In this final phase of mitosis, new membranes are formed around two sets of chromatids and two daughter cells are formed. The chromosomes and the spindle fibers disperse, and the fiber ring around the center of the cell, composed of actin, contracts, pinching the cell into two daughter cells.
Gandhi R, Gillespie PJ, Hirano T.; ''Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase.''; PubMedEurope PMCScholia
Liu X, Zhou T, Kuriyama R, Erikson RL.; ''Molecular interactions of Polo-like-kinase 1 with the mitotic kinesin-like protein CHO1/MKLP-1.''; PubMedEurope PMCScholia
Neef R, Preisinger C, Sutcliffe J, Kopajtich R, Nigg EA, Mayer TU, Barr FA.; ''Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis.''; PubMedEurope PMCScholia
Kueng S, Hegemann B, Peters BH, Lipp JJ, Schleiffer A, Mechtler K, Peters JM.; ''Wapl controls the dynamic association of cohesin with chromatin.''; PubMedEurope PMCScholia
Watrin E, Schleiffer A, Tanaka K, Eisenhaber F, Nasmyth K, Peters JM.; ''Human Scc4 is required for cohesin binding to chromatin, sister-chromatid cohesion, and mitotic progression.''; PubMedEurope PMCScholia
Shintomi K, Hirano T.; ''Releasing cohesin from chromosome arms in early mitosis: opposing actions of Wapl-Pds5 and Sgo1.''; PubMedEurope PMCScholia
DNA synthesis occurs in the S phase, or the synthesis phase, of the cell cycle. The cell duplicates its hereditary material, and two copies of the chromosome are formed. As DNA replication continues, the E type cyclins shared by the G1 and S phases, are destroyed and the levels of the mitotic cyclins rise.
WAPAL (WAPL) and PDS5 proteins (PDS5A and/or PDS5B) are associated with cohesin throughout most of the cell cycle (Kueng et al. 2006, Gandhi et al. 2006). FGF motifs present in the N-terminal region of WAPAL participate in WAPAL binding to PDS5 and cohesin subunits RAD21, STAG1 (SA1) and STAG2 (SA2) (Shintomi and Hirano 2009).
Cohesin, in complex with WAPAL and PDS5 (PDS5A or PDS5B) binds decondensed chromatin in telophase (Kueng et al. 2006). This binding is facilitated by the NIPBL:MAU2 complex, also known as the 'cohesin loading complex'. NIPBL:MAU2 complex is associated with chromatin from telophase until prophase (Watrin et al. 2006) but seems to form a transient rather than stable complex with cohesin.
Original Pathway at Reactome: http://www.reactome.org/PathwayBrowser/#DB=gk_current&FOCUS_SPECIES_ID=48887&FOCUS_PATHWAY_ID=68884
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PDS5 WAPAL NIPBL MAU2
ChromatinPDS5
WAPALAnnotated Interactions
PDS5
WAPAL