0.004081632653061225" RelY="-1.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9617596" /> </Interaction> <Interaction GraphId="d35b0"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="412.0" Y="461.0" GraphRef="a0586" RelX="1.0" RelY="0.0" /> <Point X="443.0" Y="514.0" GraphRef="c9ff4" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609917" /> </Interaction> <Interaction GraphId="d4d25"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="218.0" Y="525.0" GraphRef="d325c" RelX="0.978494623655914" RelY="0.0" /> <Point X="224.0" Y="476.0" GraphRef="b65d2" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609899" /> </Interaction> <Interaction GraphId="d5176"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="474.0" Y="513.0" GraphRef="bce5a" RelX="0.3100000000000023" RelY="-0.009999999999990905" /> <Point X="218.0" Y="525.0" GraphRef="d325c" RelX="0.978494623655914" RelY="0.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609917" /> </Interaction> <Interaction GraphId="d88e6"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="216.0" Y="393.0" GraphRef="e0b74" RelX="0.0" RelY="0.5500000000000114" /> <Point X="210.0" Y="418.0" GraphRef="ecec4" RelX="-0.0070921985815602835" RelY="-1.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609860" /> </Interaction> <Interaction GraphId="d9402"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="280.0" Y="493.0" GraphRef="b7df7" RelX="0.5600000000000023" RelY="0.17000000000001592" /> <Point X="324.0" Y="496.0" GraphRef="ebdbd" RelX="-0.005780346820809248" RelY="-1.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609899" /> </Interaction> <Interaction GraphId="d9a9e"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="218.0" Y="335.0" GraphRef="a640b" RelX="0.0" RelY="1.0" /> <Point X="216.0" Y="338.0" GraphRef="e2619" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609860" /> </Interaction> <Interaction GraphId="db327"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="474.0" Y="513.0" GraphRef="bce5a" RelX="0.3100000000000023" RelY="-0.009999999999990905" /> <Point X="497.0" Y="233.0" GraphRef="f418f" RelX="-0.007518796992481203" RelY="1.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609917" /> </Interaction> <Interaction GraphId="ddb92"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="214.0" Y="188.0" GraphRef="bacb2" RelX="0.020000000000010232" RelY="0.8199999999999932" /> <Point X="215.0" Y="191.0" GraphRef="cb421" RelX="0.0" RelY="-1.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609921" /> </Interaction> <Interaction GraphId="ddb93"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="189.0" Y="360.0" GraphRef="d66b2" RelX="1.0" RelY="0.0" /> <Point X="215.0" Y="361.0" GraphRef="eae67" RelX="-1.0" RelY="-4.5" ArrowHead="mim-catalysis" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609860" /> </Interaction> <Interaction GraphId="e0e98"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="439.0" Y="33.0" GraphRef="f8ea0" RelX="0.9805825242718447" RelY="-0.04" /> <Point X="609.0" Y="110.0" GraphRef="dd103" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9617595" /> </Interaction> <Interaction GraphId="e2819"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="216.0" Y="393.0" GraphRef="e0b74" RelX="0.0" RelY="0.5500000000000114" /> <Point X="337.0" Y="33.0" GraphRef="f8ea0" RelX="-1.0" RelY="-0.04" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609860" /> </Interaction> <Interaction GraphId="e3bb6"> <Comment Source="Reactome">A properly folded tail-anchored (TA) protein is transferred from SGTA to ASNA1 via the BAG6 complex (BAG6:UBL4A:GET4 also known at BAT3:GET5:TRC35) (Marriapan et al. 2010, Leznicki et al. 2011, Mock et al. 2015, Shao et al. 2017, Guna et al. 2018). The TA protein bound to ASNA1 is then presumed to dissociate from the BAG6 complex (inferred from yeast homologs). At some point during or shortly after dissociation, ASNA1 hydrolyzes bound ATP to yield ADP (inferred from yeast homologs).&lt;br&gt;Improperly folded proteins and improperly localized hydrophobic proteins are transferred from SGTA to BAG6 rather than to ASNA1 (Shao et al. 2017). BAG6 then recruits RNF126 and facilitates the ubiquitination and consequent degradation of the defective protein. The selection of transferring a substrate protein from SGTA to ASNA1 or to BAG6 thereby acts as a "molecular triage". Proteins with less hydrophobic tail-anchors appear to be inserted into the endoplasmic reticulum membrane by a separate system comprising calmodulin and the ER membrane protein complex (Guna et al. 2018).</Comment> <BiopaxRef>ce2</BiopaxRef> <BiopaxRef>c19</BiopaxRef> <BiopaxRef>aa1</BiopaxRef> <BiopaxRef>dd2</BiopaxRef> <BiopaxRef>e5e</BiopaxRef> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="216.0" Y="338.0" /> <Point X="216.0" Y="393.0" /> <Anchor Position="0.0" Shape="None" GraphId="e2619" /> <Anchor Position="0.99" Shape="None" GraphId="e0b74" /> <Anchor Position="0.5" Shape="None" GraphId="eae67" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609860" /> </Interaction> <Interaction GraphId="ee2e0"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="431.0" Y="221.0" GraphRef="f418f" RelX="-1.0" RelY="0.0" /> <Point X="216.0" Y="248.0" GraphRef="ac6ed" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9610442" /> </Interaction> <Interaction GraphId="ef5e8"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="439.0" Y="258.0" GraphRef="e9e01" RelX="1.0" RelY="0.0" /> <Point X="611.0" Y="238.0" GraphRef="af8a7" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9617596" /> </Interaction> <Interaction GraphId="f08c2"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="216.0" Y="393.0" GraphRef="e0b74" RelX="0.0" RelY="0.5500000000000114" /> <Point X="280.0" Y="395.0" GraphRef="e98a4" RelX="-1.0" RelY="0.0" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609860" /> </Interaction> <Interaction GraphId="f5ab0"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="474.0" Y="513.0" GraphRef="bce5a" RelX="0.3100000000000023" RelY="-0.009999999999990905" /> <Point X="516.0" Y="527.0" GraphRef="f057a" RelX="-1.0" RelY="-0.02857142857142857" ArrowHead="Arrow" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609917" /> </Interaction> <Interaction GraphId="fbac2"> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="563.0" Y="221.0" GraphRef="f418f" RelX="0.9849624060150376" RelY="0.0" /> <Point X="611.0" Y="238.0" GraphRef="af8a7" RelX="0.0" RelY="0.0" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9617596" /> </Interaction> <Interaction GraphId="fe514"> <Comment Source="Reactome">The C-terminal domains of a SGTA dimer bind the C-terminal hydrophobic transmembrane domain (TMD) of a tail-anchored (TA) protein shortly after it emerges from the ribosome (Liou and Wang 2005, Leznicki et al. 2011, Wunderley et al. 2014, Shao et al. 2017). Binding of SGTA shields the TMD of the TA protein and stabilizes the TA protein by preventing it from being targeted for degradation (Leznicki and High 2012).</Comment> <BiopaxRef>aa1</BiopaxRef> <BiopaxRef>a36</BiopaxRef> <BiopaxRef>c19</BiopaxRef> <BiopaxRef>c1e</BiopaxRef> <BiopaxRef>c71</BiopaxRef> <Graphics ConnectorType="Segmented" ZOrder="12288" LineThickness="1.0"> <Point X="212.0" Y="106.0" /> <Point X="214.0" Y="188.0" /> <Anchor Position="0.0" Shape="None" GraphId="eba46" /> <Anchor Position="0.99" Shape="None" GraphId="bacb2" /> </Graphics> <Xref Database="Reactome" ID="R-HSA-9609921" /> </Interaction> <Label TextLabel="cytosol" GraphId="a0332" GroupRef="bb225"> <Graphics CenterX="52.0" CenterY="599.5" Width="64.0" Height="33.0" ZOrder="28672" FillColor="ffffff" FontSize="10" Valign="Middle" /> </Label> <Label TextLabel="endoplasmic reticulum membrane" GraphId="a926d" GroupRef="d04e7"> <Graphics CenterX="328.0" CenterY="567.5" Width="232.0" Height="33.0" ZOrder="28672" FillColor="ffffff" FontSize="10" Valign="Middle" /> </Label> <Shape GraphId="b47e9" GroupRef="bb225"> <Graphics CenterX="389.0" CenterY="317.0" Width="766.0" Height="626.0" ZOrder="16384" FontSize="10" Valign="Middle" ShapeType="RoundedRectangle" LineThickness="3.0" Color="c0c0c0" Rotation="0.0" /> </Shape> <Shape GraphId="f3659" GroupRef="d04e7"> <Graphics CenterX="386.0" CenterY="548.0" Width="610.0" Height="78.0" ZOrder="16384" FontSize="10" Valign="Middle" ShapeType="RoundedRectangle" LineThickness="3.0" Color="c0c0c0" Rotation="0.0" /> </Shape> <Group GroupId="be72f" GraphId="a0d25" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9609864" /> </Group> <Group GroupId="bb225" GraphId="a507b" Style="Group" /> <Group GroupId="a889e" GraphId="ac0e1" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9609912" /> </Group> <Group GroupId="ac236" GraphId="afd54" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9610464" /> </Group> <Group GroupId="c5451" GraphId="bdc7b" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9617602" /> </Group> <Group GroupId="d04e7" GraphId="be02e" Style="Group" /> <Group GroupId="dc45b" GraphId="c562c" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9610465" /> </Group> <Group GroupId="f29ea" GraphId="c5b31" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9609895" /> </Group> <Group GroupId="dd7bc" GraphId="c72ed" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9610487" /> </Group> <Group GroupId="cb890" GraphId="cb965" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9609923" /> </Group> <Group GroupId="dae8b" GraphId="cd2de" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9609902" /> </Group> <Group GroupId="b74ae" GraphId="cd50c"> <Attribute Key="complex_id" Value="R-HSA-9609909" /> </Group> <Group GroupId="c82bc" GraphId="cd7e5" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9617598" /> </Group> <Group GroupId="f44ad" GraphId="d4006" Style="Complex"> <Attribute Key="complex_id" Value="R-HSA-9628396" /> </Group> <Group GroupId="fb484" GraphId="d7348"> <Attribute Key="complex_id" Value="R-HSA-9609893" /> </Group> <Group GroupId="d8390" GraphId="f4ac6"> <Attribute Key="complex_id" Value="R-HSA-9617594" /> </Group> <InfoBox CenterX="0.0" CenterY="0.0" /> <Biopax> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="c1e"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">15708368</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Small glutamine-rich tetratricopeptide repeat-containing protein is composed of three structural units with distinct functions.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Liou ST, Wang C.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="ae4"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">20375064</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Asna1/TRC40-mediated membrane insertion of tail-anchored proteins.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Favaloro V, Vilardi F, Schlecht R, Mayer MP, Dobberstein B.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="fe9"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">27193484</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Structural and functional insights into the E3 ligase, RNF126.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Krysztofinska EM, Martínez-Lumbreras S, Thapaliya A, Evans NJ, High S, Isaacson RL.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="a00"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">24981174</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Rodrigo-Brenni MC, Gutierrez E, Hegde RS.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="efb"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">29042515</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Structural basis for regulation of the nucleo-cytoplasmic distribution of Bag6 by TRC35.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Mock JY, Xu Y, Ye Y, Clemons WM.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="e13"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">21444755</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Vilardi F, Lorenz H, Dobberstein B.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="d11"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">29689233</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Transmembrane Domain Recognition during Membrane Protein Biogenesis and Quality Control.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Guna A, Hegde RS.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="a98"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">24392163</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">WRB and CAML are necessary and sufficient to mediate tail-anchored protein targeting to the ER membrane.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Vilardi F, Stephan M, Clancy A, Janshoff A, Schwappach B.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="df8"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">11306053</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Fatty aldehyde dehydrogenase: genomic structure, expression and mutation analysis in Sjögren-Larsson syndrome.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Rizzo WB, Lin Z, Carney G.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="c71"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">23129660</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SGTA antagonizes BAG6-mediated protein triage.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Leznicki P, High S.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="b0c"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">17382883</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Identification of a targeting factor for posttranslational membrane protein insertion into the ER.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Stefanovic S, Hegde RS.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="d48"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">28992441</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Mechanisms of Tail-Anchored Membrane Protein Targeting and Insertion.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Chio US, Cho H, Shan SO.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="e91"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">27284044</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">On the road to nowhere: cross-talk between post-translational protein targeting and cytosolic quality control.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Casson J, McKenna M, High S.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="c9e"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">25043001</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">The Get1/2 transmembrane complex is an endoplasmic-reticulum membrane protein insertase.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Wang F, Chan C, Weir NR, Denic V.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="aa1"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">28104892</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Mechanistic basis for a molecular triage reaction.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Shao S, Rodrigo-Brenni MC, Kivlen MH, Hegde RS.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="deb"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">23142665</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Structures of the Sgt2/SGTA dimerization domain with the Get5/UBL4A UBL domain reveal an interaction that forms a conserved dynamic interface.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Chartron JW, VanderVelde DG, Clemons WM.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="f27"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">18477612</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Distinct targeting pathways for the membrane insertion of tail-anchored (TA) proteins.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Favaloro V, Spasic M, Schwappach B, Dobberstein B.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="e78"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">29996828</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Structural complexity of the co-chaperone SGTA: a conserved C-terminal region is implicated in dimerization and substrate quality control.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Martínez-Lumbreras S, Krysztofinska EM, Thapaliya A, Spilotros A, Matak-Vinkovic D, Salvadori E, Roboti P, Nyathi Y, Muench JH, Roessler MM, Svergun DI, High S, Isaacson RL.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="c8c"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">23246001</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SGTA recognizes a noncanonical ubiquitin-like domain in the Bag6-Ubl4A-Trc35 complex to promote endoplasmic reticulum-associated degradation.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Xu Y, Cai M, Yang Y, Huang L, Ye Y.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="dd2"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">25535373</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Bag6 complex contains a minimal tail-anchor-targeting module and a mock BAG domain.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Mock JY, Chartron JW, Zaslaver M, Xu Y, Ye Y, Clemons WM.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="cc3"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">16777091</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Human SGT interacts with Bag-6/Bat-3/Scythe and cells with reduced levels of either protein display persistence of few misaligned chromosomes and mitotic arrest.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Winnefeld M, Grewenig A, Schnölzer M, Spring H, Knoch TA, Gan EC, Rommelaere J, Cziepluch C.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="d58"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">23533635</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">The association of BAG6 with SGTA and tail-anchored proteins.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Leznicki P, Roebuck QP, Wunderley L, Clancy A, Krysztofinska EM, Isaacson RL, Warwicker J, Schwappach B, High S.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="fe5"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">27458190</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Tryptophan-rich basic protein (WRB) mediates insertion of the tail-anchored protein otoferlin and is required for hair cell exocytosis and hearing.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Vogl C, Panou I, Yamanbaeva G, Wichmann C, Mangosing SJ, Vilardi F, Indzhykulian AA, Pangršič T, Santarelli R, Rodriguez-Ballesteros M, Weber T, Jung S, Cardenas E, Wu X, Wojcik SM, Kwan KY, Del Castillo I, Schwappach B, Strenzke N, Corey DP, Lin SY, Moser T.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="ad5"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">21636303</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A ubiquitin ligase-associated chaperone holdase maintains polypeptides in soluble states for proteasome degradation.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Wang Q, Liu Y, Soetandyo N, Baek K, Hegde R, Ye Y.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="a36"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">25179605</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SGTA regulates the cytosolic quality control of hydrophobic substrates.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Wunderley L, Leznicki P, Payapilly A, High S.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="c1b"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">26675233</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Pfaff J, Rivera Monroy J, Jamieson C, Rajanala K, Vilardi F, Schwappach B, Kehlenbach RH.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="a18"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">29021347</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Multiple pathways facilitate the biogenesis of mammalian tail-anchored proteins.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Casson J, McKenna M, Haßdenteufel S, Aviram N, Zimmerman R, High S.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="f45"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">23041287</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Yamamoto Y, Sakisaka T.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="e5e"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">20676083</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A ribosome-associating factor chaperones tail-anchored membrane proteins.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Mariappan M, Li X, Stefanovic S, Sharma A, Mateja A, Keenan RJ, Hegde RS.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="ce2"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">29242231</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">The ER membrane protein complex is a transmembrane domain insertase.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Guna A, Volkmar N, Christianson JC, Hegde RS.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="e62"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">27226539</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Tail-anchored Protein Insertion in Mammals: FUNCTION AND RECIPROCAL INTERACTIONS OF THE TWO SUBUNITS OF THE TRC40 RECEPTOR.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Colombo SF, Cardani S, Maroli A, Vitiello A, Soffientini P, Crespi A, Bram RF, Benfante R, Borgese N.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="cc8"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">23417671</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Bag6/Bat3/Scythe: a novel chaperone activity with diverse regulatory functions in protein biogenesis and degradation.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Lee JG, Ye Y.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="b69"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">21743475</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Protein targeting and degradation are coupled for elimination of mislocalized proteins.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Hessa T, Sharma A, Mariappan M, Eshleman HD, Gutierrez E, Hegde RS.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="af2"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">20646998</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Targeting pathways of C-tail-anchored proteins.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Borgese N, Fasana E.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="c19"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">21466504</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A biochemical analysis of the constraints of tail-anchored protein biogenesis.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Leznicki P, Warwicker J, High S.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="f47"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">27905431</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">The SND proteins constitute an alternative targeting route to the endoplasmic reticulum.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Aviram N, Ast T, Costa EA, Arakel EC, Chuartzman SG, Jan CH, Haßdenteufel S, Dudek J, Jung M, Schorr S, Zimmermann R, Schwappach B, Weissman JS, Schuldiner M.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="abf"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">25415308</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Solution structure of the SGTA dimerisation domain and investigation of its interactions with the ubiquitin-like domains of BAG6 and UBL4A.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Darby JF, Krysztofinska EM, Simpson PJ, Simon AC, Leznicki P, Sriskandarajah N, Bishop DS, Hale LR, Alfano C, Conte MR, Martínez-Lumbreras S, Thapaliya A, High S, Isaacson RL.</bp:AUTHORS> </bp:PublicationXref> <bp:PublicationXref xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:id="f82"> <bp:ID rdf:datatype="http://www.w3.org/2001/XMLSchema#string">25713138</bp:ID> <bp:DB rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PubMed</bp:DB> <bp:TITLE rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Structure of a BAG6 (Bcl-2-associated athanogene 6)-Ubl4a (ubiquitin-like protein 4a) complex reveals a novel binding interface that functions in tail-anchored protein biogenesis.</bp:TITLE> <bp:AUTHORS rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Kuwabara N, Minami R, Yokota N, Matsumoto H, Senda T, Kawahara H, Kato R.</bp:AUTHORS> </bp:PublicationXref> <bp:openControlledVocabulary xmlns:bp="http://www.biopax.org/release/biopax-level3.owl#"> <bp:TERM xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:datatype="http://www.w3.org/2001/XMLSchema#string">protein secretory pathway</bp:TERM> <bp:ID xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PW:0000280</bp:ID> <bp:Ontology xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Pathway Ontology</bp:Ontology> </bp:openControlledVocabulary> </Biopax> </Pathway>

• Template:AuthorInfo
• Template:CheckAvailable (protected)
• Template:PathwayPage:History
• Template:PathwayPage:Xrefs

Return to Insertion of tail-anchored proteins into the endoplasmic reticulum membrane (Homo sapiens).