Help:Tutorial:Step 8

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Draw cellular locations

Drawing a nucleus

In this step, we will add information about cellular localization to the pathway, to show which event happen in the nucleus. The Clock and Bmal1 dimerization, E-box activation, transcription of target genes and inhibition of Clock/Bmal1 by mPer/mCry all happen in the nucleus. We can show this by drawing a rectangle that defines the boundaries of the nucleus and place the above events within that boundary. After this step, the pathway will look like this:

circadian_clock_complete.png

Draw a rectangle to represent the nucleus

To indicate the boundaries of the nucleus, draw a rectangle that is big enough to contain the events that take place in the nucleus.

Add a label

To show that the rectangle represents the nucleus, add a label and set the text to 'Nucleus'. You can increase the font size to make the label stand out more, by changing the Font Size property to 200 and enabling the Bold property in the properties table.

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