Processing of Capped Intron-Containing Pre-mRNA (Homo sapiens)

From WikiPathways

Revision as of 21:57, 4 March 2011 by MaintBot (Talk | contribs)
Jump to: navigation, search
57, 86, 77, 811-136, 76, 714, 1515, 169, 10cytosolnucleoplasmSpliceosomal A ComplexMature intronless transcript derived Histone mRNA:SLBP:TAP:Aly/Ref complexATAC C ComplexSMC2 (P-loop, DA-box)mRNA 3'-end cleavage factorhnRNP A0SURP 2, G patch proteinMature Intronless Transcript Derived mRNA:eIF4E ComplexCBP80Mature Intronless Transcript Derived Histone mRNA:TAP:Aly/Ref complexhPrp17THOC4(Aly/Ref)Mature intronless derived mRNA:TAP:Aly/Ref complexASR2BCF IIHuR /3 RRM proteinMature mRNP ComplexMature Intronless Transcript Derived Histone mRNA:TAP:Aly/Ref ComplexU12 snRNPhPrp18U2 snRNPCPSFNFX.1eIF4EhnRNP-E2DnaJ hom. NP_055602CF IIhUpf3BCF ITHOC4(Aly/Ref)U2AF 35 kDa subunithPrp5hnRNP H'THOC4(Aly/Ref)eIF4E3'-polyadenylated, capped mRNA complexTAPSRp20RNA polymerase II (phosphorylated):TFIIF complexMature intronless transcript derived Histone mRNA:SLBP:eIF4E ComplexhnRNP-E1hnRNP GCPSFhnRNP A2hnRNP RSR7/ 9G8 proteinSF2/ASF/SFRS1eIF4ESRm160CC1.3 protein /3 RRM, RSU4 ATAC:U5:U6 ATAC ComplexPoly(A) polymerase alphaCPSFTAPPoly(A) polymerase alphaMagoh-Y14 complexATAC A ComplexTAPPABPN1post exon ligation complexhPrp16hnRNP L3' end cleaved, ligated exon containing complexSpliceosomal Intermediate C ComplexCstFU6 ATAC snRNPhnRNP MU5 snRNPExport Receptor bound mature mRNA ComplexSRp40Export Receptor bound mature mRNA ComplexhnRNP HTHOC4(Aly/Ref)intron-containing complexSR4 / SRp75UBA, Tudor - FLJ21007hnRNP C1/C2SMC1 (BAA11495)hnRNP INuclear Pore Complex (NPC)TAPE1B-AP5 nucleocytoplasmic transport proteinSRm160CF ICap Binding Complex (CBC)NFAR-2 proteincapped, methylated pre-mRNP:CBC complexSRp55U4:U5:U6 trisnRNP complexNucleoplasmic mature intronless derived mRNA:TAP:Aly/Ref complexmRNA (N6-adenosine)-methyltransferaseU11 snRNPCleavage and Polyadenylation ComplexU1 snRNPSpliceosomal active C complex with lariat containing, 5'-end cleaved pre-mRNP:CBC complexMature intronless transcript derived Histone pre-mRNA:CBC complexRNPS1Spliceosomal B ComplexATAC C Complex with lariat containing 5'-end cleaved mRNAhTra2U4 ATAC snRNPhnRNP UFUS - RNA binding protein/TLShPrp43hnRNP FExon Junction ComplexDDX9/NDH IIhnRNP A1hnRNP KYB-1FLJ10839 DNA-binding proteinTAP:3'-polyadenylated, capped mRNA complexRBM 5capped, methylated pre-mRNA:CBC ComplexU2AF 65 kDa subunitATAC B Complexcapped pre-mRNA:CBC:RNA Pol II (phosphorylated) complexhSLU7p68 DEAH proteinMature Intronless transcript derived Histone mRNA:SLBP:CBP80:CBP20TAPPABPN1CstFU1 snRNPTAPNuclear Pore Complex (NPC)SR9 / SRp30Mature SLBP independent Histone mRNA:eIF4E complexhnRNP A3Mature intronless transcript derived Histone mRNA:SLBP:TAP:Aly/Ref complexU4 snRNPhPrp19SPF31 (Dna J)hnRNP D0SR 11/ p54hPrp22Mature intronless derived mRNA complexSpliceosomal E ComplexSpliceosomal Active C ComplexLigated exon containing complexCD2BP2 - GYFATPCBP20SR2 / SC352-412-4


Description

Co-transcriptional pre-mRNA splicing is not obligatory. Pre-mRNA splicing begins co-transcriptionally and often continues post-transcriptionally. Human genes contain an average of nine introns per gene, which cannot serve as splicing substrates until both 5' and 3' ends of each intron are synthesized. Thus the time that it takes for pol II to synthesize each intron defines a minimal time and distance along the gene in which splicing factors can be recruited. The time that it takes for pol II to reach the end of the gene defines the maximal time in which splicing could occur co-transcriptionally. Thus, the kinetics of transcription can affect the kinetics of splicing.Any covalent change in a primary (nascent) mRNA transcript is mRNA Processing. For successful gene expression, the primary mRNA transcript needs to be converted to a mature mRNA prior to its translation into polypeptide. Eucaryotic mRNAs undergo a series of complex processing reactions; these begin on nascent transcripts as soon as a few ribonucleotides have been synthesized during transcription by RNA Polymerase II, through the export of the mature mRNA to the cytoplasm, and culminate with mRNA turnover in the cytoplasm.

Try the New WikiPathways

View approved pathways at the new wikipathways.org.

Quality Tags

Ontology Terms

 

Bibliography

View all...
  1. Hutten S, Kehlenbach RH.; ''CRM1-mediated nuclear export: to the pore and beyond.''; PubMed Europe PMC Scholia
  2. Lévesque L, Guzik B, Guan T, Coyle J, Black BE, Rekosh D, Hammarskjöld ML, Paschal BM.; ''RNA export mediated by tap involves NXT1-dependent interactions with the nuclear pore complex.''; PubMed Europe PMC Scholia
  3. Coltri P, Effenberger K, Chalkley RJ, Burlingame AL, Jurica MS.; ''Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate.''; PubMed Europe PMC Scholia
  4. Zhou Z, Licklider LJ, Gygi SP, Reed R.; ''Comprehensive proteomic analysis of the human spliceosome.''; PubMed Europe PMC Scholia
  5. Le Hir H, Izaurralde E, Maquat LE, Moore MJ.; ''The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.''; PubMed Europe PMC Scholia
  6. Ma XM, Yoon SO, Richardson CJ, Jülich K, Blenis J.; ''SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs.''; PubMed Europe PMC Scholia
  7. Ajuh P, Sleeman J, Chusainow J, Lamond AI.; ''A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing.''; PubMed Europe PMC Scholia
  8. Hegele A, Kamburov A, Grossmann A, Sourlis C, Wowro S, Weimann M, Will CL, Pena V, Lührmann R, Stelzl U.; ''Dynamic protein-protein interaction wiring of the human spliceosome.''; PubMed Europe PMC Scholia
  9. Pikielny CW, Bindereif A, Green MR.; ''In vitro reconstitution of snRNPs: a reconstituted U4/U6 snRNP participates in splicing complex formation.''; PubMed Europe PMC Scholia
  10. Kataoka N, Dreyfuss G.; ''A simple whole cell lysate system for in vitro splicing reveals a stepwise assembly of the exon-exon junction complex.''; PubMed Europe PMC Scholia
  11. Hung ML, Hautbergue GM, Snijders AP, Dickman MJ, Wilson SA.; ''Arginine methylation of REF/ALY promotes efficient handover of mRNA to TAP/NXF1.''; PubMed Europe PMC Scholia
  12. Cronshaw JM, Krutchinsky AN, Zhang W, Chait BT, Matunis MJ.; ''Proteomic analysis of the mammalian nuclear pore complex.''; PubMed Europe PMC Scholia
  13. Suyama M, Doerks T, Braun IC, Sattler M, Izaurralde E, Bork P.; ''Prediction of structural domains of TAP reveals details of its interaction with p15 and nucleoporins.''; PubMed Europe PMC Scholia
  14. Grote M, Wolf E, Will CL, Lemm I, Agafonov DE, Schomburg A, Fischle W, Urlaub H, Lührmann R.; ''Molecular architecture of the human Prp19/CDC5L complex.''; PubMed Europe PMC Scholia
  15. Moore CL, Sharp PA.; ''Accurate cleavage and polyadenylation of exogenous RNA substrate.''; PubMed Europe PMC Scholia
  16. Blencowe BJ, Baurén G, Eldridge AG, Issner R, Nickerson JA, Rosonina E, Sharp PA.; ''The SRm160/300 splicing coactivator subunits.''; PubMed Europe PMC Scholia
  17. Le Hir H, Gatfield D, Izaurralde E, Moore MJ.; ''The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.''; PubMed Europe PMC Scholia
  18. Liu J, Yue Y, Han D, Wang X, Fu Y, Zhang L, Jia G, Yu M, Lu Z, Deng X, Dai Q, Chen W, He C.; ''A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.''; PubMed Europe PMC Scholia
  19. Bokar JA, Shambaugh ME, Polayes D, Matera AG, Rottman FM.; ''Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase.''; PubMed Europe PMC Scholia
  20. Lindtner S, Felber BK, Kjems J.; ''An element in the 3' untranslated region of human LINE-1 retrotransposon mRNA binds NXF1(TAP) and can function as a nuclear export element.''; PubMed Europe PMC Scholia
  21. Katahira J, Straesser K, Saiwaki T, Yoneda Y, Hurt E.; ''Complex formation between Tap and p15 affects binding to FG-repeat nucleoporins and nucleocytoplasmic shuttling.''; PubMed Europe PMC Scholia
  22. Chi B, Wang Q, Wu G, Tan M, Wang L, Shi M, Chang X, Cheng H.; ''Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA.''; PubMed Europe PMC Scholia
  23. Ilagan JO, Chalkley RJ, Burlingame AL, Jurica MS.; ''Rearrangements within human spliceosomes captured after exon ligation.''; PubMed Europe PMC Scholia
  24. Makarov EM, Owen N, Bottrill A, Makarova OV.; ''Functional mammalian spliceosomal complex E contains SMN complex proteins in addition to U1 and U2 snRNPs.''; PubMed Europe PMC Scholia
  25. Suntharalingam M, Wente SR.; ''Peering through the pore: nuclear pore complex structure, assembly, and function.''; PubMed Europe PMC Scholia
  26. Ping XL, Sun BF, Wang L, Xiao W, Yang X, Wang WJ, Adhikari S, Shi Y, Lv Y, Chen YS, Zhao X, Li A, Yang Y, Dahal U, Lou XM, Liu X, Huang J, Yuan WP, Zhu XF, Cheng T, Zhao YL, Wang X, Rendtlew Danielsen JM, Liu F, Yang YG.; ''Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase.''; PubMed Europe PMC Scholia
  27. Zhou Z, Luo MJ, Straesser K, Katahira J, Hurt E, Reed R.; ''The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans.''; PubMed Europe PMC Scholia
  28. Cheng H, Dufu K, Lee CS, Hsu JL, Dias A, Reed R.; ''Human mRNA export machinery recruited to the 5' end of mRNA.''; PubMed Europe PMC Scholia
  29. Yamazaki T, Fujiwara N, Yukinaga H, Ebisuya M, Shiki T, Kurihara T, Kioka N, Kambe T, Nagao M, Nishida E, Masuda S.; ''The closely related RNA helicases, UAP56 and URH49, preferentially form distinct mRNA export machineries and coordinately regulate mitotic progression.''; PubMed Europe PMC Scholia
  30. Makarov EM, Makarova OV, Urlaub H, Gentzel M, Will CL, Wilm M, Lührmann R.; ''Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome.''; PubMed Europe PMC Scholia
  31. Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R.; ''Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.''; PubMed Europe PMC Scholia
  32. Schwartz S, Mumbach MR, Jovanovic M, Wang T, Maciag K, Bushkin GG, Mertins P, Ter-Ovanesyan D, Habib N, Cacchiarelli D, Sanjana NE, Freinkman E, Pacold ME, Satija R, Mikkelsen TS, Hacohen N, Zhang F, Carr SA, Lander ES, Regev A.; ''Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5' sites.''; PubMed Europe PMC Scholia
  33. Wu Q, Krainer AR.; ''AT-AC pre-mRNA splicing mechanisms and conservation of minor introns in voltage-gated ion channel genes.''; PubMed Europe PMC Scholia
  34. Carmody SR, Wente SR.; ''mRNA nuclear export at a glance.''; PubMed Europe PMC Scholia
  35. Valadkhan S, Manley JL.; ''Splicing-related catalysis by protein-free snRNAs.''; PubMed Europe PMC Scholia
  36. Kabachinski G, Schwartz TU.; ''The nuclear pore complex--structure and function at a glance.''; PubMed Europe PMC Scholia
  37. Lévesque L, Bor YC, Matzat LH, Jin L, Berberoglu S, Rekosh D, Hammarskjöld ML, Paschal BM.; ''Mutations in tap uncouple RNA export activity from translocation through the nuclear pore complex.''; PubMed Europe PMC Scholia
  38. Ruskin B, Krainer AR, Maniatis T, Green MR.; ''Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.''; PubMed Europe PMC Scholia
  39. Bessonov S, Anokhina M, Krasauskas A, Golas MM, Sander B, Will CL, Urlaub H, Stark H, Lührmann R.; ''Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis.''; PubMed Europe PMC Scholia
  40. Lamond AI, Konarska MM, Grabowski PJ, Sharp PA.; ''Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.''; PubMed Europe PMC Scholia
  41. Teng IF, Wilson SA.; ''Mapping interactions between mRNA export factors in living cells.''; PubMed Europe PMC Scholia
  42. Ori A, Banterle N, Iskar M, Iskar M, Andrés-Pons A, Escher C, Khanh Bui H, Sparks L, Solis-Mezarino V, Rinner O, Bork P, Lemke EA, Beck M.; ''Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines.''; PubMed Europe PMC Scholia
  43. Culjkovic-Kraljacic B, Borden KL.; ''Aiding and abetting cancer: mRNA export and the nuclear pore.''; PubMed Europe PMC Scholia
  44. Shepard J, Reick M, Olson S, Graveley BR.; ''Characterization of U2AF(6), a splicing factor related to U2AF(35).''; PubMed Europe PMC Scholia
  45. Charlesworth A, Meijer HA, de Moor CH.; ''Specificity factors in cytoplasmic polyadenylation.''; PubMed Europe PMC Scholia
  46. Rappsilber J, Ryder U, Lamond AI, Mann M.; ''Large-scale proteomic analysis of the human spliceosome.''; PubMed Europe PMC Scholia
  47. Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI.; ''Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry.''; PubMed Europe PMC Scholia
  48. Brahms H, Meheus L, de Brabandere V, Fischer U, Lührmann R.; ''Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B' and the Sm-like protein LSm4, and their interaction with the SMN protein.''; PubMed Europe PMC Scholia
  49. Wiegand HL, Coburn GA, Zeng Y, Kang Y, Bogerd HP, Cullen BR.; ''Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes.''; PubMed Europe PMC Scholia
  50. Wagner S, Chiosea S, Ivshina M, Nickerson JA.; ''In vitro FRAP reveals the ATP-dependent nuclear mobilization of the exon junction complex protein SRm160.''; PubMed Europe PMC Scholia
  51. Rabut G, Doye V, Ellenberg J.; ''Mapping the dynamic organization of the nuclear pore complex inside single living cells.''; PubMed Europe PMC Scholia
  52. Erkmann JA, Kutay U.; ''Nuclear export of mRNA: from the site of transcription to the cytoplasm.''; PubMed Europe PMC Scholia
  53. Kang Y, Bogerd HP, Yang J, Cullen BR.; ''Analysis of the RNA binding specificity of the human tap protein, a constitutive transport element-specific nuclear RNA export factor.''; PubMed Europe PMC Scholia
  54. Watkins JL, Murphy R, Emtage JL, Wente SR.; ''The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export.''; PubMed Europe PMC Scholia
  55. Schönemann L, Kühn U, Martin G, Schäfer P, Gruber AR, Keller W, Zavolan M, Wahle E.; ''Reconstitution of CPSF active in polyadenylation: recognition of the polyadenylation signal by WDR33.''; PubMed Europe PMC Scholia
  56. Meinel DM, Burkert-Kautzsch C, Kieser A, O'Duibhir E, Siebert M, Mayer A, Cramer P, Söding J, Holstege FC, Sträßer K.; ''Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II.''; PubMed Europe PMC Scholia
  57. Lin-Moshier Y, Sebastian PJ, Higgins L, Sampson ND, Hewitt JE, Marchant JS.; ''Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling.''; PubMed Europe PMC Scholia
  58. Viphakone N, Cumberbatch MG, Livingstone MJ, Heath PR, Dickman MJ, Catto JW, Wilson SA.; ''Luzp4 defines a new mRNA export pathway in cancer cells.''; PubMed Europe PMC Scholia
  59. Valadkhan S, Mohammadi A, Wachtel C, Manley JL.; ''Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.''; PubMed Europe PMC Scholia
  60. Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M.; ''Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.''; PubMed Europe PMC Scholia
  61. Kang Y, Cullen BR.; ''The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences.''; PubMed Europe PMC Scholia
  62. Madan V, Kanojia D, Li J, Okamoto R, Sato-Otsubo A, Kohlmann A, Sanada M, Grossmann V, Sundaresan J, Shiraishi Y, Miyano S, Thol F, Ganser A, Yang H, Haferlach T, Ogawa S, Koeffler HP.; ''Aberrant splicing of U12-type introns is the hallmark of ZRSR2 mutant myelodysplastic syndrome.''; PubMed Europe PMC Scholia
  63. Folco EG, Lee CS, Dufu K, Yamazaki T, Reed R.; ''The proteins PDIP3 and ZC11A associate with the human TREX complex in an ATP-dependent manner and function in mRNA export.''; PubMed Europe PMC Scholia
  64. Hautbergue GM, Hung ML, Golovanov AP, Lian LY, Wilson SA.; ''Mutually exclusive interactions drive handover of mRNA from export adaptors to TAP.''; PubMed Europe PMC Scholia
  65. Hastings ML, Krainer AR.; ''Functions of SR proteins in the U12-dependent AT-AC pre-mRNA splicing pathway.''; PubMed Europe PMC Scholia
  66. Golovanov AP, Hautbergue GM, Tintaru AM, Lian LY, Wilson SA.; ''The solution structure of REF2-I reveals interdomain interactions and regions involved in binding mRNA export factors and RNA.''; PubMed Europe PMC Scholia
  67. Deckert J, Hartmuth K, Boehringer D, Behzadnia N, Will CL, Kastner B, Stark H, Urlaub H, Lührmann R.; ''Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions.''; PubMed Europe PMC Scholia
  68. Yoshimoto R, Kataoka N, Okawa K, Ohno M.; ''Isolation and characterization of post-splicing lariat-intron complexes.''; PubMed Europe PMC Scholia
  69. Box JA, Bunch JT, Tang W, Baumann P.; ''Spliceosomal cleavage generates the 3' end of telomerase RNA.''; PubMed Europe PMC Scholia
  70. Johnson SA, Kim H, Erickson B, Bentley DL.; ''The export factor Yra1 modulates mRNA 3' end processing.''; PubMed Europe PMC Scholia
  71. Bachi A, Braun IC, Rodrigues JP, Panté N, Ribbeck K, von Kobbe C, Kutay U, Wilm M, Görlich D, Carmo-Fonseca M, Izaurralde E.; ''The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.''; PubMed Europe PMC Scholia
  72. Strässer K, Masuda S, Mason P, Pfannstiel J, Oppizzi M, Rodriguez-Navarro S, Rondón AG, Aguilera A, Struhl K, Reed R, Hurt E.; ''TREX is a conserved complex coupling transcription with messenger RNA export.''; PubMed Europe PMC Scholia
  73. Chang CT, Hautbergue GM, Walsh MJ, Viphakone N, van Dijk TB, Philipsen S, Wilson SA.; ''Chtop is a component of the dynamic TREX mRNA export complex.''; PubMed Europe PMC Scholia
  74. Fontoura BM, Blobel G, Matunis MJ.; ''A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96.''; PubMed Europe PMC Scholia
  75. Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ.; ''Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.''; PubMed Europe PMC Scholia
  76. Crisci A, Raleff F, Bagdiul I, Raabe M, Urlaub H, Rain JC, Krämer A.; ''Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins.''; PubMed Europe PMC Scholia
  77. Shi Y, Di Giammartino DC, Taylor D, Sarkeshik A, Rice WJ, Yates JR, Frank J, Manley JL.; ''Molecular architecture of the human pre-mRNA 3' processing complex.''; PubMed Europe PMC Scholia
  78. Takagaki Y, Manley JL.; ''Complex protein interactions within the human polyadenylation machinery identify a novel component.''; PubMed Europe PMC Scholia
  79. Kreivi JP, Lamond AI.; ''RNA splicing: unexpected spliceosome diversity.''; PubMed Europe PMC Scholia
  80. Katahira J.; ''Nuclear export of messenger RNA.''; PubMed Europe PMC Scholia
  81. Rougemaille M, Dieppois G, Kisseleva-Romanova E, Gudipati RK, Lemoine S, Blugeon C, Boulay J, Jensen TH, Stutz F, Devaux F, Libri D.; ''THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association.''; PubMed Europe PMC Scholia
  82. Luna R, Rondón AG, Aguilera A.; ''New clues to understand the role of THO and other functionally related factors in mRNP biogenesis.''; PubMed Europe PMC Scholia
  83. Zaric B, Chami M, Rémigy H, Engel A, Ballmer-Hofer K, Winkler FK, Kambach C.; ''Reconstitution of two recombinant LSm protein complexes reveals aspects of their architecture, assembly, and function.''; PubMed Europe PMC Scholia
  84. Zhao J, Hyman L, Moore C.; ''Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.''; PubMed Europe PMC Scholia
  85. Lejeune F, Ishigaki Y, Li X, Maquat LE.; ''The exon junction complex is detected on CBP80-bound but not eIF4E-bound mRNA in mammalian cells: dynamics of mRNP remodeling.''; PubMed Europe PMC Scholia
  86. Degot S, Le Hir H, Alpy F, Kedinger V, Stoll I, Wendling C, Seraphin B, Rio MC, Tomasetto C.; ''Association of the breast cancer protein MLN51 with the exon junction complex via its speckle localizer and RNA binding module.''; PubMed Europe PMC Scholia
  87. Daguenet E, Baguet A, Degot S, Schmidt U, Alpy F, Wendling C, Spiegelhalter C, Kessler P, Rio MC, Le Hir H, Bertrand E, Tomasetto C.; ''Perispeckles are major assembly sites for the exon junction core complex.''; PubMed Europe PMC Scholia
  88. Braun IC, Herold A, Rode M, Conti E, Izaurralde E.; ''Overexpression of TAP/p15 heterodimers bypasses nuclear retention and stimulates nuclear mRNA export.''; PubMed Europe PMC Scholia
  89. Masuda S, Das R, Cheng H, Hurt E, Dorman N, Reed R.; ''Recruitment of the human TREX complex to mRNA during splicing.''; PubMed Europe PMC Scholia
  90. Harris ME, Böhni R, Schneiderman MH, Ramamurthy L, Schümperli D, Marzluff WF.; ''Regulation of histone mRNA in the unperturbed cell cycle: evidence suggesting control at two posttranscriptional steps.''; PubMed Europe PMC Scholia
  91. Hautbergue GM, Hung ML, Walsh MJ, Snijders AP, Chang CT, Jones R, Ponting CP, Dickman MJ, Wilson SA.; ''UIF, a New mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA.''; PubMed Europe PMC Scholia
  92. Guzik BW, Levesque L, Prasad S, Bor YC, Black BE, Paschal BM, Rekosh D, Hammarskjöld ML.; ''NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells.''; PubMed Europe PMC Scholia
  93. von Moeller H, Lerner R, Ricciardi A, Basquin C, Marzluff WF, Conti E.; ''Structural and biochemical studies of SLIP1-SLBP identify DBP5 and eIF3g as SLIP1-binding proteins.''; PubMed Europe PMC Scholia
  94. Dreyfuss G, Matunis MJ, Piñol-Roma S, Burd CG.; ''hnRNP proteins and the biogenesis of mRNA.''; PubMed Europe PMC Scholia
  95. Rondón AG, Jimeno S, García-Rubio M, Aguilera A.; ''Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation.''; PubMed Europe PMC Scholia
  96. Steckelberg AL, Boehm V, Gromadzka AM, Gehring NH.; ''CWC22 connects pre-mRNA splicing and exon junction complex assembly.''; PubMed Europe PMC Scholia
  97. Gencheva M, Kato M, Newo AN, Lin RJ.; ''Contribution of DEAH-box protein DHX16 in human pre-mRNA splicing.''; PubMed Europe PMC Scholia
  98. Dufu K, Livingstone MJ, Seebacher J, Gygi SP, Wilson SA, Reed R.; ''ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex.''; PubMed Europe PMC Scholia
  99. Lin DH, Stuwe T, Schilbach S, Rundlet EJ, Perriches T, Mobbs G, Fan Y, Thierbach K, Huber FM, Collins LN, Davenport AM, Jeon YE, Hoelz A.; ''Architecture of the symmetric core of the nuclear pore.''; PubMed Europe PMC Scholia
  100. Hartmuth K, Urlaub H, Vornlocher HP, Will CL, Gentzel M, Wilm M, Lührmann R.; ''Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method.''; PubMed Europe PMC Scholia
  101. Grzybowska EA.; ''Human intronless genes: functional groups, associated diseases, evolution, and mRNA processing in absence of splicing.''; PubMed Europe PMC Scholia
  102. Yao C, Choi EA, Weng L, Xie X, Wan J, Xing Y, Moresco JJ, Tu PG, Yates JR, Shi Y.; ''Overlapping and distinct functions of CstF64 and CstF64τ in mammalian mRNA 3' processing.''; PubMed Europe PMC Scholia
  103. Reichert VL, Le Hir H, Jurica MS, Moore MJ.; ''5' exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly.''; PubMed Europe PMC Scholia
  104. Katahira J, Strässer K, Podtelejnikov A, Mann M, Jung JU, Hurt E.; ''The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.''; PubMed Europe PMC Scholia
  105. Kaufmann I, Martin G, Friedlein A, Langen H, Keller W.; ''Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase.''; PubMed Europe PMC Scholia
  106. Wente SR, Rout MP.; ''The nuclear pore complex and nuclear transport.''; PubMed Europe PMC Scholia
  107. Viphakone N, Hautbergue GM, Walsh M, Chang CT, Holland A, Folco EG, Reed R, Wilson SA.; ''TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export.''; PubMed Europe PMC Scholia
  108. Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R, Elbashir S, Tuschl T, Lührmann R.; ''The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome.''; PubMed Europe PMC Scholia
  109. Kosinski J, Mosalaganti S, von Appen A, Teimer R, DiGuilio AL, Wan W, Bui KH, Hagen WJ, Briggs JA, Glavy JS, Hurt E, Beck M.; ''Molecular architecture of the inner ring scaffold of the human nuclear pore complex.''; PubMed Europe PMC Scholia
  110. Behzadnia N, Golas MM, Hartmuth K, Sander B, Kastner B, Deckert J, Dube P, Will CL, Urlaub H, Stark H, Lührmann R.; ''Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes.''; PubMed Europe PMC Scholia
  111. Herold A, Suyama M, Rodrigues JP, Braun IC, Kutay U, Carmo-Fonseca M, Bork P, Izaurralde E.; ''TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture.''; PubMed Europe PMC Scholia
  112. Taniguchi I, Ohno M.; ''ATP-dependent recruitment of export factor Aly/REF onto intronless mRNAs by RNA helicase UAP56.''; PubMed Europe PMC Scholia
  113. Kota KP, Wagner SR, Huerta E, Underwood JM, Nickerson JA.; ''Binding of ATP to UAP56 is necessary for mRNA export.''; PubMed Europe PMC Scholia
  114. Padgett RA, Konarska MM, Grabowski PJ, Hardy SF, Sharp PA.; ''Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors.''; PubMed Europe PMC Scholia
  115. Shen H, Zheng X, Luecke S, Green MR.; ''The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing.''; PubMed Europe PMC Scholia
  116. Wahle E, Rüegsegger U.; ''3'-End processing of pre-mRNA in eukaryotes.''; PubMed Europe PMC Scholia
  117. Grüter P, Tabernero C, von Kobbe C, Schmitt C, Saavedra C, Bachi A, Wilm M, Felber BK, Izaurralde E.; ''TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.''; PubMed Europe PMC Scholia
  118. Bessonov S, Anokhina M, Will CL, Urlaub H, Lührmann R.; ''Isolation of an active step I spliceosome and composition of its RNP core.''; PubMed Europe PMC Scholia
  119. Singh J, Sikand K, Conrad H, Will CL, Komar AA, Shukla GC.; ''U6atac snRNA stem-loop interacts with U12 p65 RNA binding protein and is functionally interchangeable with the U12 apical stem-loop III.''; PubMed Europe PMC Scholia

History

View all...
CompareRevisionActionTimeUserComment
115088view17:03, 25 January 2021ReactomeTeamReactome version 75
113530view12:00, 2 November 2020ReactomeTeamReactome version 74
112728view16:13, 9 October 2020ReactomeTeamReactome version 73
101644view11:51, 1 November 2018ReactomeTeamreactome version 66
101180view21:38, 31 October 2018ReactomeTeamreactome version 65
100706view20:10, 31 October 2018ReactomeTeamreactome version 64
100256view16:56, 31 October 2018ReactomeTeamreactome version 63
99809view15:20, 31 October 2018ReactomeTeamreactome version 62 (2nd attempt)
93917view13:44, 16 August 2017ReactomeTeamreactome version 61
93494view11:25, 9 August 2017ReactomeTeamreactome version 61
88115view10:05, 26 July 2016RyanmillerOntology Term : 'regulatory pathway' added !
86590view09:21, 11 July 2016ReactomeTeamreactome version 56
83367view11:01, 18 November 2015ReactomeTeamVersion54
81532view13:04, 21 August 2015ReactomeTeamVersion53
77003view08:29, 17 July 2014ReactomeTeamFixed remaining interactions
76708view12:07, 16 July 2014ReactomeTeamFixed remaining interactions
76034view10:09, 11 June 2014ReactomeTeamRe-fixing comment source
75743view11:23, 10 June 2014ReactomeTeamReactome 48 Update
75093view14:04, 8 May 2014AnweshaFixing comment source for displaying WikiPathways description
74829view10:05, 30 April 2014ReactomeTeamReactome46
74740view08:49, 30 April 2014ReactomeTeamReactome46
42105view21:57, 4 March 2011MaintBotAutomatic update
39915view05:56, 21 January 2011MaintBotNew pathway

External references

DataNodes

View all...
NameTypeDatabase referenceComment
3' end cleaved,

ligated exon containing complex

ComplexREACT_3092 (Reactome)
3'-polyadenylated,

capped mRNA complex

ComplexREACT_5827 (Reactome)
ASR2B UnknownREACT_5122 (Reactome)
ATAC A Complex ComplexREACT_4037 (Reactome)
ATAC B Complex ComplexREACT_5095 (Reactome)
ATAC C Complex ComplexREACT_4626 (Reactome)
ATAC C Complex with

lariat containing 5'- end cleaved mRNA

ComplexREACT_5134 (Reactome)
ATP Metabolite15422 (ChEBI)
CBP20 ProteinP52298 (UniProt)
CBP80 ProteinQ09161 (UniProt)
CC1.3 protein /3

RRM, RS

UnknownREACT_4848 (Reactome)
CD2BP2 - GYF ProteinO95400 (UniProt)
CF I ComplexREACT_4896 (Reactome)
CF II ComplexREACT_3772 (Reactome)
CPSF ComplexREACT_4530 (Reactome)
Cap Binding

Complex (CBC)

ComplexREACT_3884 (Reactome)
Cleavage and

Polyadenylation Complex

ComplexREACT_3144 (Reactome)
CstF ComplexREACT_3503 (Reactome)
DDX9/NDH II ProteinQ08211 (UniProt)
DnaJ hom.

NP_055602

UnknownREACT_4415 (Reactome)
E1B-AP5

nucleocytoplasmic transport protein

ProteinQ9BUJ2 (UniProt)
Exon Junction

Complex

ComplexREACT_2984 (Reactome)
Export Receptor

bound mature mRNA Complex

ComplexREACT_2455 (Reactome)
Export Receptor

bound mature mRNA Complex

ComplexREACT_2624 (Reactome)
FLJ10839 DNA-

binding protein

ProteinQ8IX12 (UniProt)
FUS - RNA binding

protein/TLS

ProteinP35637 (UniProt)
HuR /3 RRM protein UnknownREACT_5767 (Reactome)
Ligated exon

containing complex

ComplexREACT_5472 (Reactome)
Magoh-Y14 complex ComplexREACT_5524 (Reactome)
Mature Intronless

Transcript Derived Histone mRNA:TAP: Aly/Ref Complex

ComplexREACT_4560 (Reactome)
Mature Intronless

Transcript Derived Histone mRNA:TAP: Aly/Ref complex

ComplexREACT_5697 (Reactome)
Mature Intronless

Transcript Derived mRNA:eIF4E Complex

ComplexREACT_3252 (Reactome)
Mature Intronless

transcript derived Histone mRNA:SLBP: CBP80:CBP20

ComplexREACT_3802 (Reactome)
Mature SLBP

independent Histone mRNA:eIF4E complex

ComplexREACT_3452 (Reactome)
Mature intronless

derived mRNA complex

ComplexREACT_4047 (Reactome)
Mature intronless

derived mRNA:TAP: Aly/Ref complex

ComplexREACT_4662 (Reactome)
Mature intronless

transcript derived Histone mRNA:SLBP: TAP:Aly/Ref complex

ComplexREACT_2791 (Reactome)
Mature intronless

transcript derived Histone mRNA:SLBP: TAP:Aly/Ref complex

ComplexREACT_3824 (Reactome)
Mature intronless

transcript derived Histone mRNA:SLBP: eIF4E Complex

ComplexREACT_2270 (Reactome)
Mature intronless

transcript derived Histone pre-mRNA: CBC complex

ComplexREACT_5592 (Reactome)
Mature mRNP

Complex

ComplexREACT_4851 (Reactome)
NFAR-2 protein UnknownREACT_4072 (Reactome)
NFX.1 ProteinO43831 (UniProt)
Nuclear Pore

Complex (NPC)

ComplexREACT_5542 (Reactome)
Nucleoplasmic

mature intronless derived mRNA:TAP: Aly/Ref complex

ComplexREACT_4247 (Reactome)
PABPN1 ProteinQ86U42 (UniProt)
Poly(A) polymerase

alpha

ProteinP51003 (UniProt)
RBM 5 ProteinP52756 (UniProt)
RNA polymerase II

(phosphorylated): TFIIF complex

ComplexREACT_4593 (Reactome)
RNPS1 ProteinQ15287 (UniProt)
SF2/ASF/SFRS1 ProteinQ07955 (UniProt)
SMC1 (BAA11495) ProteinQ14683 (UniProt)
SMC2 (P-loop, DA-

box)

UnknownREACT_2529 (Reactome)
SPF31 (Dna J) ProteinO75937 (UniProt)
SR 11/ p54 ProteinQ05519 (UniProt)
SR2 / SC35 ProteinQ01130 (UniProt)
SR4 / SRp75 ProteinQ08170 (UniProt)
SR7/ 9G8 protein ProteinQ16629 (UniProt)
SR9 / SRp30 ProteinQ13242 (UniProt)
SRm160 ProteinQ8IYB3 (UniProt)
SRp20 ProteinP84103 (UniProt)
SRp40 ProteinQ13243 (UniProt)
SRp55 ProteinQ13247 (UniProt)
SURP 2, G patch

protein

ProteinQ8IWZ8 (UniProt)
Spliceosomal

Intermediate C Complex

ComplexREACT_5473 (Reactome)
Spliceosomal A

Complex

ComplexREACT_4512 (Reactome)
Spliceosomal Active C

Complex

ComplexREACT_2680 (Reactome)
Spliceosomal B

Complex

ComplexREACT_3078 (Reactome)
Spliceosomal E

Complex

ComplexREACT_4545 (Reactome)
Spliceosomal active C

complex with lariat containing, 5'-end cleaved pre-mRNP: CBC complex

ComplexREACT_5191 (Reactome)
TAP ProteinQ9UBU9 (UniProt)
TAP:3'-

polyadenylated, capped mRNA complex

ComplexREACT_4136 (Reactome)
THOC4(Aly/Ref) ProteinQ86V81 (UniProt)
U1 snRNP ComplexREACT_2910 (Reactome) The U1 snRNP is a particle consisting of the U1 snRNA and Sm core plus unique snRNP polypeptides. The U1-specific polypeptides are 70K, A, and C. The Sm core polypeptides are B, B', D1, D2, D3, E, F, G. The Sm core is a heteroheptamer in the shape of a donut, containing either B or B', which are almost identical to each other.
U11 snRNP ComplexREACT_5751 (Reactome)
U12 snRNP ComplexREACT_3595 (Reactome)
U2 snRNP ComplexREACT_3715 (Reactome)
U2AF 35 kDa

subunit

ProteinQ01081 (UniProt)
U2AF 65 kDa

subunit

ProteinP26368 (UniProt)
U4 ATAC snRNP ComplexREACT_5266 (Reactome)
U4 ATAC:U5:U6

ATAC Complex

ComplexREACT_4539 (Reactome)
U4 snRNP ComplexREACT_4877 (Reactome)
U4:U5:U6 trisnRNP

complex

ComplexREACT_5066 (Reactome)
U5 snRNP ComplexREACT_2482 (Reactome)
U6 ATAC snRNP ComplexREACT_3135 (Reactome)
UBA, Tudor -

FLJ21007

UnknownREACT_3970 (Reactome)
YB-1 ProteinP67809 (UniProt)
capped pre-mRNA:

CBC:RNA Pol II (phosphorylated) complex

ComplexREACT_3243 (Reactome)
capped, methylated

pre-mRNA:CBC Complex

ComplexREACT_3634 (Reactome)
capped, methylated

pre-mRNP:CBC complex

ComplexREACT_2736 (Reactome)
eIF4E ProteinP06730 (UniProt)
hPrp16 ProteinQ92620 (UniProt)
hPrp17 ProteinO60508 (UniProt)
hPrp18 UnknownREACT_3085 (Reactome)
hPrp19 UnknownREACT_3274 (Reactome)
hPrp22 UnknownREACT_3038 (Reactome)
hPrp43 UnknownREACT_5171 (Reactome)
hPrp5 UnknownREACT_3263 (Reactome)
hSLU7 UnknownREACT_5157 (Reactome)
hTra2 UnknownREACT_4242 (Reactome)
hUpf3B ProteinQ9BZI7 (UniProt)
hnRNP A0 ProteinQ13151 (UniProt)
hnRNP A1 ProteinP09651 (UniProt)
hnRNP A2 ProteinP22626 (UniProt)
hnRNP A3 ProteinP51991 (UniProt)
hnRNP C1/C2 ProteinP07910 (UniProt)
hnRNP D0 ProteinQ14103 (UniProt)
hnRNP F ProteinP52597 (UniProt)
hnRNP G ProteinP38159 (UniProt)
hnRNP H ProteinP31943 (UniProt)
hnRNP H' ProteinP55795 (UniProt)
hnRNP I ProteinP26599 (UniProt)
hnRNP K ProteinP61978 (UniProt)
hnRNP L ProteinP14866 (UniProt)
hnRNP M ProteinP52272 (UniProt)
hnRNP R ProteinO43390 (UniProt)
hnRNP U ProteinQ00839 (UniProt)
hnRNP-E1 ProteinQ15365 (UniProt)
hnRNP-E2 ProteinQ15366 (UniProt)
intron-containing

complex

ComplexREACT_3239 (Reactome)
mRNA (N6-

adenosine)- methyltransferase

ComplexREACT_3236 (Reactome)
mRNA 3'-end

cleavage factor

ComplexREACT_2642 (Reactome)
p68 DEAH protein UnknownREACT_4900 (Reactome)
post exon ligation

complex

ComplexREACT_5793 (Reactome)

Annotated Interactions

No annotated interactions

Retrieved from "https://classic.wikipathways.org/index.php/Pathway:WP1889"