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Start a new discussionD-threo-isocitrate (1)
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What is this molecule? Clicking on it didn't lead anywhere. I know that oxalosuccinate is an unstable intermediate at around this portion of the citric acid cycle, but am unfamiliar with threo-isocitrate.CharlesHBennett 13:09, 4 November 2014 (UTC)
Hi Charles, The node labeled D-threo-isocitrate is annotated with HMDB00193, you can see the HMDB entry below. The molecule is isocitrate or D-isocitrate, which is produced by the oxidation of citrate by aconitase. The name D-threo-isocitrate is one of many synonyms for this molecule. The original author of the pathway selected this name for the node rather than the standard name, but you are welcome to change it if you believe the standard name or another of the synonyms is preferred for this molecule. http://www.hmdb.ca/metabolites/HMDB00193
Regarding oxalosuccinate, I believe this is an unstable intermediate after isocitrate (http://en.wikipedia.org/wiki/Oxalosuccinic_acid, see diagram at the bottom of the page), in the oxidative decarboxylation of isocitrate to Alpha-Ketoglutarate. Please feel free to add this step to the pathway.
Regards,
Kristina
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That would be great if you could to that. There will be a supporting article explaining why we made these changes. We will submit this article this week. I do have some questions. The text under 'This pathway contains genes, proteins or metabolites with missing database annotations' is that automatically generated? I think I have most annotation except for the references to other pathways. Another question I have is that I now use "geneProduct", I am not sure if I should have used "protein"? Furthermore, the connection between reaction and geneproduct is "catalysis", but still the system seems to prefer gene IDs instead of protein IDs. And I cannot find a way to say which gene encodes for the protein. Last question, we looked up in ChEBI for most metabolites the version of the metabolite with the correct charge for the mitochondrion. We also have the HMDB IDs which I now used, but the automatic retrieval system then gives back the "neutral" versions of the metabolites in ChEBI. I do not seem to be able to annotate a metabolite with 2 IDs.--Mdstobbe 07:46, 3 April 2012 (UTC)
1. Yes it's automatically generated. You can delete the curation tag at any time, if you feel you've addressed as many of the issues as possible. Note that our bots apply new tags after every edit, so if you're planning more edits, wait until you're done to delete the tag. 2. Up to you whether you use "geneProduct" or "protein". Both are accurate. 3. "catalysis" is appropriate. The system does not have a preference, per se, it has an alias database with gene and protein IDs. You can choose whichever you find most effective. Downstream analysis tools should offer ways to convert/unify if necessary. 4. You can not annotate a datanode with 2 IDs. You can add the terms you've looked up as a comment on the node, but only 1 ID is accepted for mapping. And I recommend using the one in the database so we can support linkouts and ID mapping, etc.
Thank you for the answers. I tried now to annotate the geneproducts with the appropriate UniProt ID, but then in the "live" version it says that "Unable to load external references.", despite that this does work when I am the "editing mode". If I use the Entrez Gene IDs to annotate a protein then the external references can be loaded. A similar thing happens for metabolites, where HMDB IDs seem to work best. The curation tag "This pathway contains genes, proteins or metabolites with missing database annotations'" now only "complains" about the references to the other pathways andnd about the metabolite H+ and ubiquinol-10. I do not know why and how to correct this.
Use the ID you think is best. The "unable to load external refs" sounds like a bug on our end. If you found it in the database and it produces external references in edit mode, then you're all set. As for the curation tag, the "correction" is to simply remove it. Just click the little red 'x' after you're done making your edits. It won't appear again until it's triggered by future edits.
The pathway is final for now and the paper submitted. I picked the Entrez Gene IDs as we needed to submit the paper and the UniProt IDs did not generate links to other databases in the live version, only in the edit mode. I would prefer UniProt IDs though as it are enzymes and not genes, so if there is a fix let me know.
Great. In your paper, be aware of the difference between citing the pathway (e.g., http://wikipathways.org/index.php/Pathway:WP78) and citing your particular revision (e.g., http://wikipathways.org/index.php?title=Pathway:WP78&oldid=47741). The former may (and will) change over time... it's a wiki after all, and knowledge evolves; while the latter will always refer to that particular version no matter what changes follow. You may want to cite both depending on the purpose of the citation. AlexanderPico 21:03, 17 April 2012 (UTC)
