COPII-mediated vesicle transport (Homo sapiens)

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8, 5138, 418, 38, 413, 16, 22, 29, 40...1, 3, 4, 9, 20...11, 53532, 15, 18, 21, 24...3213, 14, 26, 28, 30...3, 34, 60, 635, 127, 19, 35, 38, 41...38, 39, 4138, 4132, 465, 45, 52, 53endoplasmic reticulum lumencytosoltransport vesicleendoplasmic reticulum-Golgi intermediate compartment membraneSEC31A p-SEC24C MCFD2 CNIH3 SEC23A TRAPPC4 SEC22B 3x4Hyp-GalHyl-COL7A1 COL7A1 TGFA precursor (Glc)3(GlcNAc)2(Man)9-CTSC 3x4Hyp-5Hyl-COL7A1 TMED2 SEC24A TRAPPC5 SEC23IP USO1 GRIA1 SEC13 SEC24B H2OAREG precursor Glycoproteins with Man8 N-glycans 3x4Hyp-GlcGalHyl-COL7A1 GTP SEC22B SEC31A SEC22A PPP6R1 SEC22C GPI-FOLR1 Factor V precursor 3x4Hyp-3Hyp-COL7A1 SEC24C SEC31A:SEC13:SEC23IP3x4Hyp-3Hyp-COL7A1 TMED10 SEC23A SEC23A 3x4Hyp-5Hyl-COL7A1 NAPA STX5,17SEC24D SEC24D COPIIcargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GTP:USO1 homodimer:GOLGA2:GORASP1SEC24C (Glc)3(GlcNAc)2(Man)9-SERPINA1 SEC22C GlcGalHyl-COL7A1 GDP TGFA precursor LMAN2 BET1:GOSR2:STX5:SCFD1PalmC-YKT6 Factor V precursor GPI-CD59 CNIH1 (Glc)3(GlcNAc)2(Man)9-CTSC GOSR2 RAB1:GTP:TBC1D20Cargo concentrationin the ERGPI-FOLR1 SCFD1p-SEC24COL7A1 SEC24A TMED2 CNIH1 STX5 5Hyl-COL7A1 COPIIcargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GTPRAB1B TMED2 MCFD2 PPP6R3 Factor VIII precursor SEC23A SEC24C 3x4Hyp-GalHyl-COL7A1 AREG precursor SEC22C TMED10 CNIH2 TRAPPC2 3x4Hyp-3Hyp-COL7A1 SEC24C 3x4Hyp-3Hyp-5Hyl-COL7A1 (Glc)3(GlcNAc)2(Man)9-SERPINA1 GalHyl-COL7A1 ADP3x4Hyp-GalHyl-COL7A1 GlcGalHyl-COL7A1 RAB1B TRAPPC5 BET1 GPI-CD59 NSF hexamerTRAPPC1 LMAN2L SEC22A SAR1B Factor VIII precursor LMAN1L BET1 GTP GRIA1 TBC1D20GalHyl-COL7A1 RAB1A SEC23A TGFA precursor TMED10 NSF 3x4Hyp-GalHyl-COL7A1 MCFD2 CNIH3 GTP LMAN2L 3x4Hyp-GlcGalHyl-COL7A1 3x4Hyp-3Hyp-5Hyl-COL7A1 GTP3x4Hyp-COL7A1 SEC24C AREG precursor TRAPPC2 Glycoproteins with Man8 N-glycans SEC24A GPI-CD59 Glycoproteins with Man8 N-glycans 3x4Hyp-3Hyp-COL7A1 NAPB MCFD2 GOSR2 RAB1B GPI-CD59 GRIA1 SEC23A LMAN1L p-SEC24B 3x4Hyp-3Hyp-5Hyl-COL7A1 RAB1A AREG precursor GPI-FOLR1 Glycoproteins with Man8 N-glycans CNIH1 SEC24D Glycoproteins with Man8 N-glycans SEC23A SEC24D TMED2 SEC24C Glycoproteins with Man8 N-glycans SEC22A COL7A1 GORASP1 SEC23A CNIH3 GalHyl-COL7A1 PPP6C 3x4Hyp-3Hyp-5Hyl-COL7A1 TRAPPC3 COL7A1 SEC24C PiSTX5 Factor VIII precursor SEC22A Factor V precursor LMAN2 SEC24D TRAPPC6A SEC24B SEC24C STX17 SEC23A TGFA precursor LMAN1 3x4Hyp-GalHyl-COL7A1 STX5 GRIA1 GOLGA2 GOSR2 LMAN1 TFG (Glc)3(GlcNAc)2(Man)9-CTSZ LMAN2L SEC24D TMED2 LMAN2 SEC31A 5Hyl-COL7A1 SEC24A 3x4Hyp-3Hyp-5Hyl-COL7A1 3x4Hyp-3Hyp-GalHyl-COL7A1 SEC22C TRAPPC3 Factor V precursor SEC24A GRIA1 SAR1B:GDPLMAN2L TRAPPC1 GOSR2 (Glc)3(GlcNAc)2(Man)9-SERPINA1 SEC22C 5Hyl-COL7A1 Factor VIII precursor USO1 GRIA1 CNIH1 3x4Hyp-5Hyl-COL7A1 MCFD2 SEC24D SEC22B STX5 TRAPPC5 TMED2 STX5 SEC16B 3x4Hyp-GalHyl-COL7A1 GalHyl-COL7A1 3x4Hyp-3Hyp-GalHyl-COL7A1 GPI-FOLR1 STX5 CNIH1 COL7A1 3x4Hyp-5Hyl-COL7A1 CNIH2 TMED10 CNIH3 SEC24C 3x4Hyp-3Hyp-GalHyl-COL7A1 PalmC-YKT6 TFG hexamerTRAPPC1 Factor V precursor COPIIcargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREsSEC23A CNIH1 LMAN2 LMAN1 MCFD2 MCFD2 SEC24B 3x4Hyp-COL7A1 SEC24D GPI-CD59 NSF TMED2 SEC24C SEC31A LMAN2 SEC24B GOSR2 ATP(Glc)3(GlcNAc)2(Man)9-CTSC GPI-FOLR1 3x4Hyp-3Hyp-5Hyl-COL7A1 SEC23A 3x4Hyp-3Hyp-GlcGalHyl-COL7A1 SAR1B SEC23A SEC24D (Glc)3(GlcNAc)2(Man)9-CTSC TRAPPC6B NAPG TMED2 TMED10 BET1 ANKRD28 CNIH3 Glycoproteins with Man8 N-glycans Factor VIII precursor SAR1B 3x4Hyp-3Hyp-GalHyl-COL7A1 SEC23A GOLGA2 PalmC-YKT6 STX5 TRAPPC6B TRAPPC2L 5Hyl-COL7A1 3x4Hyp-3Hyp-GlcGalHyl-COL7A1 GOSR2 PiSEC23A GRIA1 GalHyl-COL7A1 TRAPPC6A BET1TGFA precursor 3x4Hyp-3Hyp-GalHyl-COL7A1 Factor VIII precursor nascent procollagen VII vesicle (Glc)3(GlcNAc)2(Man)9-CTSZ (Glc)3(GlcNAc)2(Man)9-CTSZ (Glc)3(GlcNAc)2(Man)9-CTSZ TRAPPC9 3x4Hyp-GlcGalHyl-COL7A1 SEC24A 3x4Hyp-COL7A1 SEC22B GlcGalHyl-COL7A1 GalHyl-COL7A1 COPIIcargo:receptors:SAR1B:GTP:SEC23:SEC24:SEC16A:SEC16B:TFG hexamer(Glc)3(GlcNAc)2(Man)9-CTSZ Glycoproteins with Man8 N-glycans STX17 3x4Hyp-3Hyp-5Hyl-COL7A1 Factor V precursor 3x4Hyp-COL7A1 LMAN2 (Glc)3(GlcNAc)2(Man)9-CTSC SEC16A TRAPPC1 GPI-CD59 SEC23:SEC24NAPG GOSR2 SEC24C SEC24C p-SEC24A RAB1B TMED10 CNIH3 TRAPPC2L RAB1A SEC24C 3x4Hyp-3Hyp-5Hyl-COL7A1 Glycoproteins with Man8 N-glycans SEC24C SEC16B Factor VIII precursor COPIIcargoreceptors:SAR1B:GTP:SEC23:SEC243x4Hyp-5Hyl-COL7A1 3x4Hyp-3Hyp-GalHyl-COL7A1 SEC22B GTPER-to-ERGIC v-SNARES3x4Hyp-3Hyp-5Hyl-COL7A1 SEC22A SAR1B CNIH2 3x4Hyp-GlcGalHyl-COL7A1 SEC23IP RAB1B SEC24C SEC13 RAB1:GDP(Glc)3(GlcNAc)2(Man)9-CTSZ LMAN2L GOSR2 (Glc)3(GlcNAc)2(Man)9-CTSC SEC24A CNIH1 PalmC-YKT6 SEC16A 3x4Hyp-3Hyp-COL7A1 GlcGalHyl-COL7A1 GOSR2 LMAN1L TRAPPC9 RAB1:GDPGlcGalHyl-COL7A1 3x4Hyp-GlcGalHyl-COL7A1 RAB1B GTP 3x4Hyp-3Hyp-GlcGalHyl-COL7A1 3x4Hyp-3Hyp-5Hyl-COL7A1 SEC16A:SEC16BSEC24A (Glc)3(GlcNAc)2(Man)9-SERPINA1 GPI-CD59 SNAPsnascent procollagen VII vesicle 3x4Hyp-3Hyp-GalHyl-COL7A1 SEC22A ATP3x4Hyp-3Hyp-COL7A1 3x4Hyp-COL7A1 USO1 SEC13 3x4Hyp-GalHyl-COL7A1 GPI-FOLR1 LMAN1 GalHyl-COL7A1 SEC24D COL7A1 AREG precursor TRAPPC4 cis-SNARE:NSFhexamer:3xSNAPTRAPPC6A TBC1D20 Factor V precursor 3x4Hyp-3Hyp-GlcGalHyl-COL7A1 LMAN1L SEC24C SEC23A (Glc)3(GlcNAc)2(Man)9-CTSZ TMED2 LMAN1 SEC22B TRAPPC9 PalmC-YKT6 LMAN2 CNIH1 COPIIcargo:receptors:SAR1B:GTP:SEC23:SEC24:SEC16A:SEC16B:TFG hexamer:SEC13:SEC31:SEC23IP:v-SNARESGalHyl-COL7A1 TRAPPC2L LMAN2L TMED2 STX5 cis-SNAREGDP LMAN1 LMAN1L TGFA precursor (Glc)3(GlcNAc)2(Man)9-SERPINA1 CNIH3 TRAPPC5 TMED10 SEC23A p-S742,T747 SEC23ACOPIIcargo:receptorsGOSR2 GPI-CD59 CNIH3 LMAN1L (Glc)3(GlcNAc)2(Man)9-CTSZ 3x4Hyp-GlcGalHyl-COL7A1 SEC24C GlcGalHyl-COL7A1 LMAN2 SEC13 TRAPPC2 TRAPPC10 AREG precursor MCFD2 SEC24B LMAN2L PiSEC24D 3x4Hyp-COL7A1 RAB1A TRAPPC9 GlcGalHyl-COL7A1 3x4Hyp-3Hyp-GlcGalHyl-COL7A1 GRIA1 3x4Hyp-5Hyl-COL7A1 LMAN1 GPI-FOLR1 COPIIcargo:receptors:v:SNAREs:GOLGA2:GORASP1:USO1 homodimerTMED10 GlcGalHyl-COL7A1 STX17 SEC22B AREG precursor nascent procollagen VII vesicle PalmC-YKT6 5Hyl-COL7A1 3x4Hyp-3Hyp-COL7A1 (Glc)3(GlcNAc)2(Man)9-SERPINA1 TRAPPC3 STX5 Factor V precursor LMAN2L nascent procollagen VII vesicle TFG GlcGalHyl-COL7A1 GalHyl-COL7A1 Pi3x4Hyp-3Hyp-COL7A1 CNIH2 SEC24D GRIA1 SEC24C ER-to-ERGIC v-SNARESTRAPPC6B SEC23IP RAB1:GTPSEC22B SEC22A 3x4Hyp-GlcGalHyl-COL7A1 SAR1B LMAN1L SCFD1 RAB1A SAR1BCNIH1 LMAN1 SEC22A GTP SEC24C PalmC-YKT6 3x4Hyp-3Hyp-COL7A1 (Glc)3(GlcNAc)2(Man)9-CTSC SEC24CNIH2 (Glc)3(GlcNAc)2(Man)9-CTSC CSNK1DGDPTRAPPC6B SEC24D GTP CNIH2 PREBSEC24D TRAPPC4 SEC22C 3x4Hyp-3Hyp-GalHyl-COL7A1 TRAPPC10 SEC22B SEC23A LMAN1L TRAPPC3 Factor VIII precursor LMAN2 SEC16A 3x4Hyp-GalHyl-COL7A1 3x4Hyp-5Hyl-COL7A1 3x4Hyp-3Hyp-GlcGalHyl-COL7A1 STX5 (Glc)3(GlcNAc)2(Man)9-SERPINA1 5Hyl-COL7A1 TRAPPC2L GDPSEC24C GTP COPIIcargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GDP3x4Hyp-COL7A1 5Hyl-COL7A1 LMAN2 SEC24D PalmC-YKT6 (Glc)3(GlcNAc)2(Man)9-CTSZ 3x4Hyp-3Hyp-GlcGalHyl-COL7A1 SEC23A (Glc)3(GlcNAc)2(Man)9-CTSC SEC24B CNIH3 SEC23A TRAPPC10 GPI-FOLR1 SEC24D 3x4Hyp-5Hyl-COL7A1 TGFA precursor 3x4Hyp-3Hyp-GalHyl-COL7A1 3x4Hyp-5Hyl-COL7A1 USO1 homodimerSEC23IP COL7A1 CNIH1 3x4Hyp-3Hyp-GalHyl-COL7A1 TFG 5Hyl-COL7A1 CNIH2 GDP GPI-CD59 GDP 3x4Hyp-GlcGalHyl-COL7A1 AREG precursor TMED10 GlcGalHyl-COL7A1 SEC24D RAB1A LMAN1 STX5 Factor V precursor 3x4Hyp-3Hyp-GlcGalHyl-COL7A1 SEC24C AREG precursor GOSR2 LMAN1L STX5 SEC16B SEC24B SEC22C 3x4Hyp-GalHyl-COL7A1 SEC23A AREG precursor GOSR2LMAN2L COL7A1 3x4Hyp-COL7A1 GRIA1 TRAPPC6A 3x4Hyp-3Hyp-COL7A1 SEC13 COL7A1 PalmC-YKT6 SAR1B:GTPLMAN1 SEC22B CNIH3 MCFD2 SEC24B PalmC-YKT6 Factor VIII precursor 3x4Hyp-3Hyp-GlcGalHyl-COL7A1 CNIH2 GPI-FOLR1 SEC22C SEC22A SEC22C (Glc)3(GlcNAc)2(Man)9-SERPINA1 SEC23IP GPI-CD59 SEC24B CNIH2 COL7A1 Glycoproteins with Man8 N-glycans 5Hyl-COL7A1 TRAPPC4 GOLGA2 CNIH2 SEC22C GOSR2 GORASP1 5Hyl-COL7A1 GOSR2 3x4Hyp-COL7A1 Factor VIII precursor LMAN1L SEC22A SEC31A COPIIcargo:receptorsSTX5 3x4Hyp-3Hyp-GlcGalHyl-COL7A1 p-SEC24D SCFD1 TGFA precursor 3x4Hyp-COL7A1 3x4Hyp-5Hyl-COL7A1 TRAPPC2 (Glc)3(GlcNAc)2(Man)9-CTSZ GalHyl-COL7A1 (Glc)3(GlcNAc)2(Man)9-SERPINA1 3x4Hyp-GalHyl-COL7A1 RAB1B SEC23IP SEC24D SEC24D TGFA precursor STX17 SCFD1 PP6(Glc)3(GlcNAc)2(Man)9-SERPINA1 STX5 TGFA precursor 3x4Hyp-GlcGalHyl-COL7A1 LMAN2L SEC24A GORASP1 TMED10 MCFD2 GTP (Glc)3(GlcNAc)2(Man)9-CTSC GPI-FOLR1 TRAPPCIINAPA Factor V precursor GOLGA2:GORASP1SEC31A NAPB TRAPPC10 RAB1A 3x4Hyp-GlcGalHyl-COL7A1 SEC13 6, 10, 17, 23, 27...


Description

COPII components (known as Sec13p, Sec23p, Sec24p, Sec31p, and Sar1p in yeast) traffic cargo from the endoplasmic reticulum to the ER-Golgi intermediate compartment (ERGIC). COPII-coated vesicles were originally discovered in the yeast Saccharomyces cerevisiae using genetic approaches coupled with a cell-free assay. The mammalian counterpart of this pathway is represented here. Newly synthesized proteins destined for secretion are sorted into COPII-coated vesicles at specialized regions of the ER. These vesicles leave the ER, become uncoated and subsequently fuse with the ERGIC membrane. View original pathway at:Reactome.

Comments

Reactome-Converter 
Pathway is converted from Reactome ID: 204005
Reactome-version 
Reactome version: 66
Reactome Author 
Reactome Author: Gillespie, Marc E

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Bibliography

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History

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CompareRevisionActionTimeUserComment
114690view16:16, 25 January 2021ReactomeTeamReactome version 75
113136view11:20, 2 November 2020ReactomeTeamReactome version 74
112367view15:29, 9 October 2020ReactomeTeamReactome version 73
101705view14:44, 1 November 2018DeSlOntology Term : 'transport pathway' added !
101269view11:16, 1 November 2018ReactomeTeamreactome version 66
100807view20:44, 31 October 2018ReactomeTeamreactome version 65
100718view20:11, 31 October 2018ReactomeTeamNew pathway

External references

DataNodes

View all...
NameTypeDatabase referenceComment
(Glc)3(GlcNAc)2(Man)9-CTSC ProteinP53634 (Uniprot-TrEMBL)
(Glc)3(GlcNAc)2(Man)9-CTSZ ProteinQ9UBR2 (Uniprot-TrEMBL)
(Glc)3(GlcNAc)2(Man)9-SERPINA1 ProteinP01009 (Uniprot-TrEMBL)
3x4Hyp-3Hyp-5Hyl-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
3x4Hyp-3Hyp-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
3x4Hyp-3Hyp-GalHyl-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
3x4Hyp-3Hyp-GlcGalHyl-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
3x4Hyp-5Hyl-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
3x4Hyp-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
3x4Hyp-GalHyl-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
3x4Hyp-GlcGalHyl-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
5Hyl-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
ADPMetaboliteCHEBI:16761 (ChEBI)
ANKRD28 ProteinO15084 (Uniprot-TrEMBL)
AREG precursor ProteinP15514 (Uniprot-TrEMBL)
ATPMetaboliteCHEBI:15422 (ChEBI)
BET1 ProteinO15155 (Uniprot-TrEMBL)
BET1:GOSR2:STX5:SCFD1ComplexR-HSA-5694305 (Reactome)
BET1ProteinO15155 (Uniprot-TrEMBL)
CNIH1 ProteinO95406 (Uniprot-TrEMBL)
CNIH2 ProteinQ6PI25 (Uniprot-TrEMBL)
CNIH3 ProteinQ8TBE1 (Uniprot-TrEMBL)
COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
COPII

cargo

receptors:SAR1B:GTP:SEC23:SEC24
ComplexR-HSA-5694523 (Reactome)
COPII cargo:receptors:SAR1B:GTP:SEC23:SEC24:SEC16A:SEC16B:TFG hexamer:SEC13:SEC31:SEC23IP:v-SNARESComplexR-HSA-5694337 (Reactome)
COPII cargo:receptors:SAR1B:GTP:SEC23:SEC24:SEC16A:SEC16B:TFG hexamerComplexR-HSA-5694339 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GDPComplexR-HSA-5694334 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GTP:USO1 homodimer:GOLGA2:GORASP1ComplexR-HSA-5694332 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GTPComplexR-HSA-5694330 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREsComplexR-HSA-5694329 (Reactome)
COPII cargo:receptors:v:SNAREs:GOLGA2:GORASP1:USO1 homodimerComplexR-HSA-5694326 (Reactome)
COPII cargo:receptorsComplexR-HSA-5694244 (Reactome)
COPII cargo:receptorsComplexR-HSA-5694253 (Reactome)
CSNK1DProteinP48730 (Uniprot-TrEMBL)
Cargo concentration in the ERPathwayR-HSA-5694530 (Reactome) Computational analysis suggests that ~25% of the proteome may be exported from the ER in human cells (Kanapin et al, 2003). These cargo need to be recognized and concentrated into COPII vesicles, which range in size from 60-90 nm, and which move cargo from the ER to the ERGIC in mammalian cells (reviewed in Lord et al, 2013; Szul and Sztul, 2011). Recognition of transmembrane cargo is mediated by interaction with one of the 4 isoforms of SEC24, a component of the inner COPII coat (Miller et al, 2002; Miller et al, 2003; Mossessova et al, 2003; Mancias and Goldberg, 2008). Soluble cargo in the ER lumen is concentrated into COPII vesicles through interaction with a receptor of the ERGIC-53 family, the p24 family or the ERV family. Each of these families of transmembrane receptors interact with cargo through their lumenal domains and with components of the COPII coat with their cytoplasmic domains and are packaged into the COPII vesicle along with the cargo. The receptors are subsequently recycled to the ER in COPI vesicles through retrograde traffic (reviewed in Dancourt and Barlowe, 2010). Packaging of large cargo such as fibrillar collagen depends on the transmembrane accessory factors MIA3 (also known as TANGO1) and CTAGE5. Like the ERGIC, p24 and ERV cargo receptors, MIA3 and CTAGE5 interact both with the collagen cargo and with components of the COPII coat. Unlike the other cargo receptors, however, MIA3 and CTAGE5 are not loaded into the vesicle but remain in the ER membrane (reviewed in Malhotra and Erlmann, 2011; Malhotra et al, 2015).
ER-to-ERGIC v-SNARESComplexR-HSA-5694306 (Reactome)
ER-to-ERGIC v-SNARESComplexR-HSA-5694335 (Reactome)
Factor V precursor ProteinP12259 (Uniprot-TrEMBL)
Factor VIII precursor ProteinP00451 (Uniprot-TrEMBL)
GDP MetaboliteCHEBI:17552 (ChEBI)
GDPMetaboliteCHEBI:17552 (ChEBI)
GOLGA2 ProteinQ08379 (Uniprot-TrEMBL)
GOLGA2:GORASP1ComplexR-HSA-5694327 (Reactome)
GORASP1 ProteinQ9BQQ3 (Uniprot-TrEMBL)
GOSR2 ProteinO14653 (Uniprot-TrEMBL)
GOSR2ProteinO14653 (Uniprot-TrEMBL)
GPI-CD59 ProteinP13987 (Uniprot-TrEMBL)
GPI-FOLR1 ProteinP15328 (Uniprot-TrEMBL)
GRIA1 ProteinP42261 (Uniprot-TrEMBL)
GTP MetaboliteCHEBI:15996 (ChEBI)
GTPMetaboliteCHEBI:15996 (ChEBI)
GalHyl-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
GlcGalHyl-COL7A1 ProteinQ02388 (Uniprot-TrEMBL)
Glycoproteins with Man8 N-glycans R-HSA-5694207 (Reactome)
Glycoproteins with Man8 N-glycans R-HSA-5694216 (Reactome)
Glycoproteins with Man8 N-glycans R-HSA-5694224 (Reactome)
H2OMetaboliteCHEBI:15377 (ChEBI)
LMAN1 ProteinP49257 (Uniprot-TrEMBL)
LMAN1L ProteinQ9HAT1 (Uniprot-TrEMBL)
LMAN2 ProteinQ12907 (Uniprot-TrEMBL)
LMAN2L ProteinQ9H0V9 (Uniprot-TrEMBL)
MCFD2 ProteinQ8NI22 (Uniprot-TrEMBL)
NAPA ProteinP54920 (Uniprot-TrEMBL)
NAPB ProteinQ9H115 (Uniprot-TrEMBL)
NAPG ProteinQ99747 (Uniprot-TrEMBL)
NSF ProteinP46459 (Uniprot-TrEMBL)
NSF hexamerComplexR-HSA-2193131 (Reactome)
PP6ComplexR-HSA-5694294 (Reactome)
PPP6C ProteinO00743 (Uniprot-TrEMBL)
PPP6R1 ProteinQ9UPN7 (Uniprot-TrEMBL)
PPP6R3 ProteinQ5H9R7 (Uniprot-TrEMBL)
PREBProteinQ9HCU5 (Uniprot-TrEMBL)
PalmC-YKT6 ProteinO15498 (Uniprot-TrEMBL)
PiMetaboliteCHEBI:18367 (ChEBI)
RAB1:GDPComplexR-HSA-5694296 (Reactome)
RAB1:GTP:TBC1D20ComplexR-HSA-6814832 (Reactome)
RAB1:GTPComplexR-HSA-6807799 (Reactome)
RAB1A ProteinP62820 (Uniprot-TrEMBL)
RAB1B ProteinQ9H0U4 (Uniprot-TrEMBL)
SAR1B ProteinQ9Y6B6 (Uniprot-TrEMBL)
SAR1B:GDPComplexR-HSA-203989 (Reactome)
SAR1B:GTPComplexR-HSA-204003 (Reactome)
SAR1BProteinQ9Y6B6 (Uniprot-TrEMBL)
SCFD1 ProteinQ8WVM8 (Uniprot-TrEMBL)
SCFD1ProteinQ8WVM8 (Uniprot-TrEMBL)
SEC13 ProteinP55735 (Uniprot-TrEMBL)
SEC16A ProteinO15027 (Uniprot-TrEMBL)
SEC16A:SEC16BComplexR-HSA-5694307 (Reactome)
SEC16B ProteinQ96JE7 (Uniprot-TrEMBL)
SEC22A ProteinQ96IW7 (Uniprot-TrEMBL)
SEC22B ProteinO75396 (Uniprot-TrEMBL)
SEC22C ProteinQ9BRL7 (Uniprot-TrEMBL)
SEC23:SEC24ComplexR-HSA-203975 (Reactome)
SEC23A ProteinQ15436 (Uniprot-TrEMBL)
SEC23IP ProteinQ9Y6Y8 (Uniprot-TrEMBL)
SEC24A ProteinO95486 (Uniprot-TrEMBL)
SEC24B ProteinO95487 (Uniprot-TrEMBL)
SEC24C ProteinP53992 (Uniprot-TrEMBL)
SEC24D ProteinO94855 (Uniprot-TrEMBL)
SEC24ComplexR-HSA-203991 (Reactome)
SEC31A ProteinO94979 (Uniprot-TrEMBL)
SEC31A:SEC13:SEC23IPComplexR-HSA-5694316 (Reactome)
SNAPsComplexR-HSA-5694313 (Reactome)
STX17 ProteinP56962 (Uniprot-TrEMBL)
STX5 ProteinQ13190 (Uniprot-TrEMBL)
STX5,17ComplexR-HSA-6807784 (Reactome)
TBC1D20 ProteinQ96BZ9 (Uniprot-TrEMBL)
TBC1D20ProteinQ96BZ9 (Uniprot-TrEMBL)
TFG ProteinQ92734 (Uniprot-TrEMBL)
TFG hexamerComplexR-HSA-5694310 (Reactome)
TGFA precursor ProteinP01135 (Uniprot-TrEMBL)
TMED10 ProteinP49755 (Uniprot-TrEMBL)
TMED2 ProteinQ15363 (Uniprot-TrEMBL)
TRAPPC1 ProteinQ9Y5R8 (Uniprot-TrEMBL)
TRAPPC10 ProteinP48553 (Uniprot-TrEMBL)
TRAPPC2 ProteinP0DI81 (Uniprot-TrEMBL)
TRAPPC2L ProteinQ9UL33 (Uniprot-TrEMBL)
TRAPPC3 ProteinO43617 (Uniprot-TrEMBL)
TRAPPC4 ProteinQ9Y296 (Uniprot-TrEMBL)
TRAPPC5 ProteinQ8IUR0 (Uniprot-TrEMBL)
TRAPPC6A ProteinO75865 (Uniprot-TrEMBL)
TRAPPC6B ProteinQ86SZ2 (Uniprot-TrEMBL)
TRAPPC9 ProteinQ96Q05 (Uniprot-TrEMBL)
TRAPPCIIComplexR-HSA-5694315 (Reactome)
USO1 ProteinO60763 (Uniprot-TrEMBL)
USO1 homodimerComplexR-HSA-5694302 (Reactome)
cis-SNARE:NSF hexamer:3xSNAPComplexR-HSA-5694312 (Reactome)
cis-SNAREComplexR-HSA-5694303 (Reactome)
nascent procollagen VII vesicle R-ALL-5694171 (Reactome)
p-S742,T747 SEC23AProteinQ15436 (Uniprot-TrEMBL)
p-SEC24A ProteinO95486 (Uniprot-TrEMBL)
p-SEC24B ProteinO95487 (Uniprot-TrEMBL)
p-SEC24C ProteinP53992 (Uniprot-TrEMBL)
p-SEC24D ProteinO94855 (Uniprot-TrEMBL)
p-SEC24ComplexR-HSA-5694187 (Reactome)

Annotated Interactions

View all...
SourceTargetTypeDatabase referenceComment
ADPArrowR-HSA-5694441 (Reactome)
ATPR-HSA-5694425 (Reactome)
ATPR-HSA-5694441 (Reactome)
BET1:GOSR2:STX5:SCFD1R-HSA-5694446 (Reactome)
BET1ArrowR-HSA-5694425 (Reactome)
COPII

cargo

receptors:SAR1B:GTP:SEC23:SEC24
ArrowR-HSA-5694522 (Reactome)
COPII

cargo

receptors:SAR1B:GTP:SEC23:SEC24
R-HSA-5694417 (Reactome)
COPII

cargo

receptors:SAR1B:GTP:SEC23:SEC24
R-HSA-5694527 (Reactome)
COPII

cargo

receptors:SAR1B:GTP:SEC23:SEC24
mim-catalysisR-HSA-5694527 (Reactome)
COPII cargo:receptors:SAR1B:GTP:SEC23:SEC24:SEC16A:SEC16B:TFG hexamer:SEC13:SEC31:SEC23IP:v-SNARESArrowR-HSA-204008 (Reactome)
COPII cargo:receptors:SAR1B:GTP:SEC23:SEC24:SEC16A:SEC16B:TFG hexamer:SEC13:SEC31:SEC23IP:v-SNARESR-HSA-203973 (Reactome)
COPII cargo:receptors:SAR1B:GTP:SEC23:SEC24:SEC16A:SEC16B:TFG hexamer:SEC13:SEC31:SEC23IP:v-SNARESmim-catalysisR-HSA-203973 (Reactome)
COPII cargo:receptors:SAR1B:GTP:SEC23:SEC24:SEC16A:SEC16B:TFG hexamerArrowR-HSA-5694417 (Reactome)
COPII cargo:receptors:SAR1B:GTP:SEC23:SEC24:SEC16A:SEC16B:TFG hexamerR-HSA-204008 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GDPArrowR-HSA-5694439 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GDPR-HSA-5694409 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GDPmim-catalysisR-HSA-5694409 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GTP:USO1 homodimer:GOLGA2:GORASP1ArrowR-HSA-5694418 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GTP:USO1 homodimer:GOLGA2:GORASP1R-HSA-5694441 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GTPArrowR-HSA-5694409 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREs:TRAPPCII:RAB1:GTPR-HSA-5694418 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREsArrowR-HSA-203973 (Reactome)
COPII cargo:receptors:SEC23:SEC24:SEC13:SEC31:SEC23IP:v-SNAREsR-HSA-5694439 (Reactome)
COPII cargo:receptors:v:SNAREs:GOLGA2:GORASP1:USO1 homodimerArrowR-HSA-5694441 (Reactome)
COPII cargo:receptors:v:SNAREs:GOLGA2:GORASP1:USO1 homodimerR-HSA-5694446 (Reactome)
COPII cargo:receptorsArrowR-HSA-5694446 (Reactome)
COPII cargo:receptorsArrowR-HSA-5694527 (Reactome)
COPII cargo:receptorsR-HSA-5694522 (Reactome)
CSNK1Dmim-catalysisR-HSA-5694441 (Reactome)
ER-to-ERGIC v-SNARESArrowR-HSA-5694425 (Reactome)
ER-to-ERGIC v-SNARESR-HSA-204008 (Reactome)
GDPArrowR-HSA-203977 (Reactome)
GDPArrowR-HSA-5694409 (Reactome)
GOLGA2:GORASP1ArrowR-HSA-5694446 (Reactome)
GOLGA2:GORASP1R-HSA-5694418 (Reactome)
GOSR2ArrowR-HSA-5694425 (Reactome)
GTPR-HSA-203977 (Reactome)
GTPR-HSA-5694409 (Reactome)
H2OR-HSA-5694421 (Reactome)
NSF hexamerArrowR-HSA-5694425 (Reactome)
NSF hexamerR-HSA-5694423 (Reactome)
PP6mim-catalysisR-HSA-5694421 (Reactome)
PREBmim-catalysisR-HSA-203977 (Reactome)
PiArrowR-HSA-203973 (Reactome)
PiArrowR-HSA-5694421 (Reactome)
PiArrowR-HSA-5694425 (Reactome)
PiArrowR-HSA-5694527 (Reactome)
R-HSA-203973 (Reactome) Once loaded the vesicles become fully sculpted, pinch off from the ER and bud into the cytosol. Budding depends on SAR1:GTPase activity and likely releases the SEC16 complex. SAR1:GTPase activity may result in partial uncoating of the emerging vesicle, allowing SEC23 to be exposed for subsequent TRAPPCII binding.
R-HSA-203977 (Reactome) SAR1-GDP is recruited to the ER membrane by the transmembrane GEF (Guanine nucleotide exchange factor) PREB (also known as SEC12), where it is converted to SAR1-GTP. PREB itself is recruited to ER exit sites through interaction with CTAGE5.
R-HSA-204008 (Reactome) The outer coat proteins SEC13 and SEC31A are thought to exist in a cytosolic heterohexamer with SEC23IP and are recruited to the ER through interaction with pre-bound SEC23-SEC24 complexes (Stephens et al, 2000; Hammond and Glick, 2000; Ong et al, 2010; reviewed in Szul and Sztul, 2011). v-SNAREs such as the SEC22 proteins are also incorporated into the emerging vesicle through interactions with components of the COPII coat (Xu et al, 2000; Mancias and Goldberg, 2008; Gordon et al, 2010; reviewed in Szul and Sztul, 2011; Lord et al, 2013).
R-HSA-5694409 (Reactome) The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it (Yamasaki et al, 2009; reviewed in Brunet and Sacher, 2014).
R-HSA-5694417 (Reactome) The multimeric SEC16 complex marks sites of ER exit (ERES) and helps to localize nascent COPII coat complexes through interaction with SEC23 and SAR1 (Battacharyya et al, 2007; Watson et al, 2006; Hughes et al, 2009; Yorimitsu and Sato, 2012; reviewed in Sprangers and Rabouille, 2015). SEC16 may help to prevent premature dissociation of the COPII coats after activation of SEC13 GTPase (Supek et al, 2002; Kung et al, 2012). SEC16 functions with hexameric TFG1, which is recruited to the ERES through direct interaction with SEC16 and is required for cargo traffic out of the ER and for organization of the ER-to-ERGIC boundary (Witte et al, 2001; Beetz et al, 2012; Johnson et al, 2015).
R-HSA-5694418 (Reactome) Protein protein interactions involving activated RAB1:GTP help dock the ER-derived vesicle on the cis-Golgi membrane (reviewed in Lord et al, 2013) . Independently, RAB:GTP interacts with both the USO1 homodimer (a long coiled coil tethering factor also known as p115) and the GOLGA2 component of the GOLGA2:GORASP1 complex on the cis-Golgi membrane (Allan et al, 2000; Moyer et al, 2001; Weide et al, 2001). USO1 also contacts GOLGA2 directly (Nakamura et al, 1997; Seeman et al, 2000). t-SNARES such as STX5, GOSR1and GOSR2 also participate in this complex on the cis-ERGIC membrane (Brandon et al, 2006; Shorter et al, 2002; reviewed in Brandizzi and Barlowe, 2013).
R-HSA-5694421 (Reactome) PP6 dephosphorylates released coat proteins including SEC24, SEC31 and SEC23, allowing them to recycle for further rounds of vesicle budding from the ER (Lord et al, 2011; Bhandari et al, 2013).
R-HSA-5694423 (Reactome) After membrane fusion, the cis-SNARE complex is dissociated in an ATP-dependent fashion by the AAA protein NSF in conjunction with SNAP proteins (Mayer et al, 1996; Sollner et al, 1993; reviewed in Jahn and Scheller, 2006; Sudhof and Rothman, 2009).
R-HSA-5694425 (Reactome) NSF-dependent hydrolysis of ATP is required to disassociate the cis-SNARE complex, releasing the SNAREs for further rounds of membrane fusion (Mayer et al, 1996; Muller et al, 1999; Muller et al, 2002; Otto et al, 1997; Whiteheart et al, 2004; Yu et al, 1999; Zhao et al, 2012; Shah et al, 2015; reviewed in Sudhof and Rothman, 2009).
R-HSA-5694439 (Reactome) TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic (reviewed in Barrowman et al, 2010; Brunet and Sacher, 2014). TRAPPC is a guanine-nucleotide exchange factor for RAB1 and is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPC component TRAPPC3 and the coat protein SEC23 (Cai et al, 2007; Lord et al, 2011).
R-HSA-5694441 (Reactome) Coat release at the ERGIC membrane may be stimulated by CSNK1D-dependent phosphorylation of SEC23 and SEC24 (Lord et al, 2011).
R-HSA-5694446 (Reactome) After displacement of the COPII coat, a tetrameric SNAREpin forms between the v-SNARE and the three t-SNARE proteins (Hay et al, 1997; Hay et al, 1998; Hui et al, 1997; Bentley et al, 2006; reviewed in Hong and Lev 2014). This interaction is promoted by the tethering factor USO1 and by the STX5-interacting protein SCFD1 (Wang et al, 2014; Dascher and Balch, 1996; Cao and Barlowe 2000; Flanagan and Barlowe, 2006; reviewed in Lord et al, 2013). The zippering of the SNAREpin provides the mechanical force that drives membrane fusion, releasing cargo into the acceptor compartment (reviewed in Sudhof and Rothman, 2009).
R-HSA-5694522 (Reactome) SAR1:GTP recruits the cytoplasmic SEC23:SEC24 complex. SEC24, and to a lesser extent SEC23, also mediate interaction with COPII cargo, concentrating it into the emerging vesicle. Transmembrane cargo may interact directly with the inner coat proteins, often in an isoform specific manner; alternately, some transmembrane proteins and all soluble cargo interact first with a cargo receptor of the p24, LMAN or ERV families.
R-HSA-5694527 (Reactome) Sar1p-GTP hydrolysis is increased 15-30-fold by Sec23p. Sar1p-GDP is released as a result of this hydrolysis and used in further vesicle sculpting cycles. Sar1p-GTP hydrolysis occurs at two critical points during the cycle, first (as represented here) as a proofreading step, insuring that the cargo is loaded. Later in the cycle Sar1p-GTP hydrolysis triggers the uncoating of the budded vesicle.
R-HSA-6814831 (Reactome) TBC1D20 was identified as a GTPase activating protein for RAB1 that stimulates basal GTP hydrolysis by more than 5 orders of magnitude (Haas et al, 2007; Fuchs et al, 2007).
R-HSA-6814833 (Reactome) TBC1D20 was identified as a RAB1-specific GTPase activating protein (GAP) that stimulates RAB1-mediated GTP hydrolysis and plays roles in ER-to-Golgi trafficking. TBC1D20 is the only GAP that has been identified to block delivery of secretory cargo from the ER to the cell surface (Haas et al, 2007).
RAB1:GDPArrowR-HSA-6814833 (Reactome)
RAB1:GDPR-HSA-5694439 (Reactome)
RAB1:GTP:TBC1D20ArrowR-HSA-6814831 (Reactome)
RAB1:GTP:TBC1D20R-HSA-6814833 (Reactome)
RAB1:GTP:TBC1D20mim-catalysisR-HSA-6814833 (Reactome)
RAB1:GTPArrowR-HSA-5694441 (Reactome)
RAB1:GTPR-HSA-6814831 (Reactome)
SAR1B:GDPArrowR-HSA-5694527 (Reactome)
SAR1B:GDPR-HSA-203977 (Reactome)
SAR1B:GTPArrowR-HSA-203977 (Reactome)
SAR1B:GTPR-HSA-5694522 (Reactome)
SAR1BArrowR-HSA-203973 (Reactome)
SCFD1ArrowR-HSA-5694425 (Reactome)
SEC16A:SEC16BArrowR-HSA-203973 (Reactome)
SEC16A:SEC16BR-HSA-5694417 (Reactome)
SEC23:SEC24ArrowR-HSA-5694527 (Reactome)
SEC23:SEC24R-HSA-5694522 (Reactome)
SEC24ArrowR-HSA-5694421 (Reactome)
SEC31A:SEC13:SEC23IPArrowR-HSA-5694441 (Reactome)
SEC31A:SEC13:SEC23IPR-HSA-204008 (Reactome)
SNAPsArrowR-HSA-5694425 (Reactome)
SNAPsR-HSA-5694423 (Reactome)
STX5,17ArrowR-HSA-5694425 (Reactome)
TBC1D20ArrowR-HSA-6814833 (Reactome)
TBC1D20R-HSA-6814831 (Reactome)
TFG hexamerArrowR-HSA-203973 (Reactome)
TFG hexamerR-HSA-5694417 (Reactome)
TRAPPCIIArrowR-HSA-5694441 (Reactome)
TRAPPCIIR-HSA-5694439 (Reactome)
USO1 homodimerArrowR-HSA-5694446 (Reactome)
USO1 homodimerR-HSA-5694418 (Reactome)
cis-SNARE:NSF hexamer:3xSNAPArrowR-HSA-5694423 (Reactome)
cis-SNARE:NSF hexamer:3xSNAPR-HSA-5694425 (Reactome)
cis-SNARE:NSF hexamer:3xSNAPmim-catalysisR-HSA-5694425 (Reactome)
cis-SNAREArrowR-HSA-5694446 (Reactome)
cis-SNARER-HSA-5694423 (Reactome)
p-S742,T747 SEC23AArrowR-HSA-5694441 (Reactome)
p-SEC24ArrowR-HSA-5694441 (Reactome)
p-SEC24R-HSA-5694421 (Reactome)
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