Nucleotide Excision Repair (Homo sapiens)

From WikiPathways

Revision as of 08:38, 30 April 2014 by ReactomeTeam (Talk | contribs)
Jump to: navigation, search
4, 5, 9173, 8771RPA heterotrimer TFIIH RNA Polymerase II holoenzyme complex XPCHR23Bdamaged DNA complex TFIIH TFIIH ERCC1XPF complex Transcription-coupled ERCC1XPF complex XPCHR23B complex RNA Polymerase II holoenzyme complex nucleoplasmActive Pol II complex with repaired DNA templatemRNA hybrid PCNA homotrimer TFIIH Stalled Pol II complex with damaged DNA hybrid DNA polymerase epsilon TFIIH CAK CAK DNA Polymerase delta tetramer DNA Polymerase delta tetramer RPA heterotrimer RPA heterotrimer pre-incision complex in GG-NER XPCHR23B complex CAK Incision complex with 3'-incised damaged DNA pre-incision complex with open DNA bubble XPCHR23B complex XPCHR23B complex Active Pol II transcription complex with damaged DNA hybrid CAK Stalled Pol II in TC-NER RNA Polymerase II holoenzyme complex incision complex for GG-NER XPCHR23B complex RPA heterotrimer ERCC1XPF complex XPCHR23B complex RNA Polymerase II holoenzyme complex CAK TFIIH RPA heterotrimer pre-incision complex with open DNA bubble CAK RNA Polymerase II holoenzyme complex XPCHR23Bdamaged DNA complex RFC Heteropentamer ERCC1XPF complex MNAT1 excised DNA fragment with lesionERCC5 CCNH POLR2H GTF2H1ERCC2 RAD23B LIG1XAB2ERCC5 PCNA homotrimerERCC3 POLR2K POLR2E POLR2E dNTPERCC8 POLR2K XAB2 CCNH dNTPRNA Polymerase II holoenzyme complex POLR2L p-S2,S5-POLR2A ERCC2 POLD1 ERCC3 POLR2B POLD3 POLR2K POLR2I DDB1GTF2H3 ERCC4 XPCXPARFC1 incision complex for GG-NERCDK7 XPC POLR2J POLR2DRPA1 XPARFC3 RPA2 GTF2H3 POLR2Fincised DNA without lesionActive Pol II complex with repaired DNA templatemRNA hybridRPA3 DDB1 ERCC3 newly synthesized DNA fragmentDDB2 incised DNA without lesionPOLR2J damaged DNA substratenascent mRNA hybrid with dual incisionsERCC2 XPAGTF2H2 GTF2H4 RFC4 Repaired double-stranded DNAPOLR2G POLR2H RPA3 POLD4 RFC5 GTF2H1POLR2FERCC1 dNTPPOLR2I POLR2FPOLE POLD4 CDK7 POLR2B POLR2I ERCC5POLR2E p-S2,S5-POLR2A MNAT1 GTF2H2 RPA heterotrimerXPCHR23Bdamaged DNA complexincised DNA without lesionPOLR2H POLR2C POLR2H RAD23B ERCC5 DDB1 RPA3 DDB2 damaged DNA substratenascent mRNA hybridERCC5 POLR2G POLR2L DDB1 ERCC4 POLD3 RPA1 CCNH ERCC5 dNTPERCC1 ERCC3 GTF2H2 POLD1 ERCC1POLR2FERCC1 POLR2J ERCC2 POLR2C MNAT1 POLR2H POLR2DGTF2H3 POLR2K GTF2H3 MNAT1 RPA1 Active Pol II transcription complex with damaged DNA hybridGTF2H4 ERCC4 RAD23B CCNH DDB1 RAD23B RAD23BRPA2 ERCC3 Incision complex with 3'-incised damaged DNAPOLR2G RPA2 POLR2C GTF2H1POLR2B GTF2H4 RFC HeteropentamerGTF2H2 GTF2H2 POLR2FRPA1 ERCC2 POLD2 GTF2H4 Stalled Pol II in TC-NERGTF2H1ERCC1 GTF2H3 POLR2L GTF2H1RPA1 POLE2 XPARPA3 ERCC3 CDK7 MNAT1 XPAPOLR2B POLR2Dp-S2,S5-POLR2A POLR2E ERCC2 CCNH POLR2E p-S2,S5-POLR2A TCEA1 DDB2 DDB2POLR2J POLR2I POLR2C newly synthesized DNA fragmentTFIIHRAD23B POLR2DTranscription-coupled newly synthesized DNA fragmentPOLR2C newly synthesized DNA fragmentpre-incision complex in GG-NERXPC POLR2G XPC GTF2H4 POLR2J DDB2 ERCC6 POLR2DERCC8PCNA RPA3 GTF2H2 POLR2I p-S2,S5-POLR2A Stalled Pol II complex with damaged DNA hybriddamaged DNA substratenascent mRNA hybrid with 3' incisionTCEA1XPC newly synthesized DNA fragmentPOLR2G GTF2H1pre-incision complex with open DNA bubbleRFC2 GTF2H3 POLR2L damaged DNA substratenewly synthesized DNA fragmentPOLR2L repaired DNA templatenascent mRNA hybridXPC DNA polymerase epsilonERCC4 GTF2H4 XPCHR23B complexCDK7 ERCC4ERCC6CDK7 DNA Polymerase delta tetramerCCNH DNA Polymerase delta tetramerMNAT1 RAD23B POLR2B RPA2 ERCC1XPF complexXPC POLR2K RPA2 POLD2 incised DNA without lesionCDK7 662666


Description

NER was first described in the model organism E. coli in the early 1960s as a process whereby bulky base damage is enzymatically removed from DNA, facilitating the recovery of DNA synthesis and cell survival. Deficient NER processes have been identified from the cells of cancer-prone patients with different variants of xeroderma pigmentosum (XP), trichothiodystrophy (TTD), and Cockayne’s syndrome. These XP cells exhibited an ultraviolet radiation hypersensitivity leading to a hypermutability response to UV, offering a direct connection between deficient NER, increased mutations, and cancer. While the NER pathway in prokaryotes is unique, the pathway utilized in yeast and higher eukaryotes is highly conserved and includes a variety of proteins that interact to form complexes.
NER is involved in the repair of bulky adducts in DNA, such as UV-induced photo lesions [of both 6-4 photoproducts (6-4 pps) and cyclobutane pyrimidine dimer (CPDs)], intrastrand cross-links, large chemical adducts formed from exposure to aflatoxin, benzopyrene and other genotoxic agents. Specific proteins have been identified that participate in base damage recognition, cleavage of the damaged strand on both sides of the lesion, excision of the oligonucleotide bearing the lesion, and accessory proteins necessary for efficient function. Polymerization and ligation restore the strand to its original state. NER consists of two related pathways called global genomic repair (GG-NER) and transcription-coupled NER (TC-NER). The pathways differ in the way in which DNA damage is initially recognized, but the majority of the participating molecules are shared between these two branches of NER". GG-NER is considered to be transcription-independent, removing lesions from non-transcribed regions of genome in addition to non-transcribed strands of transcribed regions. The preferential repair of UV-induced damage in transcribed strands of active genes is known as Transcription-coupled NER (TC-NER).
Several of the proteins involved in NER are key components of the basal transcription complex TFIIH. NER proteins have also been shown to interact with the 19S regulatory subunit of the proteasome, suggesting a role in cellular regulation signal pathways. The establishment of mutant mouse models for NER genes and other DNA repair-related genes have been useful in demonstrating the associations between NER defects and cancer.

Original Pathway at Reactome: http://www.reactome.org/PathwayBrowser/#DB=gk_current&FOCUS_SPECIES_ID=48887&FOCUS_PATHWAY_ID=73885

Try the New WikiPathways

View approved pathways at the new wikipathways.org.

Quality Tags

Ontology Terms

 

Bibliography

View all...
  1. Wang QE, Zhu Q, Wani G, Chen J, Wani AA.; ''UV radiation-induced XPC translocation within chromatin is mediated by damaged-DNA binding protein, DDB2.''; PubMed Europe PMC Scholia
  2. Robu M, Shah RG, Petitclerc N, Brind'Amour J, Kandan-Kulangara F, Shah GM.; ''Role of poly(ADP-ribose) polymerase-1 in the removal of UV-induced DNA lesions by nucleotide excision repair.''; PubMed Europe PMC Scholia
  3. Fousteri M, Vermeulen W, van Zeeland AA, Mullenders LH.; ''Cockayne syndrome A and B proteins differentially regulate recruitment of chromatin remodeling and repair factors to stalled RNA polymerase II in vivo.''; PubMed Europe PMC Scholia
  4. Lee KB, Wang D, Lippard SJ, Sharp PA.; ''Transcription-coupled and DNA damage-dependent ubiquitination of RNA polymerase II in vitro.''; PubMed Europe PMC Scholia
  5. Shiyanov P, Nag A, Raychaudhuri P.; ''Cullin 4A associates with the UV-damaged DNA-binding protein DDB.''; PubMed Europe PMC Scholia
  6. Masutani C, Sugasawa K, Yanagisawa J, Sonoyama T, Ui M, Enomoto T, Takio K, Tanaka K, van der Spek PJ, Bootsma D.; ''Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23.''; PubMed Europe PMC Scholia
  7. Winkler GS, Sugasawa K, Eker AP, de Laat WL, Hoeijmakers JH.; ''Novel functional interactions between nucleotide excision DNA repair proteins influencing the enzymatic activities of TFIIH, XPG, and ERCC1-XPF.''; PubMed Europe PMC Scholia
  8. Wang H, Zhai L, Xu J, Joo HY, Jackson S, Erdjument-Bromage H, Tempst P, Xiong Y, Zhang Y.; ''Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage.''; PubMed Europe PMC Scholia
  9. Ito S, Kuraoka I, Chymkowitch P, Compe E, Takedachi A, Ishigami C, Coin F, Egly JM, Tanaka K.; ''XPG stabilizes TFIIH, allowing transactivation of nuclear receptors: implications for Cockayne syndrome in XP-G/CS patients.''; PubMed Europe PMC Scholia
  10. Birger Y, West KL, Postnikov YV, Lim JH, Furusawa T, Wagner JP, Laufer CS, Kraemer KH, Bustin M.; ''Chromosomal protein HMGN1 enhances the rate of DNA repair in chromatin.''; PubMed Europe PMC Scholia
  11. Coin F, Oksenych V, Mocquet V, Groh S, Blattner C, Egly JM.; ''Nucleotide excision repair driven by the dissociation of CAK from TFIIH.''; PubMed Europe PMC Scholia
  12. Brueckner F, Hennecke U, Carell T, Cramer P.; ''CPD damage recognition by transcribing RNA polymerase II.''; PubMed Europe PMC Scholia
  13. Ziani S, Nagy Z, Alekseev S, Soutoglou E, Egly JM, Coin F.; ''Sequential and ordered assembly of a large DNA repair complex on undamaged chromatin.''; PubMed Europe PMC Scholia
  14. Schultz P, Fribourg S, Poterszman A, Mallouh V, Moras D, Egly JM.; ''Molecular structure of human TFIIH.''; PubMed Europe PMC Scholia
  15. Bregman DB, Halaban R, van Gool AJ, Henning KA, Friedberg EC, Warren SL.; ''UV-induced ubiquitination of RNA polymerase II: a novel modification deficient in Cockayne syndrome cells.''; PubMed Europe PMC Scholia
  16. Furuta T, Ueda T, Aune G, Sarasin A, Kraemer KH, Pommier Y.; ''Transcription-coupled nucleotide excision repair as a determinant of cisplatin sensitivity of human cells.''; PubMed Europe PMC Scholia
  17. Friedberg EC.; ''How nucleotide excision repair protects against cancer.''; PubMed Europe PMC Scholia
  18. Orelli B, McClendon TB, Tsodikov OV, Ellenberger T, Niedernhofer LJ, Schärer OD.; ''The XPA-binding domain of ERCC1 is required for nucleotide excision repair but not other DNA repair pathways.''; PubMed Europe PMC Scholia
  19. Selby CP, Sancar A.; ''Cockayne syndrome group B protein enhances elongation by RNA polymerase II.''; PubMed Europe PMC Scholia
  20. Kuper J, Wolski SC, Michels G, Kisker C.; ''Functional and structural studies of the nucleotide excision repair helicase XPD suggest a polarity for DNA translocation.''; PubMed Europe PMC Scholia
  21. Yokoi M, Masutani C, Maekawa T, Sugasawa K, Ohkuma Y, Hanaoka F.; ''The xeroderma pigmentosum group C protein complex XPC-HR23B plays an important role in the recruitment of transcription factor IIH to damaged DNA.''; PubMed Europe PMC Scholia
  22. Fujiwara Y, Masutani C, Mizukoshi T, Kondo J, Hanaoka F, Iwai S.; ''Characterization of DNA recognition by the human UV-damaged DNA-binding protein.''; PubMed Europe PMC Scholia
  23. Ng JM, Vermeulen W, van der Horst GT, Bergink S, Sugasawa K, Vrieling H, Hoeijmakers JH.; ''A novel regulation mechanism of DNA repair by damage-induced and RAD23-dependent stabilization of xeroderma pigmentosum group C protein.''; PubMed Europe PMC Scholia
  24. Camenisch U, Dip R, Schumacher SB, Schuler B, Naegeli H.; ''Recognition of helical kinks by xeroderma pigmentosum group A protein triggers DNA excision repair.''; PubMed Europe PMC Scholia
  25. Pines A, Vrouwe MG, Marteijn JA, Typas D, Luijsterburg MS, Cansoy M, Hensbergen P, Deelder A, de Groot A, Matsumoto S, Sugasawa K, Thoma N, Vermeulen W, Vrieling H, Mullenders L.; ''PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1.''; PubMed Europe PMC Scholia
  26. Anindya R, Mari PO, Kristensen U, Kool H, Giglia-Mari G, Giglia-Mari G, Mullenders LH, Fousteri M, Vermeulen W, Egly JM, Svejstrup JQ.; ''A ubiquitin-binding domain in Cockayne syndrome B required for transcription-coupled nucleotide excision repair.''; PubMed Europe PMC Scholia
  27. Overmeer RM, Moser J, Volker M, Kool H, Tomkinson AE, van Zeeland AA, Mullenders LH, Fousteri M.; ''Replication protein A safeguards genome integrity by controlling NER incision events.''; PubMed Europe PMC Scholia
  28. Dunand-Sauthier I, Hohl M, Thorel F, Jaquier-Gubler P, Clarkson SG, Schärer OD.; ''The spacer region of XPG mediates recruitment to nucleotide excision repair complexes and determines substrate specificity.''; PubMed Europe PMC Scholia
  29. King BS, Cooper KL, Liu KJ, Hudson LG.; ''Poly(ADP-ribose) contributes to an association between poly(ADP-ribose) polymerase-1 and xeroderma pigmentosum complementation group A in nucleotide excision repair.''; PubMed Europe PMC Scholia
  30. Park CH, Bessho T, Matsunaga T, Sancar A.; ''Purification and characterization of the XPF-ERCC1 complex of human DNA repair excision nuclease.''; PubMed Europe PMC Scholia
  31. Nakatsu Y, Asahina H, Citterio E, Rademakers S, Vermeulen W, Kamiuchi S, Yeo JP, Khaw MC, Saijo M, Kodo N, Matsuda T, Hoeijmakers JH, Tanaka K.; ''XAB2, a novel tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription.''; PubMed Europe PMC Scholia
  32. Dinant C, Ampatziadis-Michailidis G, Lans H, Tresini M, Lagarou A, Grosbart M, Theil AF, van Cappellen WA, Kimura H, Bartek J, Fousteri M, Houtsmuller AB, Vermeulen W, Marteijn JA.; ''Enhanced chromatin dynamics by FACT promotes transcriptional restart after UV-induced DNA damage.''; PubMed Europe PMC Scholia
  33. Conaway RC, Conaway JW.; ''The INO80 chromatin remodeling complex in transcription, replication and repair.''; PubMed Europe PMC Scholia
  34. Mackinnon-Roy C, Stubbert LJ, McKay BC.; ''RNA interference against transcription elongation factor SII does not support its role in transcription-coupled nucleotide excision repair.''; PubMed Europe PMC Scholia
  35. Riedl T, Hanaoka F, Egly JM.; ''The comings and goings of nucleotide excision repair factors on damaged DNA.''; PubMed Europe PMC Scholia
  36. Ikegami T, Kuraoka I, Saijo M, Kodo N, Kyogoku Y, Morikawa K, Tanaka K, Shirakawa M.; ''Solution structure of the DNA- and RPA-binding domain of the human repair factor XPA.''; PubMed Europe PMC Scholia
  37. Christmann M, Tomicic MT, Roos WP, Kaina B.; ''Mechanisms of human DNA repair: an update.''; PubMed Europe PMC Scholia
  38. Min JH, Pavletich NP.; ''Recognition of DNA damage by the Rad4 nucleotide excision repair protein.''; PubMed Europe PMC Scholia
  39. Schwertman P, Lagarou A, Dekkers DH, Raams A, van der Hoek AC, Laffeber C, Hoeijmakers JH, Demmers JA, Fousteri M, Vermeulen W, Marteijn JA.; ''UV-sensitive syndrome protein UVSSA recruits USP7 to regulate transcription-coupled repair.''; PubMed Europe PMC Scholia
  40. Marteijn JA, Lans H, Vermeulen W, Hoeijmakers JH.; ''Understanding nucleotide excision repair and its roles in cancer and ageing.''; PubMed Europe PMC Scholia
  41. Araki M, Masutani C, Takemura M, Uchida A, Sugasawa K, Kondoh J, Ohkuma Y, Hanaoka F.; ''Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair.''; PubMed Europe PMC Scholia
  42. Sollier J, Stork CT, García-Rubio ML, Paulsen RD, Aguilera A, Cimprich KA.; ''Transcription-coupled nucleotide excision repair factors promote R-loop-induced genome instability.''; PubMed Europe PMC Scholia
  43. Mourgues S, Gautier V, Lagarou A, Bordier C, Mourcet A, Slingerland J, Kaddoum L, Coin F, Vermeulen W, Gonzales de Peredo A, Monsarrat B, Mari PO, Giglia-Mari G.; ''ELL, a novel TFIIH partner, is involved in transcription restart after DNA repair.''; PubMed Europe PMC Scholia
  44. Wang QE, Zhu Q, Wani G, El-Mahdy MA, Li J, Wani AA.; ''DNA repair factor XPC is modified by SUMO-1 and ubiquitin following UV irradiation.''; PubMed Europe PMC Scholia
  45. Balajee AS, May A, Dianova I, Bohr VA.; ''Efficient PCNA complex formation is dependent upon both transcription coupled repair and genome overall repair.''; PubMed Europe PMC Scholia
  46. Scrima A, Konícková R, Czyzewski BK, Kawasaki Y, Jeffrey PD, Groisman R, Groisman R, Nakatani Y, Iwai S, Pavletich NP, Thomä NH.; ''Structural basis of UV DNA-damage recognition by the DDB1-DDB2 complex.''; PubMed Europe PMC Scholia
  47. Hanawalt PC, Spivak G.; ''Transcription-coupled DNA repair: two decades of progress and surprises.''; PubMed Europe PMC Scholia
  48. Jiang Y, Wang X, Bao S, Guo R, Johnson DG, Shen X, Li L.; ''INO80 chromatin remodeling complex promotes the removal of UV lesions by the nucleotide excision repair pathway.''; PubMed Europe PMC Scholia
  49. Wakasugi M, Shimizu M, Morioka H, Linn S, Nikaido O, Matsunaga T.; ''Damaged DNA-binding protein DDB stimulates the excision of cyclobutane pyrimidine dimers in vitro in concert with XPA and replication protein A.''; PubMed Europe PMC Scholia
  50. Fei J, Chen J.; ''KIAA1530 protein is recruited by Cockayne syndrome complementation group protein A (CSA) to participate in transcription-coupled repair (TCR).''; PubMed Europe PMC Scholia
  51. Sugasawa K, Akagi J, Nishi R, Iwai S, Hanaoka F.; ''Two-step recognition of DNA damage for mammalian nucleotide excision repair: Directional binding of the XPC complex and DNA strand scanning.''; PubMed Europe PMC Scholia
  52. Tsodikov OV, Ivanov D, Orelli B, Staresincic L, Shoshani I, Oberman R, Schärer OD, Wagner G, Ellenberger T.; ''Structural basis for the recruitment of ERCC1-XPF to nucleotide excision repair complexes by XPA.''; PubMed Europe PMC Scholia
  53. Sarker AH, Tsutakawa SE, Kostek S, Ng C, Shin DS, Peris M, Campeau E, Tainer JA, Nogales E, Cooper PK.; ''Recognition of RNA polymerase II and transcription bubbles by XPG, CSB, and TFIIH: insights for transcription-coupled repair and Cockayne Syndrome.''; PubMed Europe PMC Scholia
  54. Rossignol M, Kolb-Cheynel I, Egly JM.; ''Substrate specificity of the cdk-activating kinase (CAK) is altered upon association with TFIIH.''; PubMed Europe PMC Scholia
  55. Mathieu N, Kaczmarek N, Naegeli H.; ''Strand- and site-specific DNA lesion demarcation by the xeroderma pigmentosum group D helicase.''; PubMed Europe PMC Scholia
  56. Zhang X, Horibata K, Saijo M, Ishigami C, Ukai A, Kanno S, Tahara H, Neilan EG, Honma M, Nohmi T, Yasui A, Tanaka K.; ''Mutations in UVSSA cause UV-sensitive syndrome and destabilize ERCC6 in transcription-coupled DNA repair.''; PubMed Europe PMC Scholia
  57. Poulsen SL, Hansen RK, Wagner SA, van Cuijk L, van Belle GJ, Streicher W, Wikström M, Choudhary C, Houtsmuller AB, Marteijn JA, Bekker-Jensen S, Mailand N.; ''RNF111/Arkadia is a SUMO-targeted ubiquitin ligase that facilitates the DNA damage response.''; PubMed Europe PMC Scholia
  58. Fang L, Wang X, Yamoah K, Chen PL, Pan ZQ, Huang L.; ''Characterization of the human COP9 signalosome complex using affinity purification and mass spectrometry.''; PubMed Europe PMC Scholia
  59. Jawhari A, Lainé JP, Dubaele S, Lamour V, Poterszman A, Coin F, Moras D, Egly JM.; ''p52 Mediates XPB function within the transcription/repair factor TFIIH.''; PubMed Europe PMC Scholia
  60. Epshtein V, Kamarthapu V, McGary K, Svetlov V, Ueberheide B, Proshkin S, Mironov A, Nudler E.; ''UvrD facilitates DNA repair by pulling RNA polymerase backwards.''; PubMed Europe PMC Scholia
  61. Moser J, Kool H, Giakzidis I, Caldecott K, Mullenders LH, Fousteri MI.; ''Sealing of chromosomal DNA nicks during nucleotide excision repair requires XRCC1 and DNA ligase III alpha in a cell-cycle-specific manner.''; PubMed Europe PMC Scholia
  62. Akita M, Tak YS, Shimura T, Matsumoto S, Okuda-Shimizu Y, Shimizu Y, Nishi R, Saitoh H, Iwai S, Mori T, Ikura T, Sakai W, Hanaoka F, Sugasawa K.; ''SUMOylation of xeroderma pigmentosum group C protein regulates DNA damage recognition during nucleotide excision repair.''; PubMed Europe PMC Scholia
  63. Oksenych V, Bernardes de Jesus B, Zhovmer A, Egly JM, Coin F.; ''Molecular insights into the recruitment of TFIIH to sites of DNA damage.''; PubMed Europe PMC Scholia
  64. Ahel D, Horejsí Z, Wiechens N, Polo SE, Garcia-Wilson E, Ahel I, Flynn H, Skehel M, West SC, Jackson SP, Owen-Hughes T, Boulton SJ.; ''Poly(ADP-ribose)-dependent regulation of DNA repair by the chromatin remodeling enzyme ALC1.''; PubMed Europe PMC Scholia
  65. Coin F, Marinoni JC, Rodolfo C, Fribourg S, Pedrini AM, Egly JM.; ''Mutations in the XPD helicase gene result in XP and TTD phenotypes, preventing interaction between XPD and the p44 subunit of TFIIH.''; PubMed Europe PMC Scholia
  66. Reardon JT, Ge H, Gibbs E, Sancar A, Hurwitz J, Pan ZQ.; ''Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAK and TFIIH.''; PubMed Europe PMC Scholia
  67. Groisman R, Groisman R, Kuraoka I, Chevallier O, Gaye N, Magnaldo T, Tanaka K, Kisselev AF, Harel-Bellan A, Nakatani Y.; ''CSA-dependent degradation of CSB by the ubiquitin-proteasome pathway establishes a link between complementation factors of the Cockayne syndrome.''; PubMed Europe PMC Scholia
  68. Vermeulen W, Fousteri M.; ''Mammalian transcription-coupled excision repair.''; PubMed Europe PMC Scholia
  69. Ogi T, Limsirichaikul S, Overmeer RM, Volker M, Takenaka K, Cloney R, Nakazawa Y, Niimi A, Miki Y, Jaspers NG, Mullenders LH, Yamashita S, Fousteri MI, Lehmann AR.; ''Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells.''; PubMed Europe PMC Scholia
  70. Donahue BA, Yin S, Taylor JS, Reines D, Hanawalt PC.; ''Transcript cleavage by RNA polymerase II arrested by a cyclobutane pyrimidine dimer in the DNA template.''; PubMed Europe PMC Scholia
  71. Groisman R, Groisman R, Polanowska J, Kuraoka I, Sawada J, Saijo M, Drapkin R, Kisselev AF, Tanaka K, Nakatani Y.; ''The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage.''; PubMed Europe PMC Scholia
  72. Moser J, Volker M, Kool H, Alekseev S, Vrieling H, Yasui A, van Zeeland AA, Mullenders LH.; ''The UV-damaged DNA binding protein mediates efficient targeting of the nucleotide excision repair complex to UV-induced photo lesions.''; PubMed Europe PMC Scholia
  73. Zotter A, Luijsterburg MS, Warmerdam DO, Ibrahim S, Nigg A, van Cappellen WA, Hoeijmakers JH, van Driel R, Vermeulen W, Houtsmuller AB.; ''Recruitment of the nucleotide excision repair endonuclease XPG to sites of UV-induced dna damage depends on functional TFIIH.''; PubMed Europe PMC Scholia
  74. Nishi R, Okuda Y, Watanabe E, Mori T, Iwai S, Masutani C, Sugasawa K, Hanaoka F.; ''Centrin 2 stimulates nucleotide excision repair by interacting with xeroderma pigmentosum group C protein.''; PubMed Europe PMC Scholia
  75. Perez-Oliva AB, Lachaud C, Szyniarowski P, Muñoz I, Macartney T, Hickson I, Rouse J, Alessi DR.; ''USP45 deubiquitylase controls ERCC1-XPF endonuclease-mediated DNA damage responses.''; PubMed Europe PMC Scholia
  76. Kamitani T, Kito K, Nguyen HP, Fukuda-Kamitani T, Yeh ET.; ''Characterization of a second member of the sentrin family of ubiquitin-like proteins.''; PubMed Europe PMC Scholia
  77. Nakazawa Y, Sasaki K, Mitsutake N, Matsuse M, Shimada M, Nardo T, Takahashi Y, Ohyama K, Ito K, Mishima H, Nomura M, Kinoshita A, Ono S, Takenaka K, Masuyama R, Kudo T, Slor H, Utani A, Tateishi S, Yamashita S, Stefanini M, Lehmann AR, Yoshiura K, Ogi T.; ''Mutations in UVSSA cause UV-sensitive syndrome and impair RNA polymerase IIo processing in transcription-coupled nucleotide-excision repair.''; PubMed Europe PMC Scholia
  78. Wittschieben BØ, Iwai S, Wood RD.; ''DDB1-DDB2 (xeroderma pigmentosum group E) protein complex recognizes a cyclobutane pyrimidine dimer, mismatches, apurinic/apyrimidinic sites, and compound lesions in DNA.''; PubMed Europe PMC Scholia
  79. Wakasugi M, Kasashima H, Fukase Y, Imura M, Imai R, Yamada S, Cleaver JE, Matsunaga T.; ''Physical and functional interaction between DDB and XPA in nucleotide excision repair.''; PubMed Europe PMC Scholia
  80. He Z, Henricksen LA, Wold MS, Ingles CJ.; ''RPA involvement in the damage-recognition and incision steps of nucleotide excision repair.''; PubMed Europe PMC Scholia
  81. Volker M, Moné MJ, Karmakar P, van Hoffen A, Schul W, Vermeulen W, Hoeijmakers JH, van Driel R, van Zeeland AA, Mullenders LH.; ''Sequential assembly of the nucleotide excision repair factors in vivo.''; PubMed Europe PMC Scholia
  82. Kapetanaki MG, Guerrero-Santoro J, Bisi DC, Hsieh CL, Rapić-Otrin V, Levine AS.; ''The DDB1-CUL4ADDB2 ubiquitin ligase is deficient in xeroderma pigmentosum group E and targets histone H2A at UV-damaged DNA sites.''; PubMed Europe PMC Scholia
  83. Hofmann RM, Pickart CM.; ''Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair.''; PubMed Europe PMC Scholia
  84. Sarkar S, Kiely R, McHugh PJ.; ''The Ino80 chromatin-remodeling complex restores chromatin structure during UV DNA damage repair.''; PubMed Europe PMC Scholia
  85. Mocquet V, Lainé JP, Riedl T, Yajin Z, Lee MY, Egly JM.; ''Sequential recruitment of the repair factors during NER: the role of XPG in initiating the resynthesis step.''; PubMed Europe PMC Scholia
  86. Mathieu N, Kaczmarek N, Rüthemann P, Luch A, Naegeli H.; ''DNA quality control by a lesion sensor pocket of the xeroderma pigmentosum group D helicase subunit of TFIIH.''; PubMed Europe PMC Scholia
  87. Giglia-Mari G, Giglia-Mari G, Coin F, Ranish JA, Hoogstraten D, Theil A, Wijgers N, Jaspers NG, Raams A, Argentini M, van der Spek PJ, Botta E, Stefanini M, Egly JM, Aebersold R, Hoeijmakers JH, Vermeulen W.; ''A new, tenth subunit of TFIIH is responsible for the DNA repair syndrome trichothiodystrophy group A.''; PubMed Europe PMC Scholia
  88. Fischer ES, Scrima A, Böhm K, Matsumoto S, Lingaraju GM, Faty M, Yasuda T, Cavadini S, Wakasugi M, Hanaoka F, Iwai S, Gut H, Sugasawa K, Thomä NH.; ''The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation.''; PubMed Europe PMC Scholia
  89. Staresincic L, Fagbemi AF, Enzlin JH, Gourdin AM, Wijgers N, Dunand-Sauthier I, Giglia-Mari G, Giglia-Mari G, Clarkson SG, Vermeulen W, Schärer OD.; ''Coordination of dual incision and repair synthesis in human nucleotide excision repair.''; PubMed Europe PMC Scholia
  90. Camenisch U, Träutlein D, Clement FC, Fei J, Leitenstorfer A, Ferrando-May E, Naegeli H.; ''Two-stage dynamic DNA quality check by xeroderma pigmentosum group C protein.''; PubMed Europe PMC Scholia
  91. de Laat WL, Appeldoorn E, Sugasawa K, Weterings E, Jaspers NG, Hoeijmakers JH.; ''DNA-binding polarity of human replication protein A positions nucleases in nucleotide excision repair.''; PubMed Europe PMC Scholia
  92. Takedachi A, Saijo M, Tanaka K.; ''DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku and contributes to the recruitment of XPA.''; PubMed Europe PMC Scholia
  93. Fitch ME, Nakajima S, Yasui A, Ford JM.; ''In vivo recruitment of XPC to UV-induced cyclobutane pyrimidine dimers by the DDB2 gene product.''; PubMed Europe PMC Scholia
  94. Sugasawa K, Okuda Y, Saijo M, Nishi R, Matsuda N, Chu G, Mori T, Iwai S, Tanaka K, Tanaka K, Hanaoka F.; ''UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex.''; PubMed Europe PMC Scholia
  95. Coin F, Oksenych V, Egly JM.; ''Distinct roles for the XPB/p52 and XPD/p44 subcomplexes of TFIIH in damaged DNA opening during nucleotide excision repair.''; PubMed Europe PMC Scholia
  96. Oh KS, Imoto K, Emmert S, Tamura D, DiGiovanna JJ, Kraemer KH.; ''Nucleotide excision repair proteins rapidly accumulate but fail to persist in human XP-E (DDB2 mutant) cells.''; PubMed Europe PMC Scholia
  97. Kuraoka I, Ito S, Wada T, Hayashida M, Lee L, Saijo M, Nakatsu Y, Matsumoto M, Matsunaga T, Handa H, Qin J, Nakatani Y, Tanaka K.; ''Isolation of XAB2 complex involved in pre-mRNA splicing, transcription, and transcription-coupled repair.''; PubMed Europe PMC Scholia
  98. van Cuijk L, van Belle GJ, van Belle GJ, Turkyilmaz Y, Poulsen SL, Janssens RC, Theil AF, Sabatella M, Lans H, Mailand N, Houtsmuller AB, Vermeulen W, Marteijn JA.; ''SUMO and ubiquitin-dependent XPC exchange drives nucleotide excision repair.''; PubMed Europe PMC Scholia
  99. Su HL, Li SS.; ''Molecular features of human ubiquitin-like SUMO genes and their encoded proteins.''; PubMed Europe PMC Scholia
  100. Morris DP, Michelotti GA, Schwinn DA.; ''Evidence that phosphorylation of the RNA polymerase II carboxyl-terminal repeats is similar in yeast and humans.''; PubMed Europe PMC Scholia
  101. Lindahl T, Wood RD.; ''Quality control by DNA repair.''; PubMed Europe PMC Scholia
  102. Araújo SJ, Wood RD.; ''Protein complexes in nucleotide excision repair.''; PubMed Europe PMC Scholia

History

View all...
CompareRevisionActionTimeUserComment
115027view16:56, 25 January 2021ReactomeTeamReactome version 75
113472view11:54, 2 November 2020ReactomeTeamReactome version 74
112671view16:06, 9 October 2020ReactomeTeamReactome version 73
101588view11:45, 1 November 2018ReactomeTeamreactome version 66
101124view21:29, 31 October 2018ReactomeTeamreactome version 65
100652view20:03, 31 October 2018ReactomeTeamreactome version 64
100202view16:48, 31 October 2018ReactomeTeamreactome version 63
99753view15:14, 31 October 2018ReactomeTeamreactome version 62 (2nd attempt)
93777view13:35, 16 August 2017ReactomeTeamreactome version 61
93305view11:19, 9 August 2017ReactomeTeamreactome version 61
88077view09:04, 26 July 2016RyanmillerOntology Term : 'DNA repair pathway' added !
88076view09:02, 26 July 2016RyanmillerOntology Term : 'regulatory pathway' added !
86389view09:16, 11 July 2016ReactomeTeamreactome version 56
83072view09:53, 18 November 2015ReactomeTeamVersion54
81391view12:55, 21 August 2015ReactomeTeamVersion53
76859view08:13, 17 July 2014ReactomeTeamFixed remaining interactions
76564view11:54, 16 July 2014ReactomeTeamFixed remaining interactions
75897view09:55, 11 June 2014ReactomeTeamRe-fixing comment source
75597view10:44, 10 June 2014ReactomeTeamReactome 48 Update
74952view13:47, 8 May 2014AnweshaFixing comment source for displaying WikiPathways description
74596view08:38, 30 April 2014ReactomeTeamReactome46
42219view00:30, 8 March 2011MaintBotModified categories
42218view00:23, 8 March 2011MaintBot
42217view00:22, 8 March 2011MaintBotNew pathway

External references

DataNodes

View all...
NameTypeDatabase referenceComment
Active Pol II transcription complex with damaged DNA hybridComplexREACT_3609 (Reactome)
Active Pol II complex with repaired DNA template mRNA hybridComplexREACT_2462 (Reactome)
CCNH ProteinP51946 (Uniprot-TrEMBL)
CDK7 ProteinP50613 (Uniprot-TrEMBL)
DDB1 ProteinQ16531 (Uniprot-TrEMBL)
DDB1ProteinQ16531 (Uniprot-TrEMBL)
DDB2 ProteinQ92466 (Uniprot-TrEMBL)
DDB2ProteinQ92466 (Uniprot-TrEMBL)
DNA Polymerase delta tetramerComplexREACT_5801 (Reactome)
DNA polymerase epsilonComplexREACT_4621 (Reactome)
ERCC1 XPF complexComplexREACT_3454 (Reactome)
ERCC1 ProteinP07992 (Uniprot-TrEMBL)
ERCC1ProteinP07992 (Uniprot-TrEMBL)
ERCC2 ProteinP18074 (Uniprot-TrEMBL)
ERCC3 ProteinP19447 (Uniprot-TrEMBL)
ERCC4 ProteinQ92889 (Uniprot-TrEMBL)
ERCC4ProteinQ92889 (Uniprot-TrEMBL)
ERCC5 ProteinP28715 (Uniprot-TrEMBL)
ERCC5ProteinP28715 (Uniprot-TrEMBL)
ERCC6 ProteinQ03468 (Uniprot-TrEMBL)
ERCC6ProteinQ03468 (Uniprot-TrEMBL)
ERCC8 ProteinQ13216 (Uniprot-TrEMBL)
ERCC8ProteinQ13216 (Uniprot-TrEMBL)
GTF2H1ProteinP32780 (Uniprot-TrEMBL)
GTF2H2 ProteinQ13888 (Uniprot-TrEMBL)
GTF2H3 ProteinQ13889 (Uniprot-TrEMBL)
GTF2H4 ProteinQ92759 (Uniprot-TrEMBL)
Incision complex with 3'-incised damaged DNAComplexREACT_5099 (Reactome)
LIG1ProteinP18858 (Uniprot-TrEMBL)
MNAT1 ProteinP51948 (Uniprot-TrEMBL)
PCNA ProteinP12004 (Uniprot-TrEMBL)
PCNA homotrimerComplexREACT_2542 (Reactome)
POLD1 ProteinP28340 (Uniprot-TrEMBL)
POLD2 ProteinP49005 (Uniprot-TrEMBL)
POLD3 ProteinQ15054 (Uniprot-TrEMBL)
POLD4 ProteinQ9HCU8 (Uniprot-TrEMBL)
POLE ProteinQ07864 (Uniprot-TrEMBL)
POLE2 ProteinP56282 (Uniprot-TrEMBL)
POLR2B ProteinP30876 (Uniprot-TrEMBL)
POLR2C ProteinP19387 (Uniprot-TrEMBL)
POLR2DProteinO15514 (Uniprot-TrEMBL)
POLR2E ProteinP19388 (Uniprot-TrEMBL)
POLR2FProteinP61218 (Uniprot-TrEMBL)
POLR2G ProteinP62487 (Uniprot-TrEMBL)
POLR2H ProteinP52434 (Uniprot-TrEMBL)
POLR2I ProteinP36954 (Uniprot-TrEMBL)
POLR2J ProteinP52435 (Uniprot-TrEMBL)
POLR2K ProteinP53803 (Uniprot-TrEMBL)
POLR2L ProteinP62875 (Uniprot-TrEMBL)
RAD23B ProteinP54727 (Uniprot-TrEMBL)
RAD23BProteinP54727 (Uniprot-TrEMBL)
RFC HeteropentamerComplexREACT_4881 (Reactome)
RFC1 ProteinP35251 (Uniprot-TrEMBL)
RFC2 ProteinP35250 (Uniprot-TrEMBL)
RFC3 ProteinP40938 (Uniprot-TrEMBL)
RFC4 ProteinP35249 (Uniprot-TrEMBL)
RFC5 ProteinP40937 (Uniprot-TrEMBL)
RNA Polymerase II holoenzyme complex ComplexREACT_4889 (Reactome)
RPA heterotrimerComplexREACT_3427 (Reactome)
RPA1 ProteinP27694 (Uniprot-TrEMBL)
RPA2 ProteinP15927 (Uniprot-TrEMBL)
RPA3 ProteinP35244 (Uniprot-TrEMBL)
Repaired double-stranded DNAREACT_4318 (Reactome)
Stalled Pol II complex with damaged DNA hybridComplexREACT_3072 (Reactome)
Stalled Pol II in TC-NERComplexREACT_4443 (Reactome)
TCEA1 ProteinP23193 (Uniprot-TrEMBL)
TCEA1ProteinP23193 (Uniprot-TrEMBL)
TFIIHComplexREACT_3832 (Reactome)
Transcription-coupled ComplexREACT_3969 (Reactome)
XAB2 ProteinQ9HCS7 (Uniprot-TrEMBL)
XAB2ProteinQ9HCS7 (Uniprot-TrEMBL)
XPAProteinP23025 (Uniprot-TrEMBL)
XPC

HR23B

damaged DNA complex		ComplexREACT_3245 (Reactome)
XPC HR23B complexComplexREACT_4017 (Reactome)
XPC ProteinQ01831 (Uniprot-TrEMBL)
XPCProteinQ01831 (Uniprot-TrEMBL)
dNTPREACT_2960 (Reactome)
damaged DNA substrate nascent mRNA hybrid with 3' incisionREACT_2845 (Reactome)
damaged DNA substrate nascent mRNA hybrid with dual incisionsREACT_5428 (Reactome)
damaged DNA substrate nascent mRNA hybridREACT_3022 (Reactome)
damaged DNA substrateREACT_2765 (Reactome)
excised DNA fragment with lesionREACT_2556 (Reactome)
incised DNA without lesionREACT_3852 (Reactome)
incision complex for GG-NERComplexREACT_5159 (Reactome)
newly synthesized DNA fragmentREACT_3448 (Reactome)
p-S2,S5-POLR2A ProteinP24928 (Uniprot-TrEMBL)
pre-incision complex in GG-NERComplexREACT_5795 (Reactome)
pre-incision complex with open DNA bubbleComplexREACT_5689 (Reactome)
repaired DNA template nascent mRNA hybridREACT_5433 (Reactome)

Annotated Interactions

View all...
SourceTargetTypeDatabase referenceComment
DDB1ArrowREACT_1311 (Reactome)
DDB1REACT_1492 (Reactome)
DDB2ArrowREACT_1311 (Reactome)
DDB2REACT_1492 (Reactome)
DNA Polymerase delta tetramerArrowREACT_1196 (Reactome)
DNA Polymerase delta tetramerArrowREACT_465 (Reactome)
DNA Polymerase delta tetramerREACT_1196 (Reactome)
DNA Polymerase delta tetramerREACT_465 (Reactome)
DNA Polymerase delta tetramermim-catalysisREACT_1196 (Reactome)
DNA Polymerase delta tetramermim-catalysisREACT_465 (Reactome)
DNA polymerase epsilonArrowREACT_353 (Reactome)
DNA polymerase epsilonArrowREACT_677 (Reactome)
DNA polymerase epsilonREACT_353 (Reactome)
DNA polymerase epsilonREACT_677 (Reactome)
DNA polymerase epsilonmim-catalysisREACT_353 (Reactome)
DNA polymerase epsilonmim-catalysisREACT_677 (Reactome)
ERCC1 XPF complexArrowREACT_1311 (Reactome)
ERCC1 XPF complexREACT_1584 (Reactome)
ERCC1 XPF complexREACT_2163 (Reactome)
ERCC1REACT_1438 (Reactome)
ERCC4REACT_1438 (Reactome)
ERCC5ArrowREACT_1311 (Reactome)
ERCC5REACT_1492 (Reactome)
ERCC5REACT_1584 (Reactome)
ERCC6REACT_1584 (Reactome)
ERCC8REACT_1584 (Reactome)
Incision complex with 3'-incised damaged DNAmim-catalysisREACT_1311 (Reactome)
LIG1ArrowREACT_2181 (Reactome)
LIG1ArrowREACT_527 (Reactome)
LIG1REACT_2181 (Reactome)
LIG1REACT_527 (Reactome)
LIG1mim-catalysisREACT_2181 (Reactome)
LIG1mim-catalysisREACT_527 (Reactome)
PCNA homotrimerArrowREACT_1196 (Reactome)
PCNA homotrimerArrowREACT_353 (Reactome)
PCNA homotrimerArrowREACT_465 (Reactome)
PCNA homotrimerArrowREACT_677 (Reactome)
PCNA homotrimerREACT_1196 (Reactome)
PCNA homotrimerREACT_353 (Reactome)
PCNA homotrimerREACT_465 (Reactome)
PCNA homotrimerREACT_677 (Reactome)
RAD23BREACT_1984 (Reactome)
REACT_1033 (Reactome) Two DNA helicases XPG and XPD, which are part of TFIIH, unwind DNA duplex around this lesion to form an open bubble structure that exposes the damaged site.

REACT_1124 (Reactome) The cleavage of the damaged strand of DNA 3' to the site of damage occurs at the junction of single-stranded DNA and double-stranded DNA that is formed when the DNA duplex is unwound. The incision is carried out by XPG.

REACT_1196 (Reactome) At the beginning of this reaction, 1 molecule of 'dNTP', 1 molecule of 'RPA heterotrimer', 1 molecule of 'PCNA homotrimer', 1 molecule of 'RFC Heteropentamer', 1 molecule of 'DNA Polymerase delta tetramer', and 1 molecule of 'damaged DNA substrate:nascent mRNA hybrid with dual incisions' are present. At the end of this reaction, 1 molecule of 'RPA heterotrimer', 1 molecule of 'PCNA homotrimer', 1 molecule of 'RFC Heteropentamer', 1 molecule of 'newly synthesized DNA fragment ', 1 molecule of 'DNA Polymerase delta tetramer', and 1 molecule of 'damaged DNA substrate:nascent mRNA hybrid with dual incisions' are present.

This reaction takes place in the 'nucleus' and is mediated by the 'delta DNA polymerase activity' of 'DNA Polymerase delta tetramer'.

REACT_1274 (Reactome) At the beginning of this reaction, 1 molecule of 'Transcription-coupled (TC) repair complex', and 1 molecule of 'damaged DNA substrate:nascent mRNA hybrid' are present. At the end of this reaction, 1 molecule of 'Transcription-coupled (TC) repair complex', and 1 molecule of 'damaged DNA substrate:nascent mRNA hybrid with 3' incision' are present.

This reaction takes place in the 'nucleus' and is mediated by the 'endodeoxyribonuclease activity' of 'Transcription-coupled (TC) repair complex'.

REACT_1284 (Reactome) At the beginning of this reaction, 1 molecule of 'Transcription-coupled (TC) repair complex', and 1 molecule of 'Stalled Pol II complex with damaged DNA hybrid' are present. At the end of this reaction, 1 molecule of 'Transcription-coupled (TC) repair complex', 1 molecule of 'Stalled Pol II in TC-NER', and 1 molecule of 'damaged DNA substrate:nascent mRNA hybrid' are present.

This reaction takes place in the 'nucleus'.

REACT_1311 (Reactome) The cleavage of the damaged strand of DNA 5' to the site of damage occurs at the junction of single-stranded DNA and double-stranded DNA that is formed when the DNA duplex is unwound. The incision is carried out by ERCC1-XPF complex.

REACT_1438 (Reactome) At the beginning of this reaction, 1 molecule of 'ERCC1, DNA excision repair protein', and 1 molecule of 'XPF protein' are present. At the end of this reaction, 1 molecule of 'ERCC1:XPF complex' is present.

This reaction takes place in the 'nucleus'.

REACT_1453 (Reactome) An active Pol II complex consisting mainly of the Pol II holoenzyme transcribes the damaged DNA template.

REACT_1492 (Reactome) Transcription factor II H (TFIIH) and XPG are added to the damaged site on the DNA to form a pre-incision complex along with lesioned DNA template.

REACT_1584 (Reactome) A proper assembly of repair complex may require displacement of Pol II from the damage site exposing a significant length of the corresponding template DNA with the lesions. Speculations on the mode of this displacement of Pol II are available from experimental evidences: a. CSB mediated dissociation of Pol II b. degradation of Pol II c. CSB mediated remodeling of damaged DNA-RNA PII interface etc.
The TC-repair complex now consists of damaged DNA template: nascent mRNA hybrid. The damage site needs to be exposed to subsequent endonuclease activities.

REACT_1947 (Reactome) At the site of damage, the Pol II complex is arrested resulting in reduced levels of transcription. Several models have been proposed to explain the mechanism of this transcriptional downregulation. These include a. hyperphosphorylation of Pol II resulting in aborted entry to new pre-initiation complexes b. sequestration of TATA-binding proteins (TBP) c. enhanced use of TFIIH complexes for repair purposes precluding their use in transcription.

REACT_1984 (Reactome) XPC is mutated in individuals with xeroderma pigmentosum from genetic Complementation Group C (XP-C). It forms a tight heterodimeric complex with human Rad 23B homolog, HR23B and is thought to bind to the damaged site with lesion first triggering subsequent reactions

REACT_1987 (Reactome) Disruption of normal Watson-Crick base pairing and altered chemistry in the damaged strand involving bases may act as signals of damage that are recognized by XPC:HR23B complex.

REACT_2163 (Reactome) ERCC1-XPF complex with 5’ endonuclease activity binds to this pre-incision complex around the bubble structure to form an active incision complex.

REACT_2181 (Reactome) DNA Ligase 1 ligates the newly synthesized fragment to the gap in the template DNA.

REACT_353 (Reactome) At the beginning of this reaction, 1 molecule of 'dNTP', 1 molecule of 'incised DNA without lesion', 1 molecule of 'RPA heterotrimer', 1 molecule of 'PCNA homotrimer', 1 molecule of 'RFC Heteropentamer', and 1 molecule of 'DNA polymerase epsilon' are present. At the end of this reaction, 1 molecule of 'incised DNA without lesion', 1 molecule of 'RPA heterotrimer', 1 molecule of 'PCNA homotrimer', 1 molecule of 'RFC Heteropentamer', 1 molecule of 'newly synthesized DNA fragment ', and 1 molecule of 'DNA polymerase epsilon' are present.

This reaction takes place in the 'nucleus' and is mediated by the 'DNA-directed DNA polymerase activity' of 'DNA polymerase epsilon'.

REACT_465 (Reactome) At the beginning of this reaction, 1 molecule of 'dNTP', 1 molecule of 'incised DNA without lesion', 1 molecule of 'RPA heterotrimer', 1 molecule of 'PCNA homotrimer', 1 molecule of 'RFC Heteropentamer', and 1 molecule of 'DNA Polymerase delta tetramer' are present. At the end of this reaction, 1 molecule of 'incised DNA without lesion', 1 molecule of 'RPA heterotrimer', 1 molecule of 'PCNA homotrimer', 1 molecule of 'RFC Heteropentamer', 1 molecule of 'newly synthesized DNA fragment ', and 1 molecule of 'DNA Polymerase delta tetramer' are present.

This reaction takes place in the 'nucleus' and is mediated by the 'delta DNA polymerase activity' of 'DNA Polymerase delta tetramer'.

REACT_527 (Reactome) At the beginning of this reaction, 1 molecule of 'newly synthesized DNA fragment ', 1 molecule of 'DNA ligase I ', and 1 molecule of 'damaged DNA substrate:nascent mRNA hybrid with dual incisions' are present. At the end of this reaction, 1 molecule of 'repaired DNA template:nascent mRNA hybrid', and 1 molecule of 'DNA ligase I ' are present.

This reaction takes place in the 'nucleus' and is mediated by the 'DNA ligase activity' of 'DNA ligase I '.

REACT_551 (Reactome) At the beginning of this reaction, 1 molecule of 'Stalled Pol II in TC-NER', and 1 molecule of 'repaired DNA template:nascent mRNA hybrid' are present. At the end of this reaction, 1 molecule of 'Active Pol II complex with repaired DNA template:mRNA hybrid' is present.

This reaction takes place in the 'nucleus'.

REACT_677 (Reactome) At the beginning of this reaction, 1 molecule of 'dNTP', 1 molecule of 'RPA heterotrimer', 1 molecule of 'PCNA homotrimer', 1 molecule of 'RFC Heteropentamer', 1 molecule of 'DNA polymerase epsilon', and 1 molecule of 'damaged DNA substrate:nascent mRNA hybrid with dual incisions' are present. At the end of this reaction, 1 molecule of 'RPA heterotrimer', 1 molecule of 'PCNA homotrimer', 1 molecule of 'RFC Heteropentamer', 1 molecule of 'newly synthesized DNA fragment ', 1 molecule of 'DNA polymerase epsilon', and 1 molecule of 'damaged DNA substrate:nascent mRNA hybrid with dual incisions' are present.

This reaction takes place in the 'nucleus' and is mediated by the 'DNA-directed DNA polymerase activity' of 'DNA polymerase epsilon'.

REACT_811 (Reactome) At the beginning of this reaction, 1 molecule of 'Transcription-coupled (TC) repair complex', and 1 molecule of 'damaged DNA substrate:nascent mRNA hybrid with 3' incision' are present. At the end of this reaction, 1 molecule of 'Transcription-coupled (TC) repair complex', 1 molecule of 'excised DNA fragment with lesion', and 1 molecule of 'damaged DNA substrate:nascent mRNA hybrid with dual incisions' are present.

This reaction takes place in the 'nucleus' and is mediated by the 'endodeoxyribonuclease activity' of 'Transcription-coupled (TC) repair complex'.

RFC HeteropentamerArrowREACT_1196 (Reactome)
RFC HeteropentamerArrowREACT_353 (Reactome)
RFC HeteropentamerArrowREACT_465 (Reactome)
RFC HeteropentamerArrowREACT_677 (Reactome)
RFC HeteropentamerREACT_1196 (Reactome)
RFC HeteropentamerREACT_353 (Reactome)
RFC HeteropentamerREACT_465 (Reactome)
RFC HeteropentamerREACT_677 (Reactome)
RNA Polymerase II holoenzyme complex REACT_1453 (Reactome)
RPA heterotrimerArrowREACT_1196 (Reactome)
RPA heterotrimerArrowREACT_1311 (Reactome)
RPA heterotrimerArrowREACT_353 (Reactome)
RPA heterotrimerArrowREACT_465 (Reactome)
RPA heterotrimerArrowREACT_677 (Reactome)
RPA heterotrimerREACT_1196 (Reactome)
RPA heterotrimerREACT_1492 (Reactome)
RPA heterotrimerREACT_353 (Reactome)
RPA heterotrimerREACT_465 (Reactome)
RPA heterotrimerREACT_677 (Reactome)
Repaired double-stranded DNAArrowREACT_2181 (Reactome)
Stalled Pol II complex with damaged DNA hybridArrowREACT_1584 (Reactome)
Stalled Pol II complex with damaged DNA hybridREACT_1284 (Reactome)
Stalled Pol II complex with damaged DNA hybridREACT_1584 (Reactome)
Stalled Pol II in TC-NERArrowREACT_1284 (Reactome)
Stalled Pol II in TC-NERREACT_551 (Reactome)
TCEA1REACT_1584 (Reactome)
TFIIHArrowREACT_1311 (Reactome)
TFIIHREACT_1492 (Reactome)
TFIIHREACT_1584 (Reactome)
Transcription-coupled ArrowREACT_1274 (Reactome)
Transcription-coupled ArrowREACT_1284 (Reactome)
Transcription-coupled ArrowREACT_1584 (Reactome)
Transcription-coupled ArrowREACT_811 (Reactome)
Transcription-coupled REACT_1274 (Reactome)
Transcription-coupled REACT_1284 (Reactome)
Transcription-coupled REACT_811 (Reactome)
Transcription-coupled mim-catalysisREACT_1274 (Reactome)
Transcription-coupled mim-catalysisREACT_811 (Reactome)
XAB2REACT_1584 (Reactome)
XPAArrowREACT_1311 (Reactome)
XPAREACT_1492 (Reactome)
XPC

HR23B

damaged DNA complex		REACT_1492 (Reactome)
XPC HR23B complexArrowREACT_1311 (Reactome)
XPC HR23B complexREACT_1987 (Reactome)
XPCREACT_1984 (Reactome)
dNTPREACT_1196 (Reactome)
dNTPREACT_353 (Reactome)
dNTPREACT_465 (Reactome)
dNTPREACT_677 (Reactome)
damaged DNA substrate nascent mRNA hybrid with 3' incisionArrowREACT_1274 (Reactome)
damaged DNA substrate nascent mRNA hybrid with 3' incisionREACT_811 (Reactome)
damaged DNA substrate nascent mRNA hybrid with dual incisionsArrowREACT_1196 (Reactome)
damaged DNA substrate nascent mRNA hybrid with dual incisionsArrowREACT_677 (Reactome)
damaged DNA substrate nascent mRNA hybrid with dual incisionsArrowREACT_811 (Reactome)
damaged DNA substrate nascent mRNA hybrid with dual incisionsREACT_1196 (Reactome)
damaged DNA substrate nascent mRNA hybrid with dual incisionsREACT_527 (Reactome)
damaged DNA substrate nascent mRNA hybrid with dual incisionsREACT_677 (Reactome)
damaged DNA substrate nascent mRNA hybridArrowREACT_1284 (Reactome)
damaged DNA substrate nascent mRNA hybridREACT_1274 (Reactome)
damaged DNA substrate nascent mRNA hybridREACT_1453 (Reactome)
damaged DNA substrateREACT_1987 (Reactome)
excised DNA fragment with lesionArrowREACT_1311 (Reactome)
excised DNA fragment with lesionArrowREACT_811 (Reactome)
incised DNA without lesionArrowREACT_1311 (Reactome)
incised DNA without lesionArrowREACT_353 (Reactome)
incised DNA without lesionArrowREACT_465 (Reactome)
incised DNA without lesionREACT_2181 (Reactome)
incised DNA without lesionREACT_353 (Reactome)
incised DNA without lesionREACT_465 (Reactome)
incision complex for GG-NERmim-catalysisREACT_1124 (Reactome)
newly synthesized DNA fragmentArrowREACT_1196 (Reactome)
newly synthesized DNA fragmentArrowREACT_353 (Reactome)
newly synthesized DNA fragmentArrowREACT_465 (Reactome)
newly synthesized DNA fragmentArrowREACT_677 (Reactome)
newly synthesized DNA fragmentREACT_2181 (Reactome)
newly synthesized DNA fragmentREACT_527 (Reactome)
pre-incision complex in GG-NERmim-catalysisREACT_1033 (Reactome)
pre-incision complex with open DNA bubbleREACT_2163 (Reactome)
repaired DNA template nascent mRNA hybridArrowREACT_527 (Reactome)
repaired DNA template nascent mRNA hybridREACT_551 (Reactome)
Retrieved from "https://classic.wikipathways.org/index.php/Pathway:WP1980"
Personal tools