Processing of Capped Intron-Containing Pre-mRNA (Homo sapiens)

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ArcPathVisio Brace Ellipse EndoplasmicReticulum GolgiApparatus HexagonPathVisio MimDegradation Mitochondria Octagon PentagonPathVisio Rectangle RoundedRectangle SarcoplasmicReticulum TriangleEquilateralEast TrianglePathVisio none cytosolnucleoplasmcappedpre-mRNA:CBC:RNAPol II(phosphorylated)complexcapped, methylatedpre-mRNA:CBCComplexmRNA(N6-adenosine)-methyltransferaseHNRNPUHNRNPA2B1HNRNPA1HNRNPFPCBP2HNRNPDHNRNPH2HNRNPMPTBP1HNRNPCHNRNPKPCBP1HNRNPLHNRNPA0HNRNPA3HNRNPH1RBMXHNRNPRcapped, methylatedpre-mRNP:CBCcomplexRBM5HNRNPUL1YBX1FUSSUGP1CD2BP2SRSF9U1 snRNPSRRM1RNPS1SRSF6DHX9SRSF7CCAR1SRSF2SRSF1U2AF1ALYREFSRSF5SRSF3U2AF2Spliceosomal EComplexU2 snRNPDHX38DNAJC8CDC40SRSF11SRSF4Spliceosomal AComplexU4:U5:U6 tri-snRNPcomplexSpliceosomal BComplexCleavage andPolyadenylationComplexSpliceosomalIntermediate C(Bact) ComplexU4 snRNPSpliceosomal ActiveC (B*) ComplexUPF3BSpliceosomal activeC complex withlariat containing,5'-end cleavedpre-mRNP:CBCcomplexMagoh-Y14 complexpre-EJC:SpliceosomalC:pre-mRNP:CBCSpliced mRNPRNApolymeraseII(phosphorylated):TFIIF complexintron-containingcomplexU12 snRNPU11 snRNPATAC A ComplexU4 ATAC:U5:U6 ATACComplexATAC B ComplexU4 ATAC snRNPATAC C ComplexATAC C Complex withlariat containing5'-end cleaved mRNApost exon ligationcomplexU5 snRNPU6 ATAC snRNP3' end cleaved,ligated exoncontaining complexCF IICstFCF ImRNA 3'-end cleavagefactorATPpolyadenylated,cappedmRNA:CBC:EJC:TREX:SRSF proteinsPABPN1PAPOLACPSFNXF1:NXT1::polyadenylated, capped mRNA:CBC:EJC:TREX:SRSF proteinshSLU7NPC:NXF1:NXT1:EJC:CBC:mRNANuclear Pore Complex(NPC)NPC:NXF1:NXT1:EJC:CBC:mRNAmRNA:CBC:EJC:POLDIP3PABPN1CPSFPAPOLACstFCF IICF IMatureIntronlesstranscriptderivedHistonemRNA:SLBP:CBP80:CBP20NCBP2MatureintronlesstranscriptderivedHistonemRNA:SLBP:TAP:Aly/Ref complexNCBP1NXF1MatureintronlesstranscriptderivedHistonemRNA:SLBP:TAP:Aly/Ref complexMature intronlesstranscript derivedHistonemRNA:SLBP:eIF4EComplexMature intronlesstranscript derivedHistonepre-mRNA:CBCcomplexMature IntronlessTranscript DerivedHistonemRNA:TAP:Aly/RefcomplexCap Binding Complex(CBC)NXF1ALYREFEIF4ENuclear Pore Complex(NPC)Mature IntronlessTranscript DerivedHistonemRNA:TAP:Aly/RefComplexMature SLBPindependent HistonemRNA:eIF4E complexMature intronlessderived mRNAcomplexNucleoplasmic matureintronless derivedmRNA:TAP:Aly/RefcomplexNXF1CPSFMature intronlessderivedmRNA:TAP:Aly/RefcomplexMature IntronlessTranscript DerivedmRNA:eIF4E ComplexEIF4ENXF1ALYREFPRP19-CDC5L complexSNRNP27SART1LSM2-8 complexUSP39SplicedmRNA:CBC:EJC:TREXNXF1:NXT1DDX39B:ADPPiSARNPTHO complexZC3H11ACHTOPNXF1:NXT1POLDIP3SARNPCHTOPTHO complexZC3H11ADDX5EIF4A3CASC3DHX16GPKOWNuclear Pore Complex(NPC)hSLU7TRA2BSRRTELAVL1ELAVL2ALYREF, FYTTD1,LUZP4ATPALYREF, FYTTD1,LUZP4ATPDDX39A,BNCBP2NCBP1capped pre-mRNAp-S5-POLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2NCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2METTL3METTL14WTAPPCBP1PCBP2PTBP1HNRNPA0HNRNPA1HNRNPA2B1HNRNPA3HNRNPCHNRNPDHNRNPFRBMXHNRNPH1HNRNPH2HNRNPKHNRNPLHNRNPMHNRNPRHNRNPUNCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2U1 snRNASNRNP70SNRPASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3U1snRNP C proteinU1 snRNASNRNP70SNRPASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3U1snRNP C proteinU2AF1U2AF2SRSF2SRSF7SRSF9SRSF5SRSF6SRSF1SRSF3DHX9FUSYBX1SUGP1CCAR1RBM5CD2BP2HNRNPA0HNRNPA1HNRNPA2B1HNRNPA3HNRNPCHNRNPDHNRNPFRBMXHNRNPH1HNRNPH2HNRNPKHNRNPLHNRNPMHNRNPRHNRNPUHNRNPUL1ALYREFSRRM1RNPS1NCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2PCBP1PCBP2PTBP1DDX5SRRTU2 snRNASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3SNRPA1SNRPB2SF3A1SF3A2SF3A3SF3B1SF3B2SF3B3SF3B4SF3B5PHF5ASF3B14U1 snRNASNRNP70SNRPASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3U1snRNP C proteinU2AF1U2AF2SRSF2SRSF7SRSF9SRSF5SRSF6SRSF1SRSF3DHX9FUSYBX1SUGP1CCAR1RBM5CD2BP2HNRNPA0HNRNPA1HNRNPA2B1HNRNPA3HNRNPCHNRNPDHNRNPFRBMXHNRNPH1HNRNPH2HNRNPKHNRNPLHNRNPMHNRNPRHNRNPUHNRNPUL1ALYREFSRRM1RNPS1NCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2PCBP1PCBP2PTBP1DDX5SRRTU2 snRNASNRPA1SNRPB2SF3A1SF3A2SF3A3SF3B1SF3B2SF3B3SF3B4SF3B5PHF5ASF3B14SRSF4SRSF11DHX38CDC40DNAJC8BCAS2CDC5LCTNNBL1CWC15HSPA8PLRG1PRPF19ELAVL1ELAVL2U4 snRNANHP2L1PPIHPRPF3PRPF4PRPF31SNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3U6 snRNALSM2LSM3LSM4LSM5LSM6LSM7LSM8U5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200PRPF8SART1SNRNP27USP39U1 snRNASNRNP70SNRPASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3U1snRNP C proteinU2AF1U2AF2SRSF2SRSF7SRSF9SRSF5SRSF6SRSF1SRSF3DHX9FUSYBX1SUGP1CCAR1RBM5CD2BP2HNRNPA0HNRNPA1HNRNPA2B1HNRNPA3HNRNPCHNRNPDHNRNPFRBMXHNRNPH1HNRNPH2HNRNPKHNRNPLHNRNPMHNRNPRHNRNPUHNRNPUL1ALYREFSRRM1RNPS1NCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2PCBP1PCBP2PTBP1DDX5SRRTU2 snRNASNRPA1SNRPB2SF3A1SF3A2SF3A3SF3B1SF3B2SF3B3SF3B4SF3B5PHF5ASF3B14SRSF4SRSF11DHX38CDC40DNAJC8BCAS2CDC5LCTNNBL1CWC15HSPA8PLRG1PRPF19ELAVL1ELAVL2U4 snRNANHP2L1PPIHPRPF3PRPF4PRPF31U6 snRNALSM2LSM3LSM4LSM5LSM6LSM7LSM8U5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200PRPF8SART1SNRNP27USP39CPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1CSTF1CSTF2CSTF3PAPOLAPABPN1NUDT21CPSF7CF I - 68 kDa subunitCF I - 72 kDa subunitCLP1PCF11U2 snRNASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3SNRPA1SNRPB2SF3A1SF3A2SF3A3SF3B1SF3B2SF3B3SF3B4SF3B5PHF5ASF3B14U5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200NHP2L1LSM2PRPF8U2AF2U2AF1CPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1CSTF1CSTF2CSTF3PAPOLAPABPN1NUDT21CPSF7CF I - 68 kDa subunitCF I - 72 kDa subunitCLP1PCF11SRSF1SRSF2SRSF4SRSF7SRSF9SRSF11SRSF3SRSF5SRSF6hTra2DHX38CDC40YBX1FUSDNAJC8DHX9SUGP1CCAR1RBM5CD2BP2HNRNPUL1ALYREFSRRM1RNPS1NCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2HNRNPA0HNRNPA1HNRNPA2B1HNRNPA3HNRNPCHNRNPDHNRNPFRBMXHNRNPH1HNRNPH2HNRNPKHNRNPLHNRNPMHNRNPRHNRNPUPCBP1PCBP2PTBP1DDX5BCAS2CDC5LCTNNBL1CWC15HSPA8PLRG1PRPF19U6 snRNAEIF4A3DHX16GPKOWSRRTELAVL1ELAVL2U4 snRNANHP2L1PPIHPRPF3PRPF4PRPF31SNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3U2 snRNASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3SNRPA1SNRPB2SF3A1SF3A2SF3A3SF3B1SF3B2SF3B3SF3B4SF3B5PHF5ASF3B14U5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200NHP2L1LSM2PRPF8U2AF2U2AF1CPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1CSTF1CSTF2CSTF3PAPOLAPABPN1NUDT21CPSF7CF I - 68 kDa subunitCF I - 72 kDa subunitCLP1PCF11SRSF1SRSF2SRSF4SRSF7SRSF9SRSF11SRSF3SRSF5SRSF6DHX38CDC40hTra2YBX1FUSDNAJC8DHX9SUGP1CCAR1RBM5CD2BP2HNRNPUL1ALYREFSRRM1RNPS1NCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2HNRNPA0HNRNPA1HNRNPA2B1HNRNPA3HNRNPCHNRNPDHNRNPFRBMXHNRNPH1HNRNPH2HNRNPKHNRNPLHNRNPMHNRNPRHNRNPUPCBP1PCBP2PTBP1DDX5BCAS2CDC5LCTNNBL1CWC15HSPA8PLRG1PRPF19U6 snRNADHX16GPKOWEIF4A3SRRTELAVL1ELAVL2U2 snRNASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3SNRPA1SNRPB2SF3A1SF3A2SF3A3SF3B1SF3B2SF3B3SF3B4SF3B5PHF5ASF3B14U5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200NHP2L1LSM2PRPF8U2AF1U2AF2CPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1CSTF1CSTF2CSTF3PAPOLAPABPN1NUDT21CPSF7CF I - 68 kDa subunitCF I - 72 kDa subunitCLP1PCF11SRSF1SRSF4SRSF6SRSF5SRSF7SRSF2SRSF3SRSF9DHX38SRSF11CDC40YBX1FUSDNAJC8DHX9SUGP1CCAR1RBM5CD2BP2HNRNPUL1NCBP2NCBP1lariat containing 5'-end cleaved mRNAp-S5-POLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2BCAS2CDC5LCTNNBL1CWC15HSPA8PLRG1PRPF19ALYREFRNPS1SRRM1UPF3BDHX16GPKOWEIF4A3U6 snRNAHNRNPA0HNRNPA1HNRNPA2B1HNRNPA3HNRNPCHNRNPDHNRNPFRBMXHNRNPH1HNRNPH2HNRNPKHNRNPLHNRNPMHNRNPRHNRNPUPCBP1PCBP2PTBP1DDX5TRA2BSRRTELAVL1ELAVL2MAGOHRBM8AEIF4A3MAGOHRBM8AALYREFCCAR1CD2BP2CDC40DHX16DHX38DHX9FUSGPKOWHNRNPUL1RBM5RNPS1SRRM1SRSF1SRSF11SRSF2SRSF3SRSF4SRSF5SRSF6SRSF7SRSF9SUGP1U2AF1U2AF2UPF3BYBX1CPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1CSTF1CSTF2CSTF3PAPOLAPABPN1NUDT21CPSF7CF I - 68 kDa subunitCF I - 72 kDa subunitCLP1PCF11BCAS2CDC5LCTNNBL1CWC15HSPA8PLRG1PRPF19p-S5-POLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2U2 snRNASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3SNRPA1SNRPB2SF3A1SF3A2SF3A3SF3B1SF3B2SF3B3SF3B4SF3B5PHF5ASF3B14U5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200NHP2L1LSM2PRPF8U6 snRNANCBP2NCBP1lariat containing 5'-end cleaved mRNADNAJC8HNRNPA0HNRNPA1HNRNPA2B1HNRNPA3HNRNPCHNRNPDHNRNPFRBMXHNRNPH1HNRNPH2HNRNPKHNRNPLHNRNPMHNRNPRHNRNPUPCBP1PCBP2PTBP1DDX5TRA2BSRRTELAVL1ELAVL2mRNA with spliced exonsNCBP2NCBP1CPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1CSTF1CSTF2CSTF3PAPOLAPABPN1NUDT21CPSF7CF I - 68 kDa subunitCF I - 72 kDa subunitCLP1PCF11SRSF1SRSF2SRSF4SRSF7SRSF9SRSF11SRSF3SRSF5SRSF6hSLU7hTra2DHX38CDC40U2AF1U2AF2CASC3EIF4A3MAGOHRBM8AALYREFRNPS1SRRM1UPF3Bp-S5-POLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2excised intronU2 snRNASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3SNRPA1SNRPB2SF3A1SF3A2SF3A3SF3B1SF3B2SF3B3SF3B4SF3B5PHF5ASF3B14U5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200NHP2L1LSM2PRPF8YBX1FUSDNAJC8DHX9RBM5CD2BP2SUGP1CCAR1HNRNPUL1BCAS2CDC5LCTNNBL1CWC15HSPA8PLRG1PRPF19U6 snRNADHX16GPKOWSNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3SF3B1SF3B2SF3B3SF3B4SF3B5SF3B14ZMAT5ZCRB1RNPC3U12 snRNASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3PDCD7SNRNP48SNRNP35SNRNP25YBX1U11 snRNANCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2SNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3PDCD7SNRNP48SNRNP35SNRNP25YBX1U11 snRNASRSF6SRSF2SRSF7SRSF1SF3B1SF3B2SF3B3SF3B4SF3B5SF3B14ZMAT5ZCRB1RNPC3U12 snRNAU5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200NHP2L1LSM2SNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3PRPF8U4atac snRNAU6 ATAC snRNANCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2SNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3PDCD7SNRNP48SNRNP35SNRNP25YBX1U11 snRNASRSF6SRSF2SRSF7SRSF1SF3B1SF3B2SF3B3SF3B4SF3B5SF3B14ZMAT5ZCRB1RNPC3U12 snRNAU5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200NHP2L1LSM2PRPF8U4atac snRNAU6 ATAC snRNASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3U4atac snRNASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3PDCD7SNRNP48SNRNP35SNRNP25YBX1U11 snRNASF3B1SF3B2SF3B3SF3B4SF3B5SF3B14ZMAT5ZCRB1RNPC3U12 snRNANCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2SRSF2SRSF7SRSF6SRSF1U5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200NHP2L1LSM2PRPF8U6 ATAC snRNASNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3PDCD7SNRNP48SNRNP35SNRNP25YBX1U11 snRNASF3B1SF3B2SF3B3SF3B4SF3B5SF3B14ZMAT5ZCRB1RNPC3U12 snRNAU5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200NHP2L1LSM2PRPF8U6 ATAC snRNASRSF1SRSF2SRSF7SRSF6NCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2mRNA with spliced exonsSRSF1SRSF2SRSF7SRSF6NCBP2NCBP1capped, methylated pre-mRNAPOLR2APOLR2JPOLR2IPOLR2BPOLR2DPOLR2GPOLR2CPOLR2HPOLR2KPOLR2EPOLR2FPOLR2LGTF2F1GTF2F2U5 snRNATXNL4ASNRNP40DDX23PRPF6EFTUD2SNRNP200NHP2L1LSM2SNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3PRPF8SNRPD2SNRPESNRPFSNRPG2xMe-SNRPB2xMe-SNRPD12xMe-SNRPD3U6 ATAC snRNA3'-end cleaved mRNA with spliced exonsNCBP2NCBP1SRSF1SRSF11SRSF2SRSF4SRSF7SRSF9SRSF3SRSF5SRSF6DHX38CDC40hSLU7U2AF1U2AF2CPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1PABPN1PAPOLACASC3EIF4A3MAGOHRBM8AALYREFRNPS1SRRM1UPF3BTHOC1THOC2THOC3THOC5THOC6THOC7SARNPATPDDX39ADDX39BCHTOPPOLDIP3ZC3H11AFYTTD1LUZP4CLP1PCF11CSTF1CSTF2CSTF3NUDT21CPSF7CF I - 68 kDa subunitCF I - 72 kDa subunitNUDT21CPSF7CF I - 68 kDa subunitCF I - 72 kDa subunitCLP1PCF113'-polyadenylated, capped pre-mRNASRSF1SRSF4SRSF7SRSF2SRSF11SRSF6SRSF5SRSF3DHX38CDC40U2AF1U2AF2NCBP2NCBP1SRSF9hSLU7CASC3EIF4A3MAGOHRBM8AALYREFRNPS1SRRM1UPF3BTHOC1THOC2THOC3THOC5THOC6THOC7SARNPATPDDX39ADDX39BCHTOPPOLDIP3ZC3H11AFYTTD1LUZP4CPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1NXF1NXT13'-polyadenylated, capped pre-mRNACDC40NCBP2NCBP1DHX38CASC3EIF4A3MAGOHRBM8AALYREFRNPS1SRRM1UPF3BSRSF1SRSF11SRSF2SRSF3SRSF4SRSF5SRSF6SRSF7SRSF9U2AF1U2AF2hSLU7THOC1THOC2THOC3THOC5THOC6THOC7SARNPCHTOPPOLDIP3ZC3H11AFYTTD1LUZP4NCBP2NCBP1NXF1NXT1NUP160NUP133NUP107NUP98-5NUP37NUP62NUPL1-2NUP54Nup45AAASNUP210NUPL2NUP153NUP155NUP188NUP205NUP214NUP35RANBP2NUP43NUP50NUP85NUP88NUP93RAE1TPRSEH1L-2NUP98-4NUP98-3POM121POM121CNDC1CASC3EIF4A3MAGOHRBM8AALYREFRNPS1SRRM1UPF3BPOLDIP33'-polyadenylated, capped pre-mRNANUP160NUP133NUP107NUP98-5NUP37NUP62NUPL1-2NUP54Nup45AAASNUP210NUPL2NUP153NUP155NUP188NUP205NUP214NUP35RANBP2NUP43NUP50NUP85NUP88NUP93RAE1TPRSEH1L-2NUP98-4NUP98-3POM121POM121CNDC1Ceruloplasmin mRNANCBP2NCBP1NUP160NUP133NUP107NUP98-5NUP37NUP62NUPL1-2NUP54Nup45AAASNUP210NUPL2NUP153NUP155NUP188NUP205NUP214NUP35RANBP2NUP43NUP50NUP85NUP88NUP93RAE1TPRSEH1L-2NUP98-4NUP98-3POM121POM121CNDC1NXF1NXT1EIF4A3CASC3RBM8AMAGOHALYREFRNPS1SRRM1UPF3BPOLDIP3Ceruloplasmin mRNANCBP2NCBP1EIF4A3CASC3RBM8AMAGOHALYREFRNPS1SRRM1UPF3BPOLDIP3CPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1CSTF1CSTF2CSTF3CLP1PCF11NUDT21CPSF7CF I - 68 kDa subunitCF I - 72 kDa subunitSLBPMature Intronless transcript derived Histone mRNANCBP1NCBP2SLBPNXF1Mature Intronless transcript derived Histone mRNAALYREFSLBPMature intronless transcript derived Histone mRNANXF1ALYREFSLBPMature intronless transcript derived Histone mRNAEIF4EMature Intronless transcript derived Histone mRNANCBP2NCBP1Mature Intronless transcript derived Histone mRNANXF1ALYREFNCBP2NCBP1NUP160NUP133NUP107NUP98-5NUP37NUP62NUPL1-2NUP54Nup45AAASNUP210NUPL2NUP153NUP155NUP188NUP205NUP214NUP35RANBP2NUP43NUP50NUP85NUP88NUP93RAE1TPRSEH1L-2NUP98-4NUP98-3POM121POM121CNDC1NXF1ALYREFMature intronless transcript derived Histone mRNAEIF4EMature intronless transcript derived Histone mRNACPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1Mature intronless transcript derived mRNANCBP2NCBP1Mature intronless transcript derived mRNANXF1ALYREFCPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1NXF1Mature intronless derived mRNAALYREFEIF4EMature intronless derived mRNABCAS2CDC5LCTNNBL1CWC15HSPA8PLRG1PRPF19LSM2LSM3LSM4LSM5LSM6LSM7LSM8mRNA with spliced exonsNCBP2NCBP1SRSF1SRSF2SRSF4SRSF7SRSF9SRSF11SRSF3SRSF5SRSF6hSLU7hTra2DHX38CDC40U2AF1U2AF2CASC3EIF4A3MAGOHRBM8AALYREFRNPS1SRRM1UPF3BTHOC1THOC2THOC3THOC5THOC6THOC7SARNPATPDDX39ADDX39BCHTOPPOLDIP3ZC3H11AFYTTD1LUZP4CPSF1CPSF2CPSF3CPSF4 - 30 kDa subunitFIP1L1CSTF1CSTF2CSTF3PAPOLAPABPN1NUDT21CPSF7CF I - 68 kDa subunitCF I - 72 kDa subunitCLP1PCF11NXF1NXT1ADPDDX39ADDX39BTHOC1THOC2THOC3THOC5THOC6THOC7NXF1NXT1THOC1THOC2THOC3THOC5THOC6THOC7NUP160NUP133NUP107NUP98-5NUP37NUP62NUPL1-2NUP54Nup45AAASNUP210NUPL2NUP153NUP155NUP188NUP205NUP214NUP35RANBP2NUP43NUP50NUP85NUP88NUP93RAE1TPRSEH1L-2NUP98-4NUP98-3POM121POM121CNDC1ALYREFFYTTD1LUZP4ALYREFFYTTD1LUZP4DDX39ADDX39BName: Processing of Capped Intron-Containing Pre-mRNAOrganism: Homo sapiens


Description

Co-transcriptional pre-mRNA splicing is not obligatory. Pre-mRNA splicing begins co-transcriptionally and often continues post-transcriptionally. Human genes contain an average of nine introns per gene, which cannot serve as splicing substrates until both 5' and 3' ends of each intron are synthesized. Thus the time that it takes for pol II to synthesize each intron defines a minimal time and distance along the gene in which splicing factors can be recruited. The time that it takes for pol II to reach the end of the gene defines the maximal time in which splicing could occur co-transcriptionally. Thus, the kinetics of transcription can affect the kinetics of splicing.Any covalent change in a primary (nascent) mRNA transcript is mRNA Processing. For successful gene expression, the primary mRNA transcript needs to be converted to a mature mRNA prior to its translation into polypeptide. Eucaryotic mRNAs undergo a series of complex processing reactions; these begin on nascent transcripts as soon as a few ribonucleotides have been synthesized during transcription by RNA Polymerase II, through the export of the mature mRNA to the cytoplasm, and culminate with mRNA turnover in the cytoplasm. View original pathway at:Reactome.

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Bibliography

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  107. Viphakone N, Hautbergue GM, Walsh M, Chang CT, Holland A, Folco EG, Reed R, Wilson SA.; ''TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export.''; PubMed Europe PMC Scholia
  108. Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R, Elbashir S, Tuschl T, Lührmann R.; ''The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome.''; PubMed Europe PMC Scholia
  109. Kosinski J, Mosalaganti S, von Appen A, Teimer R, DiGuilio AL, Wan W, Bui KH, Hagen WJ, Briggs JA, Glavy JS, Hurt E, Beck M.; ''Molecular architecture of the inner ring scaffold of the human nuclear pore complex.''; PubMed Europe PMC Scholia
  110. Behzadnia N, Golas MM, Hartmuth K, Sander B, Kastner B, Deckert J, Dube P, Will CL, Urlaub H, Stark H, Lührmann R.; ''Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes.''; PubMed Europe PMC Scholia
  111. Herold A, Suyama M, Rodrigues JP, Braun IC, Kutay U, Carmo-Fonseca M, Bork P, Izaurralde E.; ''TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture.''; PubMed Europe PMC Scholia
  112. Taniguchi I, Ohno M.; ''ATP-dependent recruitment of export factor Aly/REF onto intronless mRNAs by RNA helicase UAP56.''; PubMed Europe PMC Scholia
  113. Kota KP, Wagner SR, Huerta E, Underwood JM, Nickerson JA.; ''Binding of ATP to UAP56 is necessary for mRNA export.''; PubMed Europe PMC Scholia
  114. Padgett RA, Konarska MM, Grabowski PJ, Hardy SF, Sharp PA.; ''Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors.''; PubMed Europe PMC Scholia
  115. Shen H, Zheng X, Luecke S, Green MR.; ''The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing.''; PubMed Europe PMC Scholia
  116. Wahle E, Rüegsegger U.; ''3'-End processing of pre-mRNA in eukaryotes.''; PubMed Europe PMC Scholia
  117. Grüter P, Tabernero C, von Kobbe C, Schmitt C, Saavedra C, Bachi A, Wilm M, Felber BK, Izaurralde E.; ''TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.''; PubMed Europe PMC Scholia
  118. Bessonov S, Anokhina M, Will CL, Urlaub H, Lührmann R.; ''Isolation of an active step I spliceosome and composition of its RNP core.''; PubMed Europe PMC Scholia
  119. Singh J, Sikand K, Conrad H, Will CL, Komar AA, Shukla GC.; ''U6atac snRNA stem-loop interacts with U12 p65 RNA binding protein and is functionally interchangeable with the U12 apical stem-loop III.''; PubMed Europe PMC Scholia

History

View all...
CompareRevisionActionTimeUserComment
115088
Reactome
view17:03, 25 January 2021ReactomeTeamReactome version 75
113530view12:00, 2 November 2020ReactomeTeamReactome version 74
112728view16:13, 9 October 2020ReactomeTeamReactome version 73
101644view11:51, 1 November 2018ReactomeTeamreactome version 66
101180view21:38, 31 October 2018ReactomeTeamreactome version 65
100706view20:10, 31 October 2018ReactomeTeamreactome version 64
100256view16:56, 31 October 2018ReactomeTeamreactome version 63
99809view15:20, 31 October 2018ReactomeTeamreactome version 62 (2nd attempt)
93917view13:44, 16 August 2017ReactomeTeamreactome version 61
93494view11:25, 9 August 2017ReactomeTeamreactome version 61
88115view10:05, 26 July 2016RyanmillerOntology Term : 'regulatory pathway' added !
86590view09:21, 11 July 2016ReactomeTeamreactome version 56
83367view11:01, 18 November 2015ReactomeTeamVersion54
81532view13:04, 21 August 2015ReactomeTeamVersion53
77003view08:29, 17 July 2014ReactomeTeamFixed remaining interactions
76708view12:07, 16 July 2014ReactomeTeamFixed remaining interactions
76034view10:09, 11 June 2014ReactomeTeamRe-fixing comment source
75743view11:23, 10 June 2014ReactomeTeamReactome 48 Update
75093view14:04, 8 May 2014AnweshaFixing comment source for displaying WikiPathways description
74829view10:05, 30 April 2014ReactomeTeamReactome46
74740view08:49, 30 April 2014ReactomeTeamReactome46
42105view21:57, 4 March 2011MaintBotAutomatic update
39915view05:56, 21 January 2011MaintBotNew pathway

External references

DataNodes

View all...
Name  ↓Type  ↓Database reference  ↓Comment  ↓
2xMe-SNRPB ProteinP14678 (Uniprot-TrEMBL)
2xMe-SNRPD1 ProteinP62314 (Uniprot-TrEMBL)
2xMe-SNRPD3 ProteinP62318 (Uniprot-TrEMBL)
3' end cleaved,

ligated exon

containing complex
ComplexR-HSA-72177 (Reactome)
3'-end cleaved mRNA with spliced exons R-NUL-71998 (Reactome)
3'-polyadenylated, capped pre-mRNA R-NUL-72184 (Reactome)
AAAS ProteinQ9NRG9 (Uniprot-TrEMBL)
ADP MetaboliteCHEBI:16761 (ChEBI)
ALYREF ProteinQ86V81 (Uniprot-TrEMBL)
ALYREF, FYTTD1, LUZP4ComplexR-HSA-8852182 (Reactome)
ALYREFProteinQ86V81 (Uniprot-TrEMBL)
ATAC A ComplexComplexR-HSA-77463 (Reactome)
ATAC B ComplexComplexR-HSA-77470 (Reactome)
ATAC C Complex with

lariat containing

5'-end cleaved mRNA
ComplexR-HSA-77475 (Reactome)
ATAC C ComplexComplexR-HSA-77473 (Reactome)
ATP MetaboliteCHEBI:15422 (ChEBI)
ATPMetaboliteCHEBI:15422 (ChEBI)
BCAS2 ProteinO75934 (Uniprot-TrEMBL)
CASC3 ProteinO15234 (Uniprot-TrEMBL)
CASC3ProteinO15234 (Uniprot-TrEMBL)
CCAR1 ProteinQ8IX12 (Uniprot-TrEMBL)
CCAR1ProteinQ8IX12 (Uniprot-TrEMBL)
CD2BP2 ProteinO95400 (Uniprot-TrEMBL)
CD2BP2ProteinO95400 (Uniprot-TrEMBL)
CDC40 ProteinO60508 (Uniprot-TrEMBL)
CDC40ProteinO60508 (Uniprot-TrEMBL)
CDC5L ProteinQ99459 (Uniprot-TrEMBL)
CF I - 68 kDa subunit R-HSA-72013 (Reactome)
CF I - 72 kDa subunit R-HSA-72014 (Reactome)
CF IIComplexR-HSA-72020 (Reactome)
CF IComplexR-HSA-72015 (Reactome)
CHTOP ProteinQ9Y3Y2 (Uniprot-TrEMBL)
CHTOPProteinQ9Y3Y2 (Uniprot-TrEMBL)
CLP1 ProteinQ92989 (Uniprot-TrEMBL)
CPSF1 ProteinQ10570 (Uniprot-TrEMBL)
CPSF2 ProteinQ9P2I0 (Uniprot-TrEMBL)
CPSF3 ProteinQ9UKF6 (Uniprot-TrEMBL)
CPSF4 - 30 kDa subunit R-NUL-71994 (Reactome)
CPSF7 ProteinQ8N684 (Uniprot-TrEMBL)
CPSFComplexR-HSA-71995 (Reactome)
CSTF1 ProteinQ05048 (Uniprot-TrEMBL)
CSTF2 ProteinP33240 (Uniprot-TrEMBL)
CSTF3 ProteinQ12996 (Uniprot-TrEMBL)
CTNNBL1 ProteinQ8WYA6 (Uniprot-TrEMBL)
CWC15 ProteinQ9P013 (Uniprot-TrEMBL)
Cap Binding Complex (CBC)ComplexR-HSA-77088 (Reactome)
Ceruloplasmin mRNA ProteinM13699 (EMBL)
Cleavage and

Polyadenylation

Complex
ComplexR-HSA-72021 (Reactome)
CstFComplexR-HSA-72006 (Reactome)
DDX23 ProteinQ9BUQ8 (Uniprot-TrEMBL)
DDX39A ProteinO00148 (Uniprot-TrEMBL)
DDX39A,BComplexR-HSA-8852231 (Reactome)
DDX39B ProteinQ13838 (Uniprot-TrEMBL)
DDX39B:ADPComplexR-HSA-8849115 (Reactome)
DDX5 ProteinP17844 (Uniprot-TrEMBL)
DDX5ProteinP17844 (Uniprot-TrEMBL)
DHX16 ProteinO60231 (Uniprot-TrEMBL)
DHX16ProteinO60231 (Uniprot-TrEMBL)
DHX38 ProteinQ92620 (Uniprot-TrEMBL)
DHX38ProteinQ92620 (Uniprot-TrEMBL)
DHX9 ProteinQ08211 (Uniprot-TrEMBL)
DHX9ProteinQ08211 (Uniprot-TrEMBL)
DNAJC8 ProteinO75937 (Uniprot-TrEMBL)
DNAJC8ProteinO75937 (Uniprot-TrEMBL)
EFTUD2 ProteinQ15029 (Uniprot-TrEMBL)
EIF4A3 ProteinP38919 (Uniprot-TrEMBL)
EIF4A3ProteinP38919 (Uniprot-TrEMBL)
EIF4E ProteinP06730 (Uniprot-TrEMBL)
EIF4EProteinP06730 (Uniprot-TrEMBL)
ELAVL1 ProteinQ15717 (Uniprot-TrEMBL)
ELAVL1ProteinQ15717 (Uniprot-TrEMBL)
ELAVL2 ProteinQ12926 (Uniprot-TrEMBL)
ELAVL2ProteinQ12926 (Uniprot-TrEMBL)
FIP1L1 ProteinQ6UN15 (Uniprot-TrEMBL)
FUS ProteinP35637 (Uniprot-TrEMBL)
FUSProteinP35637 (Uniprot-TrEMBL)
FYTTD1 ProteinQ96QD9 (Uniprot-TrEMBL)
GPKOW ProteinQ92917 (Uniprot-TrEMBL)
GPKOWProteinQ92917 (Uniprot-TrEMBL)
GTF2F1 ProteinP35269 (Uniprot-TrEMBL)
GTF2F2 ProteinP13984 (Uniprot-TrEMBL)
HNRNPA0 ProteinQ13151 (Uniprot-TrEMBL)
HNRNPA0ProteinQ13151 (Uniprot-TrEMBL)
HNRNPA1 ProteinP09651 (Uniprot-TrEMBL)
HNRNPA1ProteinP09651 (Uniprot-TrEMBL)
HNRNPA2B1 ProteinP22626 (Uniprot-TrEMBL)
HNRNPA2B1ProteinP22626 (Uniprot-TrEMBL)
HNRNPA3 ProteinP51991 (Uniprot-TrEMBL)
HNRNPA3ProteinP51991 (Uniprot-TrEMBL)
HNRNPC ProteinP07910 (Uniprot-TrEMBL)
HNRNPCProteinP07910 (Uniprot-TrEMBL)
HNRNPD ProteinQ14103 (Uniprot-TrEMBL)
HNRNPDProteinQ14103 (Uniprot-TrEMBL)
HNRNPF ProteinP52597 (Uniprot-TrEMBL)
HNRNPFProteinP52597 (Uniprot-TrEMBL)
HNRNPH1 ProteinP31943 (Uniprot-TrEMBL)
HNRNPH1ProteinP31943 (Uniprot-TrEMBL)
HNRNPH2 ProteinP55795 (Uniprot-TrEMBL)
HNRNPH2ProteinP55795 (Uniprot-TrEMBL)
HNRNPK ProteinP61978 (Uniprot-TrEMBL)
HNRNPKProteinP61978 (Uniprot-TrEMBL)
HNRNPL ProteinP14866 (Uniprot-TrEMBL)
HNRNPLProteinP14866 (Uniprot-TrEMBL)
HNRNPM ProteinP52272 (Uniprot-TrEMBL)
HNRNPMProteinP52272 (Uniprot-TrEMBL)
HNRNPR ProteinO43390 (Uniprot-TrEMBL)
HNRNPRProteinO43390 (Uniprot-TrEMBL)
HNRNPU ProteinQ00839 (Uniprot-TrEMBL)
HNRNPUL1 ProteinQ9BUJ2 (Uniprot-TrEMBL)
HNRNPUL1ProteinQ9BUJ2 (Uniprot-TrEMBL)
HNRNPUProteinQ00839 (Uniprot-TrEMBL)
HSPA8 ProteinP11142 (Uniprot-TrEMBL)
LSM2 ProteinQ9Y333 (Uniprot-TrEMBL)
LSM2-8 complexComplexR-HSA-6806791 (Reactome)
LSM3 ProteinP62310 (Uniprot-TrEMBL)
LSM4 ProteinQ9Y4Z0 (Uniprot-TrEMBL)
LSM5 ProteinQ9Y4Y9 (Uniprot-TrEMBL)
LSM6 ProteinP62312 (Uniprot-TrEMBL)
LSM7 ProteinQ9UK45 (Uniprot-TrEMBL)
LSM8 ProteinO95777 (Uniprot-TrEMBL)
LUZP4 ProteinQ9P127 (Uniprot-TrEMBL)
MAGOH ProteinP61326 (Uniprot-TrEMBL)
METTL14 ProteinQ9HCE5 (Uniprot-TrEMBL)
METTL3 ProteinQ86U44 (Uniprot-TrEMBL)
Magoh-Y14 complexComplexR-HSA-156657 (Reactome)
Mature

Intronless transcript derived Histone

mRNA:SLBP:CBP80:CBP20
ComplexR-HSA-111682 (Reactome)
Mature

intronless transcript derived Histone

mRNA:SLBP:TAP:Aly/Ref complex
ComplexR-HSA-159045 (Reactome)
Mature

intronless transcript derived Histone

mRNA:SLBP:TAP:Aly/Ref complex
ComplexR-HSA-159047 (Reactome)
Mature Intronless

Transcript Derived Histone mRNA:TAP:Aly/Ref

Complex
ComplexR-HSA-158480 (Reactome)
Mature Intronless

Transcript Derived Histone mRNA:TAP:Aly/Ref

complex
ComplexR-HSA-158479 (Reactome)
Mature Intronless

Transcript Derived

mRNA:eIF4E Complex
ComplexR-HSA-113818 (Reactome)
Mature Intronless transcript derived Histone mRNA R-NUL-111676 (Reactome)
Mature SLBP

independent Histone

mRNA:eIF4E complex
ComplexR-HSA-158501 (Reactome)
Mature intronless

derived mRNA:TAP:Aly/Ref

complex
ComplexR-HSA-158442 (Reactome)
Mature intronless

derived mRNA

complex
ComplexR-HSA-112167 (Reactome)
Mature intronless

transcript derived Histone mRNA:SLBP:eIF4E

Complex
ComplexR-HSA-141614 (Reactome)
Mature intronless

transcript derived Histone pre-mRNA:CBC

complex
ComplexR-HSA-156959 (Reactome)
Mature intronless derived mRNA R-NUL-158443 (Reactome) A mature mRNA that has been 3' cleaved, subsequently polyadenylated, and a m7G 5' cap. This product was derived from an intronless transcript.
Mature intronless transcript derived Histone mRNA R-NUL-113820 (Reactome)
Mature intronless transcript derived mRNA R-NUL-158444 (Reactome) A mature mRNA that has been 3' cleaved, subsequently polyadenylated, and a m7G 5' cap. This product was derived from an intronless transcript.
NCBP1 ProteinQ09161 (Uniprot-TrEMBL)
NCBP1ProteinQ09161 (Uniprot-TrEMBL)
NCBP2 ProteinP52298 (Uniprot-TrEMBL)
NCBP2ProteinP52298 (Uniprot-TrEMBL)
NDC1 ProteinQ9BTX1 (Uniprot-TrEMBL)
NHP2L1 ProteinP55769 (Uniprot-TrEMBL)
NPC:NXF1:NXT1:EJC:CBC:mRNAComplexR-HSA-113815 (Reactome)
NPC:NXF1:NXT1:EJC:CBC:mRNAComplexR-HSA-159259 (Reactome)
NUDT21 ProteinO43809 (Uniprot-TrEMBL)
NUP107 ProteinP57740 (Uniprot-TrEMBL)
NUP133 ProteinQ8WUM0 (Uniprot-TrEMBL)
NUP153 ProteinP49790 (Uniprot-TrEMBL)
NUP155 ProteinO75694 (Uniprot-TrEMBL)
NUP160 ProteinQ12769 (Uniprot-TrEMBL)
NUP188 ProteinQ5SRE5 (Uniprot-TrEMBL)
NUP205 ProteinQ92621 (Uniprot-TrEMBL)
NUP210 ProteinQ8TEM1 (Uniprot-TrEMBL)
NUP214 ProteinP35658 (Uniprot-TrEMBL)
NUP35 ProteinQ8NFH5 (Uniprot-TrEMBL)
NUP37 ProteinQ8NFH4 (Uniprot-TrEMBL)
NUP43 ProteinQ8NFH3 (Uniprot-TrEMBL)
NUP50 ProteinQ9UKX7 (Uniprot-TrEMBL)
NUP54 ProteinQ7Z3B4 (Uniprot-TrEMBL)
NUP62 ProteinP37198 (Uniprot-TrEMBL)
NUP85 ProteinQ9BW27 (Uniprot-TrEMBL)
NUP88 ProteinQ99567 (Uniprot-TrEMBL)
NUP93 ProteinQ8N1F7 (Uniprot-TrEMBL)
NUP98-3 ProteinP52948-3 (Uniprot-TrEMBL)
NUP98-4 ProteinP52948-4 (Uniprot-TrEMBL)
NUP98-5 ProteinP52948-5 (Uniprot-TrEMBL)
NUPL1-2 ProteinQ9BVL2-1 (Uniprot-TrEMBL)
NUPL2 ProteinO15504 (Uniprot-TrEMBL)
NXF1 ProteinQ9UBU9 (Uniprot-TrEMBL)
NXF1:NXT1::polyadenylated, capped mRNA:CBC:EJC:TREX:SRSF proteinsComplexR-HSA-159100 (Reactome)
NXF1:NXT1ComplexR-HSA-8849118 (Reactome)
NXF1:NXT1ComplexR-HSA-8849140 (Reactome)
NXF1ProteinQ9UBU9 (Uniprot-TrEMBL)
NXT1 ProteinQ9UKK6 (Uniprot-TrEMBL)
Nuclear Pore Complex (NPC)ComplexR-HSA-157689 (Reactome)
Nucleoplasmic mature

intronless derived mRNA:TAP:Aly/Ref

complex
ComplexR-HSA-158446 (Reactome)
Nup45 ProteinQ9BVL2-2 (Uniprot-TrEMBL)
PABPN1 ProteinQ86U42 (Uniprot-TrEMBL)
PABPN1ProteinQ86U42 (Uniprot-TrEMBL)
PAPOLA ProteinP51003 (Uniprot-TrEMBL)
PAPOLAProteinP51003 (Uniprot-TrEMBL)
PCBP1 ProteinQ15365 (Uniprot-TrEMBL)
PCBP1ProteinQ15365 (Uniprot-TrEMBL)
PCBP2 ProteinQ15366 (Uniprot-TrEMBL)
PCBP2ProteinQ15366 (Uniprot-TrEMBL)
PCF11 ProteinO94913 (Uniprot-TrEMBL)
PDCD7 ProteinQ8N8D1 (Uniprot-TrEMBL)
PHF5A ProteinQ7RTV0 (Uniprot-TrEMBL)
PLRG1 ProteinO43660 (Uniprot-TrEMBL)
POLDIP3 ProteinQ9BY77 (Uniprot-TrEMBL)
POLDIP3ProteinQ9BY77 (Uniprot-TrEMBL)
POLR2A ProteinP24928 (Uniprot-TrEMBL)
POLR2B ProteinP30876 (Uniprot-TrEMBL)
POLR2C ProteinP19387 (Uniprot-TrEMBL)
POLR2D ProteinO15514 (Uniprot-TrEMBL)
POLR2E ProteinP19388 (Uniprot-TrEMBL)
POLR2F ProteinP61218 (Uniprot-TrEMBL)
POLR2G ProteinP62487 (Uniprot-TrEMBL)
POLR2H ProteinP52434 (Uniprot-TrEMBL)
POLR2I ProteinP36954 (Uniprot-TrEMBL)
POLR2J ProteinP52435 (Uniprot-TrEMBL)
POLR2K ProteinP53803 (Uniprot-TrEMBL)
POLR2L ProteinP62875 (Uniprot-TrEMBL)
POM121 ProteinQ96HA1 (Uniprot-TrEMBL)
POM121C ProteinA8CG34 (Uniprot-TrEMBL)
PPIH ProteinO43447 (Uniprot-TrEMBL)
PRP19-CDC5L complexComplexR-HSA-5420896 (Reactome)
PRPF19 ProteinQ9UMS4 (Uniprot-TrEMBL)
PRPF3 ProteinO43395 (Uniprot-TrEMBL)
PRPF31 ProteinQ8WWY3 (Uniprot-TrEMBL)
PRPF4 ProteinO43172 (Uniprot-TrEMBL)
PRPF6 ProteinO94906 (Uniprot-TrEMBL)
PRPF8 ProteinQ6P2Q9 (Uniprot-TrEMBL)
PTBP1 ProteinP26599 (Uniprot-TrEMBL)
PTBP1ProteinP26599 (Uniprot-TrEMBL)
PiMetaboliteCHEBI:18367 (ChEBI)
RAE1 ProteinP78406 (Uniprot-TrEMBL)
RANBP2 ProteinP49792 (Uniprot-TrEMBL)
RBM5 ProteinP52756 (Uniprot-TrEMBL)
RBM5ProteinP52756 (Uniprot-TrEMBL)
RBM8A ProteinQ9Y5S9 (Uniprot-TrEMBL)
RBMX ProteinP38159 (Uniprot-TrEMBL)
RBMXProteinP38159 (Uniprot-TrEMBL)
RNA

polymerase II

(phosphorylated):TFIIF complex
ComplexR-HSA-113404 (Reactome)
RNPC3 ProteinQ96LT9 (Uniprot-TrEMBL)
RNPS1 ProteinQ15287 (Uniprot-TrEMBL)
RNPS1ProteinQ15287 (Uniprot-TrEMBL)
SARNP ProteinP82979 (Uniprot-TrEMBL)
SARNPProteinP82979 (Uniprot-TrEMBL)
SART1 ProteinO43290 (Uniprot-TrEMBL)
SART1ProteinO43290 (Uniprot-TrEMBL)
SEH1L-2 ProteinQ96EE3-2 (Uniprot-TrEMBL)
SF3A1 ProteinQ15459 (Uniprot-TrEMBL)
SF3A2 ProteinQ15428 (Uniprot-TrEMBL)
SF3A3 ProteinQ12874 (Uniprot-TrEMBL)
SF3B1 ProteinO75533 (Uniprot-TrEMBL)
SF3B14 ProteinQ9Y3B4 (Uniprot-TrEMBL)
SF3B2 ProteinQ13435 (Uniprot-TrEMBL)
SF3B3 ProteinQ15393 (Uniprot-TrEMBL)
SF3B4 ProteinQ15427 (Uniprot-TrEMBL)
SF3B5 ProteinQ9BWJ5 (Uniprot-TrEMBL)
SLBP ProteinQ14493 (Uniprot-TrEMBL)
SNRNP200 ProteinO75643 (Uniprot-TrEMBL)
SNRNP25 ProteinQ9BV90 (Uniprot-TrEMBL)
SNRNP27 ProteinQ8WVK2 (Uniprot-TrEMBL)
SNRNP27ProteinQ8WVK2 (Uniprot-TrEMBL)
SNRNP35 ProteinQ16560 (Uniprot-TrEMBL)
SNRNP40 ProteinQ96DI7 (Uniprot-TrEMBL)
SNRNP48 ProteinQ6IEG0 (Uniprot-TrEMBL)
SNRNP70 ProteinP08621 (Uniprot-TrEMBL)
SNRPA ProteinP09012 (Uniprot-TrEMBL)
SNRPA1 ProteinP09661 (Uniprot-TrEMBL)
SNRPB2 ProteinP08579 (Uniprot-TrEMBL)
SNRPD2 ProteinP62316 (Uniprot-TrEMBL)
SNRPE ProteinP62304 (Uniprot-TrEMBL)
SNRPF ProteinP62306 (Uniprot-TrEMBL)
SNRPG ProteinP62308 (Uniprot-TrEMBL)
SRRM1 ProteinQ8IYB3 (Uniprot-TrEMBL)
SRRM1ProteinQ8IYB3 (Uniprot-TrEMBL)
SRRT ProteinQ9BXP5 (Uniprot-TrEMBL)
SRRTProteinQ9BXP5 (Uniprot-TrEMBL)
SRSF1 ProteinQ07955 (Uniprot-TrEMBL)
SRSF11 ProteinQ05519 (Uniprot-TrEMBL)
SRSF11ProteinQ05519 (Uniprot-TrEMBL)
SRSF1ProteinQ07955 (Uniprot-TrEMBL)
SRSF2 ProteinQ01130 (Uniprot-TrEMBL)
SRSF2ProteinQ01130 (Uniprot-TrEMBL)
SRSF3 ProteinP84103 (Uniprot-TrEMBL)
SRSF3ProteinP84103 (Uniprot-TrEMBL)
SRSF4 ProteinQ08170 (Uniprot-TrEMBL)
SRSF4ProteinQ08170 (Uniprot-TrEMBL)
SRSF5 ProteinQ13243 (Uniprot-TrEMBL)
SRSF5ProteinQ13243 (Uniprot-TrEMBL)
SRSF6 ProteinQ13247 (Uniprot-TrEMBL)
SRSF6ProteinQ13247 (Uniprot-TrEMBL)
SRSF7 ProteinQ16629 (Uniprot-TrEMBL)
SRSF7ProteinQ16629 (Uniprot-TrEMBL)
SRSF9 ProteinQ13242 (Uniprot-TrEMBL)
SRSF9ProteinQ13242 (Uniprot-TrEMBL)
SUGP1 ProteinQ8IWZ8 (Uniprot-TrEMBL)
SUGP1ProteinQ8IWZ8 (Uniprot-TrEMBL)
Spliced mRNA:CBC:EJC:TREXComplexR-HSA-8850671 (Reactome)
Spliced mRNPComplexR-HSA-72157 (Reactome)
Spliceosomal

Intermediate C

(Bact) Complex
ComplexR-HSA-72074 (Reactome)
Spliceosomal A ComplexComplexR-HSA-72068 (Reactome)
Spliceosomal Active C (B*) ComplexComplexR-HSA-72022 (Reactome)
Spliceosomal B ComplexComplexR-HSA-72069 (Reactome)
Spliceosomal E ComplexComplexR-HSA-72057 (Reactome)
Spliceosomal active

C complex with lariat containing, 5'-end cleaved pre-mRNP:CBC

complex
ComplexR-HSA-77505 (Reactome)
THO complexComplexR-HSA-8849128 (Reactome)
THOC1 ProteinQ96FV9 (Uniprot-TrEMBL)
THOC2 ProteinQ8NI27 (Uniprot-TrEMBL)
THOC3 ProteinQ96J01 (Uniprot-TrEMBL)
THOC5 ProteinQ13769 (Uniprot-TrEMBL)
THOC6 ProteinQ86W42 (Uniprot-TrEMBL)
THOC7 ProteinQ6I9Y2 (Uniprot-TrEMBL)
TPR ProteinP12270 (Uniprot-TrEMBL)
TRA2B ProteinP62995 (Uniprot-TrEMBL)
TRA2BProteinP62995 (Uniprot-TrEMBL)
TXNL4A ProteinP83876 (Uniprot-TrEMBL)
U1 snRNA ProteinV00590 (EMBL)
U1 snRNPComplexR-HSA-71917 (Reactome) The U1 snRNP is a particle consisting of the U1 snRNA and Sm core plus unique snRNP polypeptides. The U1-specific polypeptides are 70K, A, and C. The Sm core polypeptides are B, B', D1, D2, D3, E, F, G. The Sm core is a heteroheptamer in the shape of a donut, containing either B or B', which are almost identical to each other.
U11 snRNA ProteinENST00000387069 (Ensembl)
U11 snRNPComplexR-HSA-77462 (Reactome)
U12 snRNA ProteinENST00000362512 (Ensembl)
U12 snRNPComplexR-HSA-77472 (Reactome)
U1snRNP C protein R-NUL-71916 (Reactome)
U2 snRNA ProteinX59360 (EMBL)
U2 snRNPComplexR-HSA-71980 (Reactome)
U2AF1 ProteinQ01081 (Uniprot-TrEMBL)
U2AF1ProteinQ01081 (Uniprot-TrEMBL)
U2AF2 ProteinP26368 (Uniprot-TrEMBL)
U2AF2ProteinP26368 (Uniprot-TrEMBL)
U4 ATAC snRNPComplexR-HSA-77465 (Reactome)
U4 ATAC:U5:U6 ATAC ComplexComplexR-HSA-77469 (Reactome)
U4 snRNA ProteinX59361 (EMBL)
U4 snRNPComplexR-HSA-71891 (Reactome)
U4:U5:U6 tri-snRNP complexComplexR-HSA-77506 (Reactome)
U4atac snRNA ProteinENST00000580972 (Ensembl)
U5 snRNA ProteinX04293 (EMBL)
U5 snRNPComplexR-HSA-71981 (Reactome)
U6 ATAC snRNA R-NUL-77466 (Reactome)
U6 ATAC snRNPComplexR-HSA-77467 (Reactome)
U6 snRNA ProteinX59362 (EMBL)
UPF3B ProteinQ9BZI7 (Uniprot-TrEMBL)
UPF3BProteinQ9BZI7 (Uniprot-TrEMBL)
USP39 ProteinQ53GS9 (Uniprot-TrEMBL)
USP39ProteinQ53GS9 (Uniprot-TrEMBL)
WTAP ProteinQ15007 (Uniprot-TrEMBL)
YBX1 ProteinP67809 (Uniprot-TrEMBL)
YBX1ProteinP67809 (Uniprot-TrEMBL)
ZC3H11A ProteinO75152 (Uniprot-TrEMBL)
ZC3H11AProteinO75152 (Uniprot-TrEMBL)
ZCRB1 ProteinQ8TBF4 (Uniprot-TrEMBL)
ZMAT5 ProteinQ9UDW3 (Uniprot-TrEMBL)
capped

pre-mRNA:CBC:RNA Pol II (phosphorylated)

complex
ComplexR-HSA-77089 (Reactome)
capped pre-mRNA R-NUL-72085 (Reactome)
capped, methylated

pre-mRNA:CBC

Complex
ComplexR-HSA-71954 (Reactome)
capped, methylated

pre-mRNP:CBC

complex
ComplexR-HSA-71955 (Reactome)
capped, methylated pre-mRNA R-NUL-77507 (Reactome)
excised intron R-NUL-72158 (Reactome)
hSLU7 ProteinO95391 (Uniprot-TrEMBL)
hSLU7ProteinO95391 (Uniprot-TrEMBL)
hTra2 R-HSA-72063 (Reactome)
intron-containing complexComplexR-HSA-72159 (Reactome)
lariat containing 5'-end cleaved mRNA R-NUL-156756 (Reactome)
mRNA (N6-adenosine)-methyltransferaseComplexR-HSA-72093 (Reactome)
mRNA 3'-end cleavage factorComplexR-HSA-72075 (Reactome)
mRNA with spliced exons R-NUL-72156 (Reactome)
mRNA:CBC:EJC:POLDIP3ComplexR-HSA-159329 (Reactome)
p-S5-POLR2A ProteinP24928 (Uniprot-TrEMBL)
polyadenylated,

capped

mRNA:CBC:EJC:TREX:SRSF proteins
ComplexR-HSA-156769 (Reactome)
post exon ligation complexComplexR-HSA-156556 (Reactome)
pre-EJC:Spliceosomal C:pre-mRNP:CBCComplexR-HSA-156656 (Reactome)

Annotated Interactions

View all...
Source  ↓Target  ↓Type  ↓Database reference  ↓Comment  ↓
3' end cleaved,

ligated exon

containing complex
ArrowR-HSA-72180 (Reactome)
3' end cleaved,

ligated exon

containing complex
R-HSA-72185 (Reactome)
ALYREF, FYTTD1, LUZP4ArrowR-HSA-75096 (Reactome)
ALYREF, FYTTD1, LUZP4R-HSA-8849157 (Reactome)
ALYREFArrowR-HSA-158447 (Reactome)
ALYREFArrowR-HSA-158484 (Reactome)
ALYREFArrowR-HSA-159050 (Reactome)
ALYREFR-HSA-111439 (Reactome)
ALYREFR-HSA-72107 (Reactome)
ALYREFR-HSA-77587 (Reactome)
ALYREFR-HSA-77594 (Reactome)
ATAC A ComplexArrowR-HSA-75080 (Reactome)
ATAC A ComplexR-HSA-75081 (Reactome)
ATAC B ComplexArrowR-HSA-75081 (Reactome)
ATAC B ComplexR-HSA-75079 (Reactome)
ATAC C Complex with

lariat containing

5'-end cleaved mRNA
ArrowR-HSA-75082 (Reactome)
ATAC C Complex with

lariat containing

5'-end cleaved mRNA
R-HSA-75083 (Reactome)
ATAC C ComplexArrowR-HSA-75079 (Reactome)
ATAC C ComplexR-HSA-75082 (Reactome)
ATPR-HSA-72185 (Reactome)
ATPR-HSA-8849157 (Reactome)
CASC3R-HSA-72160 (Reactome)
CCAR1R-HSA-72107 (Reactome)
CD2BP2R-HSA-72107 (Reactome)
CDC40ArrowR-HSA-75096 (Reactome)
CDC40R-HSA-72124 (Reactome)
CF IArrowR-HSA-72180 (Reactome)
CF IIArrowR-HSA-72180 (Reactome)
CF IIR-HSA-72231 (Reactome)
CF IR-HSA-72231 (Reactome)
CHTOPArrowR-HSA-75096 (Reactome)
CHTOPR-HSA-8849157 (Reactome)
CPSFArrowR-HSA-72185 (Reactome)
CPSFArrowR-HSA-77594 (Reactome)
CPSFR-HSA-72231 (Reactome)
Cap Binding Complex (CBC)ArrowR-HSA-111439 (Reactome)
Cap Binding Complex (CBC)ArrowR-HSA-77594 (Reactome)
Cleavage and

Polyadenylation

Complex
ArrowR-HSA-72231 (Reactome)
Cleavage and

Polyadenylation

Complex
R-HSA-72130 (Reactome)
CstFArrowR-HSA-72180 (Reactome)
CstFR-HSA-72231 (Reactome)
DDX39A,BR-HSA-8849157 (Reactome)
DDX39B:ADPArrowR-HSA-159101 (Reactome)
DDX5R-HSA-72107 (Reactome)
DHX16R-HSA-72130 (Reactome)
DHX38ArrowR-HSA-75096 (Reactome)
DHX38R-HSA-72124 (Reactome)
DHX9R-HSA-72107 (Reactome)
DNAJC8R-HSA-72124 (Reactome)
EIF4A3R-HSA-72130 (Reactome)
EIF4ER-HSA-158447 (Reactome)
EIF4ER-HSA-158484 (Reactome)
EIF4ER-HSA-159050 (Reactome)
ELAVL1R-HSA-72124 (Reactome)
ELAVL2R-HSA-72124 (Reactome)
FUSR-HSA-72107 (Reactome)
GPKOWR-HSA-72130 (Reactome)
HNRNPA0ArrowR-HSA-72160 (Reactome)
HNRNPA0R-HSA-72103 (Reactome)
HNRNPA1ArrowR-HSA-72160 (Reactome)
HNRNPA1R-HSA-72103 (Reactome)
HNRNPA2B1ArrowR-HSA-72160 (Reactome)
HNRNPA2B1R-HSA-72103 (Reactome)
HNRNPA3ArrowR-HSA-72160 (Reactome)
HNRNPA3R-HSA-72103 (Reactome)
HNRNPCArrowR-HSA-72160 (Reactome)
HNRNPCR-HSA-72103 (Reactome)
HNRNPDArrowR-HSA-72160 (Reactome)
HNRNPDR-HSA-72103 (Reactome)
HNRNPFArrowR-HSA-72160 (Reactome)
HNRNPFR-HSA-72103 (Reactome)
HNRNPH1ArrowR-HSA-72160 (Reactome)
HNRNPH1R-HSA-72103 (Reactome)
HNRNPH2ArrowR-HSA-72160 (Reactome)
HNRNPH2R-HSA-72103 (Reactome)
HNRNPKArrowR-HSA-72160 (Reactome)
HNRNPKR-HSA-72103 (Reactome)
HNRNPLArrowR-HSA-72160 (Reactome)
HNRNPLR-HSA-72103 (Reactome)
HNRNPMArrowR-HSA-72160 (Reactome)
HNRNPMR-HSA-72103 (Reactome)
HNRNPRArrowR-HSA-72160 (Reactome)
HNRNPRR-HSA-72103 (Reactome)
HNRNPUArrowR-HSA-72160 (Reactome)
HNRNPUL1R-HSA-72107 (Reactome)
HNRNPUR-HSA-72103 (Reactome)
LSM2-8 complexArrowR-HSA-72130 (Reactome)
Magoh-Y14 complexR-HSA-156661 (Reactome)
Mature

Intronless transcript derived Histone

mRNA:SLBP:CBP80:CBP20
R-HSA-77587 (Reactome)
Mature

intronless transcript derived Histone

mRNA:SLBP:TAP:Aly/Ref complex
ArrowR-HSA-159046 (Reactome)
Mature

intronless transcript derived Histone

mRNA:SLBP:TAP:Aly/Ref complex
ArrowR-HSA-77587 (Reactome)
Mature

intronless transcript derived Histone

mRNA:SLBP:TAP:Aly/Ref complex
R-HSA-159046 (Reactome)
Mature

intronless transcript derived Histone

mRNA:SLBP:TAP:Aly/Ref complex
R-HSA-159050 (Reactome)
Mature Intronless

Transcript Derived Histone mRNA:TAP:Aly/Ref

Complex
ArrowR-HSA-158481 (Reactome)
Mature Intronless

Transcript Derived Histone mRNA:TAP:Aly/Ref

Complex
R-HSA-158484 (Reactome)
Mature Intronless

Transcript Derived Histone mRNA:TAP:Aly/Ref

complex
ArrowR-HSA-111439 (Reactome)
Mature Intronless

Transcript Derived Histone mRNA:TAP:Aly/Ref

complex
R-HSA-158481 (Reactome)
Mature Intronless

Transcript Derived

mRNA:eIF4E Complex
ArrowR-HSA-158447 (Reactome)
Mature SLBP

independent Histone

mRNA:eIF4E complex
ArrowR-HSA-158484 (Reactome)
Mature intronless

derived mRNA:TAP:Aly/Ref

complex
ArrowR-HSA-158441 (Reactome)
Mature intronless

derived mRNA:TAP:Aly/Ref

complex
R-HSA-158447 (Reactome)
Mature intronless

derived mRNA

complex
R-HSA-77594 (Reactome)
Mature intronless

transcript derived Histone mRNA:SLBP:eIF4E

Complex
ArrowR-HSA-159050 (Reactome)
Mature intronless

transcript derived Histone pre-mRNA:CBC

complex
R-HSA-111439 (Reactome)
NCBP1ArrowR-HSA-77587 (Reactome)
NCBP2ArrowR-HSA-77587 (Reactome)
NPC:NXF1:NXT1:EJC:CBC:mRNAArrowR-HSA-75096 (Reactome)
NPC:NXF1:NXT1:EJC:CBC:mRNAArrowR-HSA-75097 (Reactome)
NPC:NXF1:NXT1:EJC:CBC:mRNAR-HSA-75097 (Reactome)
NPC:NXF1:NXT1:EJC:CBC:mRNAR-HSA-75098 (Reactome)
NXF1:NXT1::polyadenylated, capped mRNA:CBC:EJC:TREX:SRSF proteinsArrowR-HSA-159101 (Reactome)
NXF1:NXT1::polyadenylated, capped mRNA:CBC:EJC:TREX:SRSF proteinsR-HSA-75096 (Reactome)
NXF1:NXT1ArrowR-HSA-75098 (Reactome)
NXF1:NXT1R-HSA-159101 (Reactome)
NXF1ArrowR-HSA-158447 (Reactome)
NXF1ArrowR-HSA-158484 (Reactome)
NXF1ArrowR-HSA-159050 (Reactome)
NXF1R-HSA-111439 (Reactome)
NXF1R-HSA-77587 (Reactome)
NXF1R-HSA-77594 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-111439 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-158441 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-158447 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-158481 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-158484 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-159046 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-159050 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-75096 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-75097 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-75098 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-77587 (Reactome)
Nuclear Pore Complex (NPC)ArrowR-HSA-77594 (Reactome)
Nuclear Pore Complex (NPC)R-HSA-75096 (Reactome)
Nuclear Pore Complex (NPC)mim-catalysisR-HSA-75097 (Reactome)
Nucleoplasmic mature

intronless derived mRNA:TAP:Aly/Ref

complex
ArrowR-HSA-77594 (Reactome)
Nucleoplasmic mature

intronless derived mRNA:TAP:Aly/Ref

complex
R-HSA-158441 (Reactome)
PABPN1ArrowR-HSA-72185 (Reactome)
PABPN1R-HSA-72231 (Reactome)
PAPOLAArrowR-HSA-72185 (Reactome)
PAPOLAR-HSA-72231 (Reactome)
PAPOLAmim-catalysisR-HSA-72185 (Reactome)
PCBP1ArrowR-HSA-72160 (Reactome)
PCBP1R-HSA-72103 (Reactome)
PCBP2ArrowR-HSA-72160 (Reactome)
PCBP2R-HSA-72103 (Reactome)
POLDIP3R-HSA-8849157 (Reactome)
PRP19-CDC5L complexR-HSA-72124 (Reactome)
PTBP1ArrowR-HSA-72160 (Reactome)
PTBP1R-HSA-72103 (Reactome)
PiArrowR-HSA-159101 (Reactome)
R-HSA-111439 (Reactome) Histone mRNAs are exported by a mechanism that requires TAP, the key factor requires for transport of polyadenylated mRNAs. How TAP is recruited to the histone mRNAs is not known, but it is clear that transport can occur in the absence of either the stemloop or of SLBP. The mature transcript docks at the NPC, in the course of transport CBC will be lost from the mRNA cap, and remain in the nucleous.
R-HSA-156661 (Reactome) At the beginning of this reaction, 1 molecule of 'Magoh-Y14 complex', and 1 molecule of 'Spliceosomal active C complex with lariat containing, 5'-end cleaved pre-mRNP:CBC complex' are present. At the end of this reaction, 1 molecule of 'Exon Junction Complex' is present.

This reaction takes place in the 'nucleoplasm'.

R-HSA-158441 (Reactome) The nucleoplasmic 3' polyadenylated, capped intronless mRNA and TAP are transported through the NPC to the cyotplasmic side of the pore.
R-HSA-158447 (Reactome) The cytoplasmic 3' polyadenylated, capped intronless mRNA and TAP are released from the NPC into the cytosol. Cytosolic TAP will be recycled to the nucleous, while the 3' polyadenylated, capped intronless mRNA is bound by eIF4E and destined for translation.
R-HSA-158481 (Reactome) The mature SLBP independent intronless histone mRNA is transported through the nucler pore to the cytoplasmic side.
R-HSA-158484 (Reactome) At some point eIF4E binds the mature mRNA. While TAP and Aly/Ref are released and will be reycled back to the nucleoplasm.
R-HSA-159046 (Reactome) Once the transport complex is fully assembled the mature mRNA can be translocated from the nucleoplasm to the cytoplasm. The assembled complex starts at the nucleoplasmic basket, travels through the pore, and ends it journey at the cytoplasmic face of the nuclear pore complex.
R-HSA-159050 (Reactome) At some point eIF4E binds the mature mRNA. While TAP and Aly/Ref are released and will be reycled back to the nucleoplasm.
R-HSA-159101 (Reactome) The mRNA is transferred from ALYREF of the TREX complex to NXF1 (TAP) of the NXF1:NXT1 export complex (Hautbergue et al. 2008). Interaction between the TREX complex and NXF1 exposes the arginine-rich RNA-binding domain of NXF1 (Viphakone et al. 2012). Methylation of arginine residues on ALYREF also appears to be necessary for dissociation of mRNA from ALYREF during the transfer (Hung et al. 2010). The interaction between ALYREF and NXF1 occurs in the vicinity of nuclear speckles (Teng and Wilson 2013). DDX39B (UAP56) binds (Kota et al. 2008) and hydrolyzes ATP (Taniguchi and Ohno 2008). UAP56 is believed to hydrolyze ATP and UAP56:ADP is believed to dissociate at some point during the transfer of the mRNA to NXF1 (Taniguchi and Ohno 2008, Chang et al. 2013).
R-HSA-72095 (Reactome) In addition to the methylation of the 5'-cap, there is methylation of internal nucleotides in the mRNA. This methylation can occur in translated and untranslated regions. One to three methyl groups have been seen per mRNA molecule, but methylation is non-stoichiometric. The most frequent methylation observed is at the N6 position of adenosine. The function of mRNA internal methylation, if any, is unknown.
R-HSA-72103 (Reactome) After the nascent pre-mRNA undergoes the initial capping and methylation reactions, it gets associated with numerous factors, including the various heterogeneous nuclear ribonucleoproteins (hnRNPS), the nuclear Cap-Binding Complex, and many splicing factors that make the pre-mRNA a substrate for splicing, 3'-end processing, and in some cases editing.
R-HSA-72107 (Reactome) Pre-mRNA transcripts become rapidly associated with many RNA-binding proteins, including hnRNP proteins, cap-binding proteins, SR proteins, etc; in the test tube this binding does not require splice sites or ATP. The E complex, or early complex, is the first detectable functional intermediate in spliceosome assembly in vitro. It is an ATP-independent complex. When a functional 5' splice site is present, it is bound by the U1 snRNP. The splicing factor U2AF (65 and 35 kDa subunits) binds to the polypyrimidine tract (Y)n and the AG dinucleotide at the 3' splice site, respectively. SF1/mBBP binds to the branch site. Binding of many of these factors is cooperative; e.g., SR proteins and U2AF apparently interact with each other, facilitating their binding to the pre-mRNA. In the presence of ATP, the E complex is converted to the first ATP-dependent spliceosomal complex, namely the A complex.
R-HSA-72124 (Reactome) The A complex is the first ATP-dependent complex in spliceosome assembly. U2AF recruits the U2 snRNP to bind to the branch site in the E complex in an ATP-dependent fashion, to form the A complex. The U2 snRNA base-pairs with the branch site, causing the branch-site adenosine to bulge out, which later positions it for nucleophilic attack at the 5' splice site. The A complex serves as a substrate for formation of the B complex.
R-HSA-72127 (Reactome) The formation of the B complex is ATP-dependent, and both the 5' and 3' splice sites are essential for B complex assembly. The U4 and U6 snRNPS are extensively base-paired, and this U4:U6 complex associates with the U5 snRNP to form a tri-snRNP particle. This tri-snRNP particle then binds to the spliceosomal A complex, to form the spliceosomal B complex.
R-HSA-72130 (Reactome) The intermediate spliceosomal C complex (also called the Bact or B(act) complex) is a very short-lived intermediate; the splicing intermediates are rapidly converted to splicing products. Also, the spliced products are released very rapidly, and no complex containing both the splicing products has been isolated. Conversion of the spliceosomal B complex to the spliceosomal C complex requires ATP. The extensive base-pairing between the U4 and U6 snRNAs is disrupted during the formation of the C complex, which is thought to require helicase-type activity associated with the DEAD box factors. The U4 snRNP and U1 snRNP dissociate from the complex and the LSM2-6 complex of the U6 snRNP is lost, apparently allowing the U6 snRNA to then base-pair with the U2 snRNA and the 5' end of the splice site on the mRNA (Bessonov et al. 2010).
R-HSA-72139 (Reactome) The active C complex is formed due to a conformational change in the intermediate C complex. After formation of the active C complex, the splicing reactions occur very rapidly.
R-HSA-72143 (Reactome) In the first catalytic step of mRNA splicing, the 2' OH group of the bulged A at the branch site performs a nucleophilic attack on the 5' splice site phosphodiester bond, resulting in cleavage of the bond between the 5' exon and the 5' end of the intron, and formation of a new bond between the 5' end of the intron and the branch site A. This results in a lariat-shaped intermediate, with the intron still attached to the 3' exon. The branch site A has a 2'-5' phosphodiester bond with the G at the beginning of the intron, in addition to the usual 5'-3' and 3'-5'phosphodiester bonds.
R-HSA-72160 (Reactome) The second step of the splicing reaction results in cleavage of the transcript at the 3'splice site, and results in ligation of the two exons and excision of the intron.
R-HSA-72180 (Reactome) Endonucleolytic cleavage separates the pre-mRNA into an upstream fragment destined to become the mature mRNA, and a downstream fragment that is rapidly degraded. Cleavage depends on two signals in the RNA, a highly conserved hexanucleotide, AAUAAA, 10 to 30 nucleotides upstream of the cleavage site, and a poorly conserved GU- or U-rich downstream element. Additional sequences, often upstream of AAUAAA, can enhance the efficiency of the reaction. Cleavage occurs most often after a CA dinucleotide. A single gene can have more than one 3' processing site.

Cleavage is preceded by the assembly of a large processing complex, the composition of which is poorly defined. ATP, but not its hydrolysis, is required for assembly. Cleavage at the 3'-end of mRNAs depends on a number of protein factors. CPSF, a heterotetramer, binds specifically to the AAUAAA sequence. The heterotrimer CstF binds the downstream element. CF I, which appears to be composed of two subunits, one of several related larger polypeptides and a common smaller one, also binds RNA, but with unknown specificity. RNA recognition by these proteins is cooperative. Cleavage also requires CF II, composed of at least two subunits, and poly(A) polymerase, the enzyme synthesizing the poly(A) tail in the second step of the reaction. The polypeptide catalyzing the hydrolysis of the phosphodiester bond remains to be identified.

Cleavage produces a 3'-OH on the upstream fragment and a 5'-phosphate on the downstream fragment. At some unknown point after cleavage, the downstream RNA fragment, CstF, CF I and CF II are thought to be released, whereas CPSF and poly(A) polymerase remain to carry out polyadenylation.

R-HSA-72185 (Reactome) The upstream fragment generated by 3' cleavage of the pre-mRNA receives a poly(A) tail of approximately 250 AMP residues in a reaction depending on the AAUAAA sequence 10 to 30 nucleotides upstream of the 3' end. Polyadenylation is carried out by three proteins: Poly(A) polymerase carries the catalytic activity. The enzyme has no specificity for any particular RNA sequence, and it also has a very low affinity for the RNA.

Under physiological conditions, the activity of poly(A) polymerase thus depends on two auxiliary factors, both of which bind to specific RNA sequences and recruit the enzyme by a direct contact. One of these proteins is the heterotetrameric CPSF, which binds the AAUAAA sequence and is also essential for 3' cleavage. The second is the nuclear poly(A) binding protein (PABPN1), which binds the growing poly(A) tails once this has reached a length of about ten nucleotides. Stimulation of poly(A) polymerase by both proteins is synergistic and results in processive elongation of the RNA, i.e. the polymerase adds AMP residues without dissociating from the RNA. The processive reaction is terminated when the tail has reached a length of about 250 nucleotides.

R-HSA-72231 (Reactome)
R-HSA-75079 (Reactome) At the beginning of this reaction, 1 molecule of 'ATAC B Complex' is present. At the end of this reaction, 1 molecule of 'U4 ATAC snRNP', and 1 molecule of 'ATAC C Complex' are present.

This reaction takes place in the 'nucleus'.

R-HSA-75080 (Reactome) U12-type AT-AC introns are distinguished from the major U2-type introns by the consensus sequences of their highly conserved splicing signals. U12 introns have the 5' ss consensus sequence (G/A)TATCCTTT, the branchpoint sequence TTTCCTTAACT and the 3' ss (C/T)AG. Initial recognition of AT-AC introns involves interaction of U12 snRNP with the branch-point sequence and U11 with the 5' ss. Unlike the major splicing pathway, U11 and U12 are in a complex and interact with the pre-mRNA simultaneously, binding in an ATP-dependent manner as a di-snRNP complex and likely bridging the 5' ss and 3' ss region.

Twenty proteins have been identified in the U11/U12 di-snRNP complex including the snRNP Sm proteins B’, B, D3, D2, D1, E, F, and G which are identical to the major splicing pathway Sm proteins. A U2 snRNP core protein complex, SF3b is also found in the U11/U12 di-snRNP, including p14, a protein that interacts with the branchpoint adenosine.

SR proteins are required for formation of A complex in AT-AC splicing. The same SR proteins involved in splicing of the major introns are also active in splicing of AT-AC introns, though, as in the major pathway, there is substrate specificity.

R-HSA-75081 (Reactome) The U4atac/U6atac enters the spliceosome and U6atac snRNA forms base pairing interactions with the 5' ss and also forms base pairing interactions with U12 and U4atac is partially displaced. U5 snRNP, the only snRNP common to both the major and minor splicing pathways, also joins the spliceosome to form the B complex and interacts with nucleotides within the 3' end of the exon flanking the 5' ss.
R-HSA-75082 (Reactome) At the beginning of this reaction, 1 molecule of 'ATAC C Complex' is present. At the end of this reaction, 1 molecule of 'ATAC C Complex with lariat containing 5'-end cleaved mRNA' is present.

This reaction takes place in the 'nucleus'.

R-HSA-75083 (Reactome) At the beginning of this reaction, 1 molecule of 'ATAC C Complex with lariat containing 5'-end cleaved mRNA' is present. At the end of this reaction, 1 molecule of 'U6 ATAC snRNP', 1 molecule of 'post exon ligation complex', 1 molecule of 'U12 snRNP', 1 molecule of 'U11 snRNP', and 1 molecule of 'U5 snRNP' are present.

This reaction takes place in the 'nucleus'.

R-HSA-75096 (Reactome) At the beginning of this reaction, 1 molecule of 'TAP:3'-polyadenylated, capped mRNA complex' is present. At the end of this reaction, 1 molecule of 'SRp55', 1 molecule of 'U2AF 65 kDa subunit', 1 molecule of 'SR9 / SRp30', 1 molecule of 'hTra2', 1 molecule of 'hPrp16', 1 molecule of 'SR 11/ p54', 1 molecule of 'hPrp22', 1 molecule of 'SRp40', 1 molecule of 'hPrp17', 1 molecule of 'SF2/ASF/SFRS1', 1 molecule of 'hSLU7', 1 molecule of 'Export Receptor bound mature mRNA Complex', 1 molecule of 'U2AF 35 kDa subunit', 1 molecule of 'SR2 / SC35', 1 molecule of 'hPrp43', 1 molecule of 'SRp20', 1 molecule of 'SR7/ 9G8 protein', 1 molecule of 'hPrp18', and 1 molecule of 'SR4 / SRp75' are present.

This reaction takes place in the 'nucleoplasm'.

R-HSA-75097 (Reactome) In this reaction, 1 molecule of 'Export Receptor bound mature mRNA Complex' is translocated from nucleoplasm to cytosol.

This reaction takes place in the 'nuclear envelope'.

R-HSA-75098 (Reactome) At the beginning of this reaction, 1 molecule of and 1 molecule of 'Export Receptor bound mature mRNA Complex' are present. At the end of this reaction, 1 molecule of 'THOC4(Aly/Ref)', 1 molecule of 'Mature mRNP Complex', 1 molecule of 'SRm160', and 1 molecule of 'TAP' are present.

This reaction takes place in the 'cytoplasm'.

R-HSA-77587 (Reactome) Histone mRNAs are exported by a mechanism that requires TAP, the key factor requires for transport of polyadenylated mRNAs. How TAP is recruited to the histone mRNAs is not known, but it is clear that transport can occur in the absence of either the stemloop or of SLBP. The stemloop and SLBP enhance the rate of transport of histone mRNAs in Xenopus oocytes, but are not essential for transport
R-HSA-77594 (Reactome) The polyadenylated, capped transcript and TAP dock at the nucleoplasmic side of the NPC. The Cap Binding Complex (CBC) and CPSF complexes are released back into the nucleoplasm.
R-HSA-8849157 (Reactome) The THO subcomplex of the TREX complex initially interacts with the serine-2,5 phosphorylated C-terminal domain of RNA polymerase II (Strasser et al. 2002, Inferred from yeast in Meinel et al. 2013) then with CBP80 of the cap binding complex (Cheng et al. 2006, Dufu et al. 2010, Chi et al. 2013). A TREX complex binds spliced mRNA near the cap during transcription (Cheng et al. 2006). Recruitment is dependent on splicing of the mRNA (Masuda et al. 2005). THO/TREX is required for efficient mRNP biogenesis and export (reviewed in Luna et al 2012). In yeast, components of the THO/TREX complex also affect transcription and 3' processing of mRNA, (Rondon et al. 2003, Rougemaille et al. 2008, Johnson et al. 2011, reviewed in Katahira 2015), however the human TREX complex does not appear to affect transcription (Masuda et al. 2005). The AREX complex, which contains DDX39A (UHR49) rather than DDX39B (UAP56) appears to perform the same function as TREX in mRNA export, but acts on a different subset of mRNAs (Yamazaki et al. 2010).
RBM5R-HSA-72107 (Reactome)
RBMXArrowR-HSA-72160 (Reactome)
RBMXR-HSA-72103 (Reactome)
RNA

polymerase II

(phosphorylated):TFIIF complex
ArrowR-HSA-72160 (Reactome)
RNPS1R-HSA-72107 (Reactome)
SARNPArrowR-HSA-75096 (Reactome)
SARNPR-HSA-8849157 (Reactome)
SART1ArrowR-HSA-72130 (Reactome)
SNRNP27ArrowR-HSA-72130 (Reactome)
SRRM1R-HSA-72107 (Reactome)
SRRTR-HSA-72107 (Reactome)
SRSF11ArrowR-HSA-75096 (Reactome)
SRSF11R-HSA-72124 (Reactome)
SRSF1ArrowR-HSA-75096 (Reactome)
SRSF1R-HSA-72107 (Reactome)
SRSF1R-HSA-75080 (Reactome)
SRSF2ArrowR-HSA-75096 (Reactome)
SRSF2R-HSA-72107 (Reactome)
SRSF2R-HSA-75080 (Reactome)
SRSF3ArrowR-HSA-75096 (Reactome)
SRSF3R-HSA-72107 (Reactome)
SRSF4ArrowR-HSA-75096 (Reactome)
SRSF4R-HSA-72124 (Reactome)
SRSF5ArrowR-HSA-75096 (Reactome)
SRSF5R-HSA-72107 (Reactome)
SRSF6ArrowR-HSA-75096 (Reactome)
SRSF6R-HSA-72107 (Reactome)
SRSF6R-HSA-75080 (Reactome)
SRSF7ArrowR-HSA-75096 (Reactome)
SRSF7R-HSA-72107 (Reactome)
SRSF7R-HSA-75080 (Reactome)
SRSF9ArrowR-HSA-75096 (Reactome)
SRSF9R-HSA-72107 (Reactome)
SUGP1R-HSA-72107 (Reactome)
Spliced mRNA:CBC:EJC:TREXArrowR-HSA-8849157 (Reactome)
Spliced mRNA:CBC:EJC:TREXR-HSA-72180 (Reactome)
Spliced mRNPArrowR-HSA-72160 (Reactome)
Spliced mRNPR-HSA-8849157 (Reactome)
Spliceosomal

Intermediate C

(Bact) Complex
ArrowR-HSA-72130 (Reactome)
Spliceosomal

Intermediate C

(Bact) Complex
R-HSA-72139 (Reactome)
Spliceosomal

Intermediate C

(Bact) Complex
mim-catalysisR-HSA-72139 (Reactome)
Spliceosomal A ComplexArrowR-HSA-72124 (Reactome)
Spliceosomal A ComplexR-HSA-72127 (Reactome)
Spliceosomal Active C (B*) ComplexArrowR-HSA-72139 (Reactome)
Spliceosomal Active C (B*) ComplexR-HSA-72143 (Reactome)
Spliceosomal B ComplexArrowR-HSA-72127 (Reactome)
Spliceosomal B ComplexR-HSA-72130 (Reactome)
Spliceosomal E ComplexArrowR-HSA-72107 (Reactome)
Spliceosomal E ComplexR-HSA-72124 (Reactome)
Spliceosomal active

C complex with lariat containing, 5'-end cleaved pre-mRNP:CBC

complex
ArrowR-HSA-72143 (Reactome)
Spliceosomal active

C complex with lariat containing, 5'-end cleaved pre-mRNP:CBC

complex
R-HSA-156661 (Reactome)
THO complexArrowR-HSA-75096 (Reactome)
THO complexR-HSA-8849157 (Reactome)
TRA2BR-HSA-72143 (Reactome)
U1 snRNPArrowR-HSA-72130 (Reactome)
U1 snRNPR-HSA-72107 (Reactome)
U11 snRNPArrowR-HSA-75083 (Reactome)
U11 snRNPR-HSA-75080 (Reactome)
U12 snRNPArrowR-HSA-75083 (Reactome)
U12 snRNPR-HSA-75080 (Reactome)
U2 snRNPR-HSA-72124 (Reactome)
U2AF1ArrowR-HSA-75096 (Reactome)
U2AF1R-HSA-72107 (Reactome)
U2AF2ArrowR-HSA-75096 (Reactome)
U2AF2R-HSA-72107 (Reactome)
U4 ATAC snRNPArrowR-HSA-75079 (Reactome)
U4 ATAC:U5:U6 ATAC ComplexR-HSA-75081 (Reactome)
U4 snRNPArrowR-HSA-72130 (Reactome)
U4:U5:U6 tri-snRNP complexR-HSA-72127 (Reactome)
U5 snRNPArrowR-HSA-75083 (Reactome)
U6 ATAC snRNPArrowR-HSA-75083 (Reactome)
UPF3BR-HSA-72143 (Reactome)
USP39ArrowR-HSA-72130 (Reactome)
YBX1R-HSA-72107 (Reactome)
ZC3H11AArrowR-HSA-75096 (Reactome)
ZC3H11AR-HSA-8849157 (Reactome)
capped

pre-mRNA:CBC:RNA Pol II (phosphorylated)

complex
R-HSA-72095 (Reactome)
capped, methylated

pre-mRNA:CBC

Complex
ArrowR-HSA-72095 (Reactome)
capped, methylated

pre-mRNA:CBC

Complex
R-HSA-72103 (Reactome)
capped, methylated

pre-mRNA:CBC

Complex
R-HSA-75080 (Reactome)
capped, methylated

pre-mRNP:CBC

complex
ArrowR-HSA-72103 (Reactome)
capped, methylated

pre-mRNP:CBC

complex
R-HSA-72107 (Reactome)
hSLU7ArrowR-HSA-75096 (Reactome)
hSLU7R-HSA-72160 (Reactome)
intron-containing complexArrowR-HSA-72160 (Reactome)
mRNA (N6-adenosine)-methyltransferasemim-catalysisR-HSA-72095 (Reactome)
mRNA 3'-end cleavage factormim-catalysisR-HSA-72180 (Reactome)
mRNA:CBC:EJC:POLDIP3ArrowR-HSA-75098 (Reactome)
polyadenylated,

capped

mRNA:CBC:EJC:TREX:SRSF proteins
ArrowR-HSA-72185 (Reactome)
polyadenylated,

capped

mRNA:CBC:EJC:TREX:SRSF proteins
R-HSA-159101 (Reactome)
polyadenylated,

capped

mRNA:CBC:EJC:TREX:SRSF proteins
mim-catalysisR-HSA-159101 (Reactome)
post exon ligation complexArrowR-HSA-75083 (Reactome)
pre-EJC:Spliceosomal C:pre-mRNP:CBCArrowR-HSA-156661 (Reactome)
pre-EJC:Spliceosomal C:pre-mRNP:CBCR-HSA-72160 (Reactome)
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