mRNA processing (Homo sapiens)

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1mRNA polyadenylationSR-kinasesU2AFSR protein kinasesA5' G cap3'5'hnRNPs5'SR-phosphatasesexon 23'5'5'SR-proteinsSR-repressor proteinsUGSerine/Argenine Rich Proteins(SR)mRNA cappingPoly(A) polCstF(2)SR protein phosphatases3'Formation of the Spliceosomal A complexCstF(2c)(3)3'snRNP Sm core complexmRNA splice site selectionGUFormation of the Spliceosomal A complexAG5'(2a)Polr2asplicing factor SF3Asplicesome associated helicasesCap BindingLariat Formation and 5'-Splice Site Cleavageexon 2U5YYYYInternal methylation of mRNAFormation of the Spliceosomal B complexU2Poly(A)-binding protein IIFormation of the Spliceosomal C complexCleavage of mRNA at the 3'-endU6 snRNPPoly(A)pb IIYYYYRNA helicase Aexon 2U4 snRNPSR-proteinsexon 2mRNA splicing (major pathway)AGU2AFU4/U6U4AU1 snRNPYYYYdegradedAAAAAAA5'AGCPSFP-3'splicing factor SF3BSpt5Poly(A) polymerase alpha65exon 2(1)Additional FactorsAG35AU2 snRNPexon 15'5'mRNA 3'-end processingright click on blue text for detailed informationPrimary RNA transcripthnRNP regulatorsFormation of the Spliceosomal E complexFormation of the active Spliceosomal C complexexon 2Note: New participating members shown at each stepCleavage at the 3'-Splice-Site and Exon LigationAccessory ProteinsYYYY3'Formation of pre-mRNPsAGpre-mRNASR-binding sites3'exon 1snRNP Sm core complexRNA helicaseMisc.YYYYYYYYCF IPutative Alternative SplicingRegulatorsU5(2b)AUG3'CPSFRnmt (methyltransferase)exon 1Additional Factors3'AAUAAASF3b14biPoU6U4:U5:U6 tri-snRNP complexmRNA Cappingexon 1Additional SR proteinsRngtt (capping enzyme)SR-proteinsPi3'U5 snRNPFormation of pre-mRNPsheterogeneous nuclear ribonucleoproteinsU1 snRNPKinases Phosphorylating RS domains?snRNP Sm core complexU2 snRNPU2 snRNPsU15'CFIAGexon 1intronexon 15'AA3'Formation of the Spliceosomal B complexInternal methylation of mRNA5'Processing of Intron-Containing Pre-mRNAPCACF IISR-binding sitesMethylation of mRNAFormation of the Spliceosomal E complexRNAi pathwayRegulation ofAlternative SplicingCF IIAdditional FactorsRNU2HNRPRSF3B1LSM7SNRPB2DHX9DICER1SNRP70PPM1GPCBP2FUSNCBP2CDC40CLK4CSTF3SF3B4SFRS14SUPT5HSNRPA1RNPC2PRPF18SNRPALSM2SFRS6HRMT1L1WDR57SFRS2PTBP2CLK1SF3A3DDX1SSFA1PRPF8U5 snRNARNGTTCDC40NUDT21HNRPLSFRS10HNRPA1PTBP1SFRS12HNRPDCSTF1NSEP1NXF1SNRPD2SFRS5SNRPD1PRPF4HNRPH1CUGBP1HRMT1L2DHX16RNMTCUGBP2CPSF4U4 snRNAPAPOLAHNRPUTMP21C20orf14U2AF1RBMXFUSIP1FUSIP1RBM17PHF5ACPSF1GMPSF3B3CD2BP2HNRPA3P1POLR2AU5-116KDDHX38SF3A2SFRS7HNRPABSFRS8DHX8RNPS1SF4U2AF2DHX15SRPK1XRN2RBM5SRP54SFPQATPSNRPNSFRS4SNRPGSF3B5SPOPSSFA1SNRPEFNBP3SNRPNHNRPA2B1SNRPBHNRPH2PRPF8DNAJC8NCBP1SF3B2SFRS16PSKH1HNRPMSRPK2PRPF4BCLK3DDX20NONOLSM2SMC1L1PRPF4METTL3SRRM1BRUNOL4CPSF3PRPF3CSTF2SFRS1HNRPCSF3A1SFRS9SFRS3SNRPD3PABPN1HEABCLK2TXNL4ASSFA1U1 snRNACSTF2TRnu6CPSF2SNRPFHNRPK


Description

This process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA).

The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' polyadenylation, and RNA splicing, which occur in the cell nucleus before the RNA is translated.

5' Capping: Capping of the pre-mRNA involves the addition of 7-methylguanosine (m7G) to the 5' end. The cap protects the 5' end of the primary RNA transcript from attack by ribonucleases that have specificity to the 3'5' phosphodiester bonds.

3' Processing: The pre-mRNA processing at the 3' end of the RNA molecule involves cleavage of its 3' end and then the addition of about 200 adenine residues to form a poly(A) tail. As the poly(A) tails is synthesised, it binds multiple copies of poly(A) binding protein, which protects the 3'end from ribonuclease digestion.

Splicing: RNA splicing is the process by which introns, regions of RNA that do not code for protein, are removed from the pre-mRNA and the remaining exons connected to re-form a single continuous molecule.

Description adapted from Wikipedia: http://en.wikipedia.org/wiki/Post-transcriptional_modification

Pathway adapted from http://www.reactome.org.

Proteins on this pathway have targeted assays available via the CPTAC Assay Portal.

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Quality Tags

Ontology Terms

 

Bibliography

  1. Carmody SR, Wente SR; ''mRNA nuclear export at a glance.''; J Cell Sci, 2009 PubMed Europe PMC Scholia

History

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CompareRevisionActionTimeUserComment
126308view17:23, 20 April 2023EgonwLicense is CCZero
122796view02:11, 18 May 2022AlexanderPicocleaning up broken comments
117160view09:47, 18 May 2021EweitzModified title
105857view23:19, 15 August 2019KhanspersModified description
102226view21:19, 11 December 2018Khanspersreplaced arcs with curved graphical lines
96893view13:50, 18 April 2018FehrhartFixed minor graphical issues
96288view21:54, 6 March 2018Andrewlmasonnone
95128view15:26, 16 November 2017Mkutmonremoved nested groups
94839view15:55, 6 October 2017Khanspersconverted interactions to graphical lines
79980view14:12, 29 April 2015Zarireplaced new Id for SSFA1
71369view18:37, 17 October 2013MaintBotAutomated update of data sources
70024view01:52, 12 July 2013AlexanderPicoModified title
68926view17:33, 8 July 2013MaintBotUpdated to 2013 gpml schema
67498view11:09, 26 June 2013DdiglesOntology Term : 'spliceosome pathway' added !
45374view21:22, 7 October 2011Khansperscleaned up description and removed old comment
44924view10:51, 6 October 2011MartijnVanIerselOntology Term : 'transcription pathway' added !
41073view23:19, 1 March 2011MaintBotRemoved redundant pathway information and comments
38901view20:24, 24 September 2010AlexanderPicominor tweaks
38872view18:29, 24 September 2010Khanspers
35228view18:39, 11 February 2010KhanspersChanged snRNAs from Other to RNA
35227view18:35, 11 February 2010KhanspersPeriodical save, work in progress
35226view18:21, 11 February 2010KhanspersModified description
35225view18:20, 11 February 2010KhanspersModified description
35122view13:25, 11 February 2010MartijnVanIerseladded lit. ref.
34454view18:24, 10 December 2009MaintBotAutomatic update of empty xrefs
32426view17:10, 15 August 2009MaintBotFixed text labels
32086view13:41, 14 August 2009MaintBotFixed group labels
30272view20:25, 25 July 2009AlexanderPicofixed unsupported characters in TextLabels
26223view23:00, 24 November 2008AlexanderPico
21548view11:32, 14 November 2008MaintBot[[Pathway:Homo sapiens:mRNA processing Reactome]] moved to [[Pathway:WP411]]: Moved to stable identifier
14985view20:16, 24 May 2008AlexanderPicoGrouped and fixed labels
13092view11:15, 17 May 2008MaintBotautomated metabolite conversion
12979view10:59, 17 May 2008MaintBotSticky edges patch by Sjoerd
8274view13:46, 7 January 2008MaintBot[[Pathway:Human:mRNA processing Reactome]] moved to [[Pathway:Homo sapiens:mRNA processing Reactome]]: Renaming species
7757view16:15, 18 December 2007MaintBotfixed category names
7319view12:42, 4 November 2007MaintBotAdded categories to GPML
6407view22:18, 22 May 2007Nsalomonisgpml file for [[Human:mRNA_processing_Reactome]]

External references

DataNodes

View all...
NameTypeDatabase referenceComment
ATPMetabolite1927-31-7 (CAS)
BRUNOL4GeneProduct56853 (Entrez Gene)
C20orf14GeneProduct24148 (Entrez Gene)
CD2BP2GeneProduct10421 (Entrez Gene)
CDC40GeneProduct51362 (Entrez Gene)
CLK1GeneProduct1195 (Entrez Gene)
CLK2GeneProduct1196 (Entrez Gene)
CLK3GeneProduct1198 (Entrez Gene)
CLK4GeneProduct57396 (Entrez Gene)
CPSF1GeneProduct29894 (Entrez Gene)
CPSF2GeneProduct53981 (Entrez Gene)
CPSF3GeneProduct51692 (Entrez Gene)
CPSF4GeneProduct10898 (Entrez Gene)
CSTF1GeneProduct1477 (Entrez Gene)
CSTF2GeneProduct1478 (Entrez Gene)
CSTF2TGeneProduct23283 (Entrez Gene)
CSTF3GeneProduct1479 (Entrez Gene)
CUGBP1GeneProduct10658 (Entrez Gene)
CUGBP2GeneProduct10659 (Entrez Gene)
DDX1GeneProduct1653 (Entrez Gene)
DDX20GeneProduct11218 (Entrez Gene)
DHX15GeneProduct1665 (Entrez Gene)
DHX16GeneProduct8449 (Entrez Gene)
DHX38GeneProduct9785 (Entrez Gene)
DHX8GeneProduct1659 (Entrez Gene)
DHX9GeneProduct1660 (Entrez Gene)
DICER1GeneProduct23405 (Entrez Gene)
DNAJC8GeneProduct22826 (Entrez Gene)
FNBP3GeneProduct55660 (Entrez Gene)
FUSGeneProduct2521 (Entrez Gene)
FUSIP1GeneProduct10772 (Entrez Gene)
GMPMetabolite85-32-5 (CAS)
HEABGeneProduct10978 (Entrez Gene)
HNRPA1GeneProduct3178 (Entrez Gene)
HNRPA2B1GeneProduct3181 (Entrez Gene)
HNRPA3P1GeneProduct10151 (Entrez Gene)
HNRPABGeneProduct3182 (Entrez Gene)
HNRPCGeneProduct3183 (Entrez Gene)
HNRPDGeneProduct3184 (Entrez Gene)
HNRPH1GeneProduct3187 (Entrez Gene)
HNRPH2GeneProduct3188 (Entrez Gene)
HNRPKGeneProduct3190 (Entrez Gene)
HNRPLGeneProduct3191 (Entrez Gene)
HNRPMGeneProduct4670 (Entrez Gene)
HNRPRGeneProduct10236 (Entrez Gene)
HNRPUGeneProduct3192 (Entrez Gene)
HRMT1L1GeneProduct3275 (Entrez Gene)
HRMT1L2GeneProduct3276 (Entrez Gene)
LSM2GeneProduct57819 (Entrez Gene)
LSM7GeneProduct51690 (Entrez Gene)
METTL3GeneProduct56339 (Entrez Gene)
NCBP1GeneProduct4686 (Entrez Gene)
NCBP2GeneProduct22916 (Entrez Gene)
NONOGeneProduct4841 (Entrez Gene)
NSEP1GeneProduct4904 (Entrez Gene)
NUDT21GeneProduct11051 (Entrez Gene)
NXF1GeneProduct10482 (Entrez Gene)
PABPN1GeneProduct8106 (Entrez Gene)
PAPOLAGeneProduct10914 (Entrez Gene)
PCBP2GeneProduct5094 (Entrez Gene)
PHF5AGeneProduct84844 (Entrez Gene)
POLR2AGeneProduct5430 (Entrez Gene)
PPM1GGeneProduct5496 (Entrez Gene) PMID: 12773558
PRPF18GeneProduct8559 (Entrez Gene)
PRPF3GeneProduct9129 (Entrez Gene)
PRPF4GeneProduct9128 (Entrez Gene)
PRPF4BGeneProduct8899 (Entrez Gene)
PRPF8GeneProduct10594 (Entrez Gene)
PSKH1GeneProduct5681 (Entrez Gene) PMID: 12466556
PTBP1GeneProduct5725 (Entrez Gene)
PTBP2GeneProduct58155 (Entrez Gene)
RBM17GeneProduct84991 (Entrez Gene)
RBM5GeneProduct10181 (Entrez Gene)
RBMXGeneProduct27316 (Entrez Gene)
RNGTTGeneProduct8732 (Entrez Gene)
RNMTGeneProduct8731 (Entrez Gene)
RNPC2GeneProduct9584 (Entrez Gene)
RNPS1GeneProduct10921 (Entrez Gene) PMID: 14729963
RNU2GeneProduct6066 (Entrez Gene) GenBank sequence: K00027
Rnu6RnaGenBank sequence: M10329
SF3A1GeneProduct10291 (Entrez Gene)
SF3A2GeneProduct8175 (Entrez Gene)
SF3A3GeneProduct10946 (Entrez Gene)
SF3B1GeneProduct23451 (Entrez Gene)
SF3B2GeneProduct10992 (Entrez Gene)
SF3B3GeneProduct23450 (Entrez Gene)
SF3B4GeneProduct10262 (Entrez Gene)
SF3B5GeneProduct83443 (Entrez Gene)
SF4GeneProduct57794 (Entrez Gene) inferred from: SURP 2, G patch protein, UniProt:O60378
SFPQGeneProduct6421 (Entrez Gene)
SFRS10GeneProduct6434 (Entrez Gene)
SFRS12GeneProduct140890 (Entrez Gene) PMID: 14559993. SRrp86 is a unique member of the SR protein superfamily containing one RNA recognition motif and two serine-arginine (SR)-rich domains separated by an unusual glutamic acid-lysine (EK)-rich region. Previously, we showed that SRrp86 could regulate alternative splicing by both positively and negatively modulating the activity of other SR proteins and that the unique EK domain could inhibit both constitutive and alternative splicing. These functions were most consistent with the model in which SRrp86 functions by interacting with and thereby modulating the activity of target proteins. To identify the specific proteins that interact with SRrp86, we used a yeast two-hybrid library screen and immunoprecipitation coupled to mass spectrometry. We show that SRrp86 interacts with all of the core SR proteins, as well as a subset of other splicing regulatory proteins, including SAF-B, hnRNP G, YB-1, and p72. In contrast to previous results that showed activation of SRp20 by SRrp86, we now show that SAF-B, hnRNP G, and 9G8 all antagonize the activity of SRrp86. Overall, we conclude that not only does SRrp86 regulate SR protein activity but that it is, in turn, regulated by other splicing factors to control alternative splice site selection.
SFRS14GeneProduct10147 (Entrez Gene)
SFRS16GeneProduct11129 (Entrez Gene)
SFRS1GeneProduct6426 (Entrez Gene) Antagonist splicing factor
SFRS2GeneProduct6427 (Entrez Gene)
SFRS3GeneProduct6428 (Entrez Gene)
SFRS4GeneProduct6429 (Entrez Gene)
SFRS5GeneProduct6430 (Entrez Gene)
SFRS6GeneProduct6431 (Entrez Gene)
SFRS7GeneProduct6432 (Entrez Gene)
SFRS8GeneProduct6433 (Entrez Gene)
SFRS9GeneProduct8683 (Entrez Gene) PMID: 12738786, PMID: 12024014 (associated with hnRNP A1 splicing)
SMC1L1GeneProduct8243 (Entrez Gene)
SNRP70GeneProduct6625 (Entrez Gene)
SNRPA1GeneProduct6627 (Entrez Gene)
SNRPAGeneProduct6626 (Entrez Gene)
SNRPB2GeneProduct6629 (Entrez Gene)
SNRPBGeneProduct6628 (Entrez Gene)
SNRPD1GeneProduct6632 (Entrez Gene)
SNRPD2GeneProduct6633 (Entrez Gene)
SNRPD3GeneProduct6634 (Entrez Gene)
SNRPEGeneProduct6635 (Entrez Gene)
SNRPFGeneProduct6636 (Entrez Gene)
SNRPGGeneProduct6637 (Entrez Gene)
SNRPNGeneProduct6638 (Entrez Gene)
SPOPGeneProduct8405 (Entrez Gene)
SRP54GeneProduct6729 (Entrez Gene) PMID: 14729963
SRPK1GeneProduct6732 (Entrez Gene) PMID: 12773558
SRPK2GeneProduct6733 (Entrez Gene) PMID: 12773558
SRRM1GeneProduct10250 (Entrez Gene)
SSFA1GeneProduct4809 (Entrez Gene)
SUPT5HGeneProduct6829 (Entrez Gene)
TMP21GeneProduct10972 (Entrez Gene)
TXNL4AGeneProduct10907 (Entrez Gene)
U1 snRNARnaGenBank sequence: J00645
U2AF1GeneProduct7307 (Entrez Gene)
U2AF2GeneProduct11338 (Entrez Gene)
U4 snRNARnaGenBank sequence: M10328, M18004
U5 snRNARnaGenBank sequence: AB021173
U5-116KDGeneProduct9343 (Entrez Gene)
WDR57GeneProduct9410 (Entrez Gene)
XRN2GeneProduct22803 (Entrez Gene)

Annotated Interactions

No annotated interactions

Retrieved from "https://classic.wikipathways.org/index.php/Pathway:WP411"
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