mRNA Processing (Canis familiaris)

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Formation of the Spliceosomal C complexCFIAG65U2YYYYPInternal methylation of mRNA3Serine/Argenine Rich Proteins(SR)AGSR protein kinases35CF IISR-proteinsAGU4Accessory Proteinssplicing factor SF3AAGUGiPo5Primary RNA transcriptPoly(A) polymerase alphasnRNP Sm core complexKinases Phosphorylating RS domains?exon 135Poly(A)-binding protein IIexon 1Additional FactorsU2 snRNPU5P-hnRNPsSR-repressor proteinsU5Cleavage at the 3'-Splice-Site and Exon LigationAFormation of the Spliceosomal B complexCA5snRNP Sm core complexCleavage of mRNA at the 3'-endintronU6 snRNPU1Formation of pre-mRNPsCF II5 G capexon 13Additional SR proteinsCap Binding(3)CstF3SR-phosphatases5CPSFFormation of pre-mRNPs(2b)GU3Lariat Formation and 5'-Splice Site CleavagePoly(A) polYYYYMethylation of mRNAAAUAAA(2c)snRNP Sm core complexNote: New participating members shown at each step53Formation of the Spliceosomal E complexRNA helicase A5exon 2Aexon 2mRNA splicing (major pathway)U4/U6ASpt5Rngtt (capping enzyme)SR-proteinsFormation of the Spliceosomal E complexMisc.U2AFPolr2aSR-kinases5A3U2AFCPSFsplicing factor SF3BInternal methylation of mRNAUGexon 2mRNA polyadenylation(2)Formation of the Spliceosomal A complexexon 2RNA helicasemRNA cappingAAAAAAA(1)U1 snRNPmRNA splice site selectionU2 snRNPFormation of the Spliceosomal B complexFormation of the Spliceosomal A complex5Processing of Intron-Containing Pre-mRNA3U4 snRNP(2a)SR-proteinssplicesome associated helicaseshnRNP regulatorsAdditional FactorsSR-binding sitesYYYYexon 2AGPutative Alternative SplicingRegulatorsSR protein phosphatasesYYYYAdditional Factors5exon 1YYYYFormation of the active Spliceosomal C complexmRNA CappingU4:U5:U6 tri-snRNP complexAU2 snRNPsAGRegulation ofAlternative SplicingA5YYYYPoly(A)pb IIright click on blue text for detailed informationpre-mRNAexon 13SR-binding sites3U5 snRNPCstFU1 snRNP5exon 2SF3b14bexon 1PiCF Iheterogeneous nuclear ribonucleoproteins3degradedRNAi pathwayU6mRNA 3'-end processingRnmt (methyltransferase)SMC1ARBM5SNRPGYBX1RBMXNUDT21PTBP2POLR2ASFRS1SNRPB2RNGTTU4 snRNASFRS4RBM17CPSF3SNRPACLK2CSTF3LSM7SNRPFRBM39DICER1LSM2HNRNPH2HNRNPKU2AF2DNAJC8HNRPA1HNRPABCPSF2SNRPNHNRNPUPRPF4BPPM1GSFRS8CPSF1PAPOLAPRPF6HNRNPA2B1CSTF1PRPF8SRRM1DDX20SF3B2PRMT2CSTF2TDHX16CLK1SNRPESFRS16CDC40CUGBP1SSFA1PHF5ASF3B4CD2BP2U1 snRNASRP54NCBP1SPOPPRPF8SFRS12U5 snRNACSTF2SF3B3TRA2BRNMTSNRPD2DDX1SNRPA1DHX15SFPQEFTUD2CDC40HNRNPDSNRNP70PRPF3BRUNOL4SNRPBCLK4PRPF18LSM2PRPF4TXNL4AHNRNPRGMPHNRNPH1SNRPD1SNRNP40SF3A2SFRS3FUSIP1SSFA1PABPN1HNRNPMCLK3XRN2FUSRHOXF2NCBP2SF3B5SF3B1SNRPNSUPT5HRNPS1HNRPA3P1SFRS6TMED10SRPK1SFRS2SRPK2NONONXF1SF3A3SFRS9SNRPD3DHX38RNU2PRPF40AMETTL3ATPCLP1SFRS14U2AF1PTBP1HNRNPCCPSF4PRPF4HNRNPLCUGBP2DHX8SF3A1PSKH1SSFA1DHX9PRMT1FUSIP1SF4SFRS7SFRS5PCBP2


Description

No description

Comments

GenMAPP notes 
Gene lists from obtained from InterPro 9-23-2003: Mm RNA-binding region RNP-1 (RNA recognition motif)
GenMAPP remarks 
Adapted from http://www.reactome.org.
HomologyConvert 
This pathway was inferred from Homo sapiens pathway WP411(r30272) with a 80% conversion rate.

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Ontology Terms

 

Bibliography

  1. Carmody SR, Wente SR; ''mRNA nuclear export at a glance.''; J Cell Sci, 2009 PubMed Europe PMC Scholia

History

View all...
CompareRevisionActionTimeUserComment
122786view00:13, 18 May 2022AlexanderPicocleaning up broken comments
95765view14:20, 19 January 2018MkutmonHomology conversion update
71483view19:18, 17 October 2013MaintBotAutomated update of data sources
69956view20:51, 11 July 2013MaintBotupdated to 2013 schema
68581view23:08, 5 July 2013MaintBotUpdated to 2013 gpml schema
67493view11:09, 26 June 2013DdiglesOntology Term : 'spliceosome pathway' added !
48160view18:58, 9 May 2012MaintBotUpdating from human to fix xrefs
40740view21:53, 1 March 2011MaintBotRemoved redundant pathway information and comments
35243view19:50, 11 February 2010KhanspersModified description
34015view18:43, 9 December 2009MaintBotAutomatic update of empty xrefs
32569view13:12, 17 August 2009MaintBotFixed text labels
32247view12:54, 15 August 2009MaintBotFixed text labels
31762view12:06, 14 August 2009MaintBotFixed group labels
31267view19:57, 13 August 2009MaintBotFixed text labels
30711view22:53, 29 July 2009MaintBotNew pathway

External references

DataNodes

View all...
NameTypeDatabase referenceComment
ATPMetabolite
BRUNOL4GeneProduct610838 (Entrez Gene)
CD2BP2GeneProduct489937 (Entrez Gene)
CDC40GeneProduct475025 (Entrez Gene)
CLK1GeneProductPMID: 15010457: Inhibitor John C. Bell, PhD

Professor, Depts. of Medicine and Biochemistry, Microbiology & Immunology University of Ottawa Career Scientist, Ottawa Regional Cancer Centre Associate Scientist, The Ottawa Hospital Research Institute Telephone: 613-737-7700 ext 6893 Fax: 613-247-3524 Email: [email protected] The control of pre-mRNA splicing by the Clk kinase family

We are studying a family of kinases which we believe provide an interface between intracellular signaling networks and the post-transcriptional mechanism of mRNA splicing. We are performing a structure:function analysis of the three Clk family members to identify domains in the three proteins which are involved in regulating splicing. Using homologous recombination we are generating null strains of mice which are lacking one, two or all three Clk genes. The Clk kinases all possess dual specificity kinase activity and yeast expression systems are being used to produce large amounts of the kinase to perform a detailed analysis of the sites of serine, threonine and tyrosine autophosphorylation within the kinase.
CLK2GeneProduct490427 (Entrez Gene)
CLK3GeneProduct478367 (Entrez Gene)
CLK4GeneProduct474645 (Entrez Gene)
CLP1GeneProduct610161 (Entrez Gene)
CPSF1GeneProduct475126 (Entrez Gene)
CPSF2GeneProduct480230 (Entrez Gene)
CPSF3GeneProduct482962 (Entrez Gene)
CPSF4GeneProduct489859 (Entrez Gene)
CSTF1GeneProduct612933 (Entrez Gene)
CSTF2GeneProduct492015 (Entrez Gene)
CSTF2TGeneProduct
CSTF3GeneProduct475948 (Entrez Gene)
CUGBP1GeneProduct475979 (Entrez Gene)
CUGBP2GeneProduct607310 (Entrez Gene)
DDX1GeneProduct475671 (Entrez Gene)
DDX20GeneProduct475859 (Entrez Gene)
DHX15GeneProduct488856 (Entrez Gene)
DHX16GeneProduct481706 (Entrez Gene)
DHX38GeneProduct479669 (Entrez Gene)
DHX8GeneProduct480507 (Entrez Gene)
DHX9GeneProduct480032 (Entrez Gene)
DICER1GeneProduct480426 (Entrez Gene)
DNAJC8GeneProduct478166 (Entrez Gene)
EFTUD2GeneProduct490935 (Entrez Gene)
FUSGeneProduct
FUSIP1GeneProductFUSI MOUSE PMID: 11684676
GMPMetabolite
HNRNPA2B1GeneProduct475260 (Entrez Gene)
HNRNPCGeneProduct475399 (Entrez Gene)
HNRNPDGeneProduct478450 (Entrez Gene)
HNRNPH1GeneProduct481455 (Entrez Gene)
HNRNPH2GeneProduct480989 (Entrez Gene)
HNRNPKGeneProduct480979 (Entrez Gene)
HNRNPLGeneProduct476466 (Entrez Gene)
HNRNPMGeneProduct476725 (Entrez Gene)
HNRNPRGeneProduct478192 (Entrez Gene)
HNRNPUGeneProduct480100 (Entrez Gene)
HNRPA1GeneProduct
HNRPA3P1GeneProduct
HNRPABGeneProduct
LSM2GeneProduct474849 (Entrez Gene)
LSM7GeneProduct612170 (Entrez Gene)
METTL3GeneProduct475404 (Entrez Gene)
NCBP1GeneProduct474772 (Entrez Gene)
NCBP2GeneProduct478603 (Entrez Gene)
NONOGeneProduct612773 (Entrez Gene)
NUDT21GeneProduct478123 (Entrez Gene)
NXF1GeneProduct483780 (Entrez Gene)
PABPN1GeneProduct490611 (Entrez Gene)
PAPOLAGeneProduct480430 (Entrez Gene)
PCBP2GeneProduct477596 (Entrez Gene)
PHF5AGeneProduct474489 (Entrez Gene)
POLR2AGeneProduct479483 (Entrez Gene)
PPM1GGeneProduct475703 (Entrez Gene) PMID: 12773558
PRMT1GeneProduct476411 (Entrez Gene)
PRMT2GeneProduct480811 (Entrez Gene)
PRPF18GeneProduct477998 (Entrez Gene)
PRPF3GeneProduct475836 (Entrez Gene)
PRPF40AGeneProduct476152 (Entrez Gene)
PRPF4GeneProduct474807 (Entrez Gene)
PRPF4BGeneProduct488199 (Entrez Gene)
PRPF6GeneProduct477289 (Entrez Gene)
PRPF8GeneProduct480651 (Entrez Gene)
PSKH1GeneProductPMID: 12466556
PTBP1GeneProduct485097 (Entrez Gene)
PTBP2GeneProduct490149 (Entrez Gene)
RBM17GeneProduct478014 (Entrez Gene)
RBM39GeneProduct477216 (Entrez Gene)
RBM5GeneProduct476618 (Entrez Gene)
RBMXGeneProduct481063 (Entrez Gene)
RHOXF2GeneProduct611154 (Entrez Gene) GenBank sequence: M10329
RNGTTGeneProduct481914 (Entrez Gene)
RNMTGeneProduct476200 (Entrez Gene)
RNPS1GeneProduct479880 (Entrez Gene) PMID: 14729963
RNU2GeneProductGenBank sequence: K00027
SF3A1GeneProduct477538 (Entrez Gene)
SF3A2GeneProduct612188 (Entrez Gene)
SF3A3GeneProduct
SF3B1GeneProduct488456 (Entrez Gene)
SF3B2GeneProduct476015 (Entrez Gene)
SF3B3GeneProduct479659 (Entrez Gene)
SF3B4GeneProduct483177 (Entrez Gene)
SF3B5GeneProduct611736 (Entrez Gene)
SF4GeneProduct476661 (Entrez Gene) inferred from: SURP 2, G patch protein, UniProt:O60378
SFPQGeneProduct
SFRS12GeneProduct487233 (Entrez Gene) PMID: 14559993. SRrp86 is a unique member of the SR protein superfamily containing one RNA recognition motif and two serine-arginine (SR)-rich domains separated by an unusual glutamic acid-lysine (EK)-rich region. Previously, we showed that SRrp86 could regulate alternative splicing by both positively and negatively modulating the activity of other SR proteins and that the unique EK domain could inhibit both constitutive and alternative splicing. These functions were most consistent with the model in which SRrp86 functions by interacting with and thereby modulating the activity of target proteins. To identify the specific proteins that interact with SRrp86, we used a yeast two-hybrid library screen and immunoprecipitation coupled to mass spectrometry. We show that SRrp86 interacts with all of the core SR proteins, as well as a subset of other splicing regulatory proteins, including SAF-B, hnRNP G, YB-1, and p72. In contrast to previous results that showed activation of SRp20 by SRrp86, we now show that SAF-B, hnRNP G, and 9G8 all antagonize the activity of SRrp86. Overall, we conclude that not only does SRrp86 regulate SR protein activity but that it is, in turn, regulated by other splicing factors to control alternative splice site selection.
SFRS14GeneProduct487882 (Entrez Gene)
SFRS1GeneProduct609693 (Entrez Gene) Antagonist splicing factor
SFRS16GeneProduct476436 (Entrez Gene)
SFRS2GeneProduct612817 (Entrez Gene)
SFRS3GeneProduct403687 (Entrez Gene)
SFRS4GeneProduct
SFRS5GeneProduct490754 (Entrez Gene)
SFRS6GeneProduct
SFRS7GeneProduct475730 (Entrez Gene)
SFRS8GeneProduct477438 (Entrez Gene)
SFRS9GeneProduct477510 (Entrez Gene) PMID: 12738786, PMID: 12024014 (associated with hnRNP A1 splicing)
SMC1AGeneProduct480928 (Entrez Gene)
SNRNP40GeneProduct611914 (Entrez Gene)
SNRNP70GeneProduct484388 (Entrez Gene)
SNRPA1GeneProduct607102 (Entrez Gene)
SNRPAGeneProduct476454 (Entrez Gene)
SNRPB2GeneProduct479267 (Entrez Gene)
SNRPBGeneProduct485806 (Entrez Gene)
SNRPD1GeneProduct480178 (Entrez Gene)
SNRPD2GeneProduct
SNRPD3GeneProduct607772 (Entrez Gene)
SNRPEGeneProduct478934 (Entrez Gene)
SNRPFGeneProduct482609 (Entrez Gene)
SNRPGGeneProduct
SNRPNGeneProduct479012 (Entrez Gene)
SPOPGeneProduct480548 (Entrez Gene)
SRP54GeneProduct403953 (Entrez Gene) PMID: 14729963
SRPK1GeneProduct474887 (Entrez Gene) PMID: 12773558
SRPK2GeneProduct475894 (Entrez Gene) PMID: 12773558
SRRM1GeneProduct478185 (Entrez Gene)
SSFA1GeneProduct
SUPT5HGeneProduct476462 (Entrez Gene)
TMED10GeneProduct610559 (Entrez Gene)
TRA2BGeneProduct478663 (Entrez Gene)
TXNL4AGeneProduct476156 (Entrez Gene)
U1 snRNAGeneProductGenBank sequence: J00645
U2AF1GeneProduct478422 (Entrez Gene)
U2AF2GeneProduct
U4 snRNAGeneProductGenBank sequence: M10328, M18004
U5 snRNAGeneProductGenBank sequence: AB021173
XRN2GeneProduct477133 (Entrez Gene)
YBX1GeneProduct479011 (Entrez Gene)

Annotated Interactions

No annotated interactions
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